BSL1_ORYSJ
ID BSL1_ORYSJ Reviewed; 883 AA.
AC Q60EX6; Q0DKT4;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Serine/threonine-protein phosphatase BSL1 homolog;
DE EC=3.1.3.16;
DE AltName: Full=BSU1-like protein 1 homolog;
GN Name=BSL1; OrderedLocusNames=Os05g0144400, LOC_Os05g05240;
GN ORFNames=OJ1607_F09.10;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP INTERACTION WITH BSK3.
RX PubMed=26697897; DOI=10.1104/pp.15.01668;
RA Zhang B., Wang X., Zhao Z., Wang R., Huang X., Zhu Y., Yuan L., Wang Y.,
RA Xu X., Burlingame A.L., Gao Y., Sun Y., Tang W.;
RT "OsBRI1 activates BR signaling by preventing binding between the TPR and
RT kinase domains of OsBSK3 via phosphorylation.";
RL Plant Physiol. 170:1149-1161(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CC -!- SUBUNIT: Interacts with the phosphorylated form of BSK3.
CC {ECO:0000305|PubMed:26697897}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PPP phosphatase family. BSU subfamily.
CC {ECO:0000305}.
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DR EMBL; AC104283; AAU90203.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF16539.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS92232.1; -; Genomic_DNA.
DR EMBL; AK120211; BAG99920.1; -; mRNA.
DR RefSeq; XP_015640150.1; XM_015784664.1.
DR AlphaFoldDB; Q60EX6; -.
DR SMR; Q60EX6; -.
DR STRING; 4530.OS05T0144400-01; -.
DR PaxDb; Q60EX6; -.
DR PRIDE; Q60EX6; -.
DR EnsemblPlants; Os05t0144400-01; Os05t0144400-01; Os05g0144400.
DR GeneID; 4337785; -.
DR Gramene; Os05t0144400-01; Os05t0144400-01; Os05g0144400.
DR KEGG; osa:4337785; -.
DR eggNOG; KOG0374; Eukaryota.
DR eggNOG; KOG0379; Eukaryota.
DR HOGENOM; CLU_004962_7_1_1; -.
DR InParanoid; Q60EX6; -.
DR OMA; YGFQDEC; -.
DR OrthoDB; 124339at2759; -.
DR PlantReactome; R-OSA-5632095; Brassinosteroid signaling.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q60EX6; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR GO; GO:0009742; P:brassinosteroid mediated signaling pathway; IEA:InterPro.
DR CDD; cd07419; MPP_Bsu1_C; 1.
DR Gene3D; 2.120.10.80; -; 2.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR041758; MPP_BSL_C.
DR InterPro; IPR006186; Ser/Thr-sp_prot-phosphatase.
DR InterPro; IPR012391; Ser/Thr_prot_Pase_BSU1.
DR Pfam; PF00149; Metallophos; 1.
DR PIRSF; PIRSF036363; PPP_BSU1; 1.
DR PRINTS; PR00114; STPHPHTASE.
DR SMART; SM00156; PP2Ac; 1.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
DR PROSITE; PS00125; SER_THR_PHOSPHATASE; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Kelch repeat; Manganese; Metal-binding; Nucleus;
KW Protein phosphatase; Reference proteome; Repeat.
FT CHAIN 1..883
FT /note="Serine/threonine-protein phosphatase BSL1 homolog"
FT /id="PRO_0000247484"
FT REPEAT 64..113
FT /note="Kelch 1"
FT REPEAT 221..271
FT /note="Kelch 2"
FT REPEAT 273..323
FT /note="Kelch 3"
FT REPEAT 341..387
FT /note="Kelch 4"
FT REGION 381..402
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 430..466
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 499..525
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 861..883
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 430..451
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 653
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 586
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 588
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 620
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 620
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 652
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 705
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 784
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 883 AA; 96282 MW; F75CF327E3815863 CRC64;
MGTAGKGAWV VPAPAYREVE GWEGAGDDSP GFRCGHSLTV VAPTKGHGPR LILFGGATAI
EAGASSGMPG IRLAGVTNSV HSYDVDTRRW TRLHPAGEPP SPRAAHAAAA VGTMVVFQGG
IGPAGHSTDD LYVLDLTNDK FKWHRVVVQG AGPGPRYGHC MDLVAQRYLV TVSGNDGKRV
LSDAWALDTA QKPYRWQKLN PDGDRPSARM YATASARTDG MLLLCGGRDA SGMPLSDAYG
LLMHTSGQWE WTLAPGVSPS PRYQHAAVFV GARLHVTGGV LRGGRAIEGE GAIAVLDTAA
GVWLDRNGIV TSRTLKSSHD HDASSDLLRR CRHAAASVGT QIYIYGGLRG DILLDDFLVA
DNAPIQSEFT SSMYDRVPRA ENQNRNHNFN SDSPTTNNST DKKSIDMLTQ ASAAEAEAVS
AVWRAAQEAS HASSEDSLSE GIGSESPLSE TSPMPEDLDD GGSLEPDVKL HSRAVVVSKE
AVGDLGCLVR QLSLDQFENE SRRMHPSSND QSYPAKKALN RQRSPQGLHK KVISFLLKPR
NWRAPAERAF FLDSYEVGEL CYAAEQIFMQ EPTVLQLKAP IKVFGDLHGQ FGDLMRLFDE
YGYPSTAGDI TYIDYLFLGD YVDRGQHSLE TITLLLALKI EYPENVHLIR GNHEAADINA
LFGFRLECIE RMGESDGIWA WTRFNQLFNY LPLAAMIEKK IICMHGGIGR SINTIEQIEK
LERPITMDVG SIILMDLLWS DPTENDSVEG LRPNARGPGL VTFGPDRVTE FCKRNRLQLI
IRAHECVMDG FERFAHGQLI TLFSATNYCG TANNAGAILV VGRGLVIVPK LIHPLPPPVN
SPESSPERAM DATWMQELNI QRPPTPTRGR PQSASDRNSL AYI
