TIR3_YEAST
ID TIR3_YEAST Reviewed; 269 AA.
AC P40552; D6VVR8;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Cell wall protein TIR3;
DE AltName: Full=TIP1-related protein 3;
DE Flags: Precursor;
GN Name=TIR3; Synonyms=YIB1; OrderedLocusNames=YIL011W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=10383953; DOI=10.1128/jb.181.13.3886-3889.1999;
RA Hamada K., Terashima H., Arisawa M., Yabuki N., Kitada K.;
RT "Amino acid residues in the omega-minus region participate in cellular
RT localization of yeast glycosylphosphatidylinositol-attached proteins.";
RL J. Bacteriol. 181:3886-3889(1999).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=11292809; DOI=10.1128/jb.183.9.2881-2887.2001;
RA Abramova N.E., Sertil O., Mehta S., Lowry C.V.;
RT "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene
RT expression in Saccharomyces cerevisiae.";
RL J. Bacteriol. 183:2881-2887(2001).
CC -!- FUNCTION: Component of the cell wall. Required for anaerobic growth.
CC {ECO:0000269|PubMed:11292809}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000305|PubMed:10383953}. Membrane {ECO:0000305}; Lipid-anchor,
CC GPI-anchor {ECO:0000305}.
CC -!- INDUCTION: Induced during anaerobic growth.
CC {ECO:0000269|PubMed:11292809}.
CC -!- PTM: Extensively O-glycosylated. {ECO:0000250}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
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DR EMBL; Z38113; CAA86238.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08534.1; -; Genomic_DNA.
DR PIR; S48444; S48444.
DR RefSeq; NP_012254.1; NM_001179361.1.
DR AlphaFoldDB; P40552; -.
DR SMR; P40552; -.
DR BioGRID; 34979; 29.
DR DIP; DIP-1895N; -.
DR IntAct; P40552; 1.
DR MINT; P40552; -.
DR STRING; 4932.YIL011W; -.
DR PaxDb; P40552; -.
DR EnsemblFungi; YIL011W_mRNA; YIL011W; YIL011W.
DR GeneID; 854804; -.
DR KEGG; sce:YIL011W; -.
DR SGD; S000001273; TIR3.
DR VEuPathDB; FungiDB:YIL011W; -.
DR eggNOG; ENOG502RZ9R; Eukaryota.
DR GeneTree; ENSGT00940000176276; -.
DR HOGENOM; CLU_071083_1_1_1; -.
DR InParanoid; P40552; -.
DR OMA; YISYAEN; -.
DR BioCyc; YEAST:G3O-31288-MON; -.
DR PRO; PR:P40552; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40552; protein.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0005199; F:structural constituent of cell wall; IBA:GO_Central.
DR GO; GO:0031505; P:fungal-type cell wall organization; IBA:GO_Central.
DR InterPro; IPR000992; SRP1_TIP1.
DR Pfam; PF00660; SRP1_TIP1; 1.
DR PROSITE; PS00724; SRP1_TIP1; 1.
PE 2: Evidence at transcript level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..245
FT /note="Cell wall protein TIR3"
FT /id="PRO_0000033236"
FT PROPEP 246..269
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000372446"
FT REGION 128..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 245
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 234
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 269 AA; 26308 MW; 48AF914D8C149124 CRC64;
MSFTKIAALL AVAAASTQLV SAEVGQYEIV EFDAILADVK ANLEQYMSLA MNNPDFTLPS
GVLDVYQHMT TATDDSYTSY FTEMDFAQIT TAMVQVPWYS SRLEPEIIAA LQSAGISITS
LGQTVSESGS ESATASSDAS SASESSSAAS SSASESSSAA SSSASESSSA ASSSASESSS
AASSSASEAA KSSSSAKSSG SSAASSAASS ASSKASSAAS SSAKASSSAE KSTNSSSSAT
SKNAGAAMDM GFFSAGVGAA IAGAAAMLL
