ID   TKDP1_BOVIN             Reviewed;         351 AA.
AC   Q28201; Q9N0X8;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2003, sequence version 2.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Trophoblast Kunitz domain protein 1;
DE            Short=TKDP-1;
DE   Flags: Precursor;
GN   Name=TKDP1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Hereford X Simmental;
RA   Green J.A., Maclean J.A. II, Gan X., Xie S., Roberts R.M.;
RT   "Cloning and characterization of the expression of trophoblast Kunitz
RT   domain proteins (TKDP).";
RL   Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 188-351.
RX   PubMed=7510284; DOI=10.1016/s0021-9258(17)37276-9;
RA   Kramer K.K., Duffy J.Y., Klemann S.W., Bixby J.A., Low B.G., Pope W.F.,
RA   Roberts R.M.;
RT   "Selective cloning of cDNA for secretory proteins of early embryos.
RT   Identification of a transiently expressed Kunitz domain protein from
RT   preimplantation sheep trophoblast.";
RL   J. Biol. Chem. 269:7255-7261(1994).
CC   -!- FUNCTION: May play a role in mediating maternal-conceptus interactions
CC       in the immediate preimplantation period. Does not seem to have
CC       proteinase inhibitory activity (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DEVELOPMENTAL STAGE: Maximal expression at days 17-19 of pregnancy.
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DR   EMBL; AF241776; AAF61247.1; -; mRNA.
DR   EMBL; L11344; AAA30795.1; -; mRNA.
DR   RefSeq; NP_991345.1; NM_205776.1.
DR   AlphaFoldDB; Q28201; -.
DR   SMR; Q28201; -.
DR   STRING; 9913.ENSBTAP00000052527; -.
DR   GeneID; 404076; -.
DR   KEGG; bta:404076; -.
DR   CTD; 404076; -.
DR   eggNOG; KOG4597; Eukaryota.
DR   OrthoDB; 483422at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..351
FT                   /note="Trophoblast Kunitz domain protein 1"
FT                   /id="PRO_0000016879"
FT   REPEAT          32..116
FT                   /note="1"
FT   REPEAT          117..201
FT                   /note="2"
FT   REPEAT          202..286
FT                   /note="3"
FT   DOMAIN          294..344
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   REGION          32..286
FT                   /note="3 X 84 AA approximate tandem repeats"
FT   SITE            304..305
FT                   /note="Reactive bond homolog"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        265
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        294..344
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        303..327
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        319..340
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   CONFLICT        274
FT                   /note="L -> P (in Ref. 2; AAA30795)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        316
FT                   /note="N -> T (in Ref. 2; AAA30795)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   351 AA;  38255 MW;  13DBAF9AA25FEB35 CRC64;
     MRQLCLSSAL LFLLVILVDS TPLNIHHIQD EGVETSHRRG PEKRSVIDVV TSIIDGVATG
     TKIVKNGAGL LTGLAEIITK AIKGQVMISR IQFDNHTQEE LPTLNIEYST LSEENKGVET
     SHRKGPEKRS VIDVVTSIID GVATGTKIVT KGASILTGLA EIINKAIRGQ VMISRIQFNN
     HTLEEFPKLN IEYSTLSEDN TGVETSRRRG PEKRSVIDVV TSIIDGVATG TKIVKKGTSI
     LTGLAEIINK AIKGQVMISG IQFNNHTLEE YQTLKIEYSA LNEENKAASK PALCLEPKVT
     GGCNAVMTRY FYNAQNGLCE QFVYDGCEGN GNNFEKLEDC MKTCSQEAGS L