TKI1_ARATH
ID TKI1_ARATH Reviewed; 743 AA.
AC Q8LJT8; Q3EBL6; Q8VZJ8; Q9SJL1;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=TSL-kinase interacting protein 1;
DE AltName: Full=Myb-related protein TKI1;
GN Name=TKI1; OrderedLocusNames=At2g36960; ORFNames=T1J8.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH TOUSLED,
RP PHOSPHORYLATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=15047893; DOI=10.1104/pp.103.038117;
RA Ehsan H., Reichheld J.-P., Durfee T., Roe J.L.;
RT "TOUSLED kinase activity oscillates during the cell cycle and interacts
RT with chromatin regulators.";
RL Plant Physiol. 134:1488-1499(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP INTERACTION WITH SNL1.
RX PubMed=19962994; DOI=10.1016/j.jmb.2009.11.065;
RA Bowen A.J., Gonzalez D., Mullins J.G., Bhatt A.M., Martinez A.,
RA Conlan R.S.;
RT "PAH-domain-specific interactions of the Arabidopsis transcription
RT coregulator SIN3-LIKE1 (SNL1) with telomere-binding protein 1 and ALWAYS
RT EARLY2 Myb-DNA binding factors.";
RL J. Mol. Biol. 395:937-949(2010).
CC -!- SUBUNIT: Interacts only with active kinase forms of TOUSLED. Interacts
CC with SNL1. {ECO:0000269|PubMed:15047893, ECO:0000269|PubMed:19962994}.
CC -!- INTERACTION:
CC Q8LJT8; Q39238: TOUSLED; NbExp=4; IntAct=EBI-2366507, EBI-2325484;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LJT8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LJT8-2; Sequence=VSP_022229;
CC -!- TISSUE SPECIFICITY: Expressed in flowers, roots and leaves.
CC {ECO:0000269|PubMed:15047893}.
CC -!- DOMAIN: The Myb domain is not required for TOUSLED binding.
CC -!- PTM: Phosphorylated in vitro by TOUSLED. {ECO:0000269|PubMed:15047893}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD31581.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF530160; AAM94622.1; -; mRNA.
DR EMBL; AC006922; AAD31581.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09326.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09327.2; -; Genomic_DNA.
DR EMBL; AY064068; AAL36424.1; -; mRNA.
DR EMBL; AY096376; AAM20017.1; -; mRNA.
DR PIR; G84786; G84786.
DR RefSeq; NP_001318368.1; NM_001336615.1. [Q8LJT8-1]
DR RefSeq; NP_850273.1; NM_179942.4. [Q8LJT8-1]
DR AlphaFoldDB; Q8LJT8; -.
DR BioGRID; 3614; 6.
DR IntAct; Q8LJT8; 7.
DR STRING; 3702.AT2G36960.3; -.
DR SwissPalm; Q8LJT8; -.
DR PaxDb; Q8LJT8; -.
DR PRIDE; Q8LJT8; -.
DR ProteomicsDB; 246409; -. [Q8LJT8-1]
DR EnsemblPlants; AT2G36960.1; AT2G36960.1; AT2G36960. [Q8LJT8-1]
DR EnsemblPlants; AT2G36960.2; AT2G36960.2; AT2G36960. [Q8LJT8-1]
DR GeneID; 818270; -.
DR Gramene; AT2G36960.1; AT2G36960.1; AT2G36960. [Q8LJT8-1]
DR Gramene; AT2G36960.2; AT2G36960.2; AT2G36960. [Q8LJT8-1]
DR KEGG; ath:AT2G36960; -.
DR Araport; AT2G36960; -.
DR eggNOG; KOG4468; Eukaryota.
DR InParanoid; Q8LJT8; -.
DR OMA; ADLYWAD; -.
DR PhylomeDB; Q8LJT8; -.
DR PRO; PR:Q8LJT8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8LJT8; baseline and differential.
DR Genevisible; Q8LJT8; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0007389; P:pattern specification process; IBA:GO_Central.
DR CDD; cd00167; SANT; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR SMART; SM00717; SANT; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51293; SANT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..743
FT /note="TSL-kinase interacting protein 1"
FT /id="PRO_0000270796"
FT DOMAIN 53..104
FT /note="SANT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00624"
FT REGION 486..523
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 626..679
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 500..523
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 649..665
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 286..287
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15047893"
FT /id="VSP_022229"
FT CONFLICT 2
FT /note="L -> V (in Ref. 1; AAM94622)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 743 AA; 82258 MW; B952325D7102ECCC CRC64;
MLKQFTHYCE MQAELIPEGP NGEGRLSNQN SNPNLLSSAS ISITQFPAKK PTRQWAAWTH
QEEESFFTAL RQVGKNFEKI TSRVQSKNKD QVRHYYYRLV RRMNKLLGPD LSLDAKNPKD
TNAAMLRWWS LLEKYSCKAS KLHLKPRRFK LFIEALEHQL LKDRRKSIRK RTCQGENLSS
ASLGNISSHS RERGLDNRPF KLILSDGQNV KKLGPGRAST KHGESLSVNL GDEKEDTAFG
RGGRQRRKQG YRKWEKAAID GVSLVADAAE HLERTSIDKD MDDQTDLGPT RYLTGKSPLS
LCSAGDVPLS DANMQFSAKL KLQLFPIDEC TRRSLEMDKH NPHLELTLSN RKKISSVLEH
LNRKWGSSSC ATGELLLFPY NARKETVTCH QRWTHDSFLS AAEVHSMVGS PSVFRLRYGW
FVHDASGSII SQVPTSDPCP SLEDDMNVDR LNEVNMLLTE SGPLSVHSTA EQTTSVEPSQ
GLVCASGVHD RPARSRDDYE PASTSITPLE HLSGGNAQSP GEWADSLTNI SIGDLLSEVP
DDIDSDGVDP PATEGSHYLL RDVPFTSDSF DAAIAAHILR HQNKPSAQLP LTSGSSSLWD
DEETRDAFSF QKNRFANSTE LASVASPKGV GRVNGEPSQL VEASSGDEGS YNPHDDGDPM
EEGPADPHTM DSPGKTPCGL ADVYWPDSLG PLDLDIRSSK YTDDLILSES LGGLSRLIAT
SLDAFQNCSL FGFDNKKDKS NMV
