TKN1_BOVIN
ID TKN1_BOVIN Reviewed; 130 AA.
AC P01289; A5PK57; B0JYM8; P01291; P04091; P20773;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Protachykinin-1;
DE AltName: Full=PPT;
DE Contains:
DE RecName: Full=Substance P;
DE Contains:
DE RecName: Full=Neurokinin A;
DE Short=NKA;
DE AltName: Full=Neuromedin L;
DE AltName: Full=Substance K;
DE Contains:
DE RecName: Full=Neuropeptide K;
DE Short=NPK;
DE Contains:
DE RecName: Full=Neuropeptide gamma;
DE Contains:
DE RecName: Full=C-terminal-flanking peptide;
DE Flags: Precursor;
GN Name=TAC1; Synonyms=NKA, NKNA, TAC2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), AND AMIDATION AT
RP MET-68 AND MET-107.
RX PubMed=6195531; DOI=10.1038/306032a0;
RA Nawa H., Hirose T., Takashima H., Inayama S., Nakanishi S.;
RT "Nucleotide sequences of cloned cDNAs for two types of bovine brain
RT substance P precursor.";
RL Nature 306:32-36(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND ALTERNATIVE SPLICING.
RX PubMed=6083453; DOI=10.1038/312729a0;
RA Nawa H., Kotani H., Nakanishi S.;
RT "Tissue-specific generation of two preprotachykinin mRNAs from one gene by
RT alternative RNA splicing.";
RL Nature 312:729-734(1984).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS BETA AND GAMMA).
RC STRAIN=Crossbred X Angus; TISSUE=Ileum, and Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 36-122 (ISOFORMS BETA AND GAMMA).
RC TISSUE=Hypothalamus;
RX PubMed=1708336; DOI=10.1210/endo-128-5-2441;
RA Chiwakata C., Brackmann B., Hunt N., Davidoff M., Schulze W., Ivell R.;
RT "Tachykinin (substance-P) gene expression in Leydig cells of the human and
RT mouse testis.";
RL Endocrinology 128:2441-2448(1991).
RN [5]
RP PROTEIN SEQUENCE OF 111-126 (ISOFORM BETA).
RX PubMed=2809597; DOI=10.1111/j.1471-4159.1989.tb09255.x;
RA McGregor G.P., Kage R., Thim L., Conlon J.M.;
RT "Quantitation and characterization of peptides from the C-terminal flanking
RT region of rat and bovine preprotachykinins.";
RL J. Neurochem. 53:1871-1877(1989).
CC -!- FUNCTION: Tachykinins are active peptides which excite neurons, evoke
CC behavioral responses, are potent vasodilators and secretagogues, and
CC contract (directly or indirectly) many smooth muscles.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=Beta;
CC IsoId=P01289-1; Sequence=Displayed;
CC Name=Alpha;
CC IsoId=P01289-2; Sequence=VSP_006373, VSP_006374;
CC Name=Gamma;
CC IsoId=P01289-3; Sequence=VSP_006372;
CC Name=Delta;
CC IsoId=P01289-4; Sequence=VSP_006372, VSP_006373, VSP_006374;
CC -!- PTM: [Substance P]: The substance P form is cleaved at Pro-59 by the
CC prolyl endopeptidase FAP (seprase) activity (in vitro). Substance P is
CC also cleaved and degraded by Angiotensin-converting enzyme (ACE) and
CC neprilysin (MME). {ECO:0000250|UniProtKB:P20366}.
CC -!- SIMILARITY: Belongs to the tachykinin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA24943.1; Type=Miscellaneous discrepancy; Evidence={ECO:0000305};
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DR EMBL; X00075; CAA24939.1; -; mRNA.
DR EMBL; X00075; CAA24940.1; -; mRNA.
DR EMBL; X00075; CAA24941.1; -; mRNA.
DR EMBL; X00076; CAA24942.1; -; mRNA.
DR EMBL; X00076; CAA24943.1; ALT_SEQ; mRNA.
DR EMBL; X02351; CAA26206.1; -; Genomic_DNA.
DR EMBL; X01396; CAA26206.1; JOINED; Genomic_DNA.
DR EMBL; X01397; CAA26206.1; JOINED; Genomic_DNA.
DR EMBL; X01398; CAA26206.1; JOINED; Genomic_DNA.
DR EMBL; X01399; CAA26206.1; JOINED; Genomic_DNA.
DR EMBL; X01400; CAA26206.1; JOINED; Genomic_DNA.
DR EMBL; BC142365; AAI42366.1; -; mRNA.
DR EMBL; BC151422; AAI51423.1; -; mRNA.
DR EMBL; M68911; AAA30724.1; -; mRNA.
DR EMBL; M68912; AAA30725.1; -; mRNA.
DR PIR; A05093; SPBOB.
DR PIR; JC5455; JC5455.
DR RefSeq; NP_776618.1; NM_174193.2. [P01289-1]
DR RefSeq; XP_005205182.1; XM_005205125.3. [P01289-3]
DR RefSeq; XP_005205183.1; XM_005205126.3. [P01289-2]
DR RefSeq; XP_005205184.1; XM_005205127.3. [P01289-4]
DR RefSeq; XP_010802283.1; XM_010803981.2. [P01289-1]
DR AlphaFoldDB; P01289; -.
DR STRING; 9913.ENSBTAP00000020419; -.
DR PaxDb; P01289; -.
DR Ensembl; ENSBTAT00000020419; ENSBTAP00000020419; ENSBTAG00000015356. [P01289-1]
DR Ensembl; ENSBTAT00000057037; ENSBTAP00000053014; ENSBTAG00000015356. [P01289-3]
DR GeneID; 281512; -.
DR KEGG; bta:281512; -.
DR CTD; 6863; -.
DR VEuPathDB; HostDB:ENSBTAG00000015356; -.
DR eggNOG; ENOG502S1KJ; Eukaryota.
DR GeneTree; ENSGT00390000002457; -.
DR HOGENOM; CLU_149426_0_0_1; -.
DR InParanoid; P01289; -.
DR OMA; NQIQDDW; -.
DR OrthoDB; 1504814at2759; -.
DR TreeFam; TF333405; -.
DR Proteomes; UP000009136; Chromosome 4.
DR Bgee; ENSBTAG00000015356; Expressed in midbrain and 47 other tissues.
DR ExpressionAtlas; P01289; baseline.
DR GO; GO:0030424; C:axon; ISS:AgBase.
DR GO; GO:0005576; C:extracellular region; ISS:AgBase.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0043025; C:neuronal cell body; ISS:AgBase.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0031835; F:substance P receptor binding; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IEA:UniProtKB-KW.
DR GO; GO:0006954; P:inflammatory response; ISS:AgBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISS:AgBase.
DR GO; GO:0048265; P:response to pain; ISS:AgBase.
DR GO; GO:0019233; P:sensory perception of pain; ISS:AgBase.
DR GO; GO:0007217; P:tachykinin receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR013055; Tachy_Neuro_lke_CS.
DR InterPro; IPR008215; Tachykinin_dom.
DR InterPro; IPR008216; Tachykinin_fam.
DR Pfam; PF02202; Tachykinin; 1.
DR PRINTS; PR01829; PROTACHYKNIN.
DR SMART; SM00203; TK; 2.
DR PROSITE; PS00267; TACHYKININ; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Amidation; Cleavage on pair of basic residues;
KW Direct protein sequencing; Neuropeptide; Neurotransmitter;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..56
FT /evidence="ECO:0000255"
FT /id="PRO_0000033522"
FT PEPTIDE 58..68
FT /note="Substance P"
FT /id="PRO_0000033523"
FT PEPTIDE 72..107
FT /note="Neuropeptide K"
FT /id="PRO_0000033524"
FT PEPTIDE 72..73
FT /note="Neuropeptide gamma, 1st part"
FT /id="PRO_0000033525"
FT PEPTIDE 89..107
FT /note="Neuropeptide gamma, 2nd part"
FT /id="PRO_0000033526"
FT PEPTIDE 98..107
FT /note="Neurokinin A"
FT /id="PRO_0000033527"
FT PEPTIDE 111..126
FT /note="C-terminal-flanking peptide"
FT /id="PRO_0000033528"
FT SITE 59..60
FT /note="Cleavage; by FAP"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT SITE 63..64
FT /note="Cleavage; by MME"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT SITE 64..65
FT /note="Cleavage; by MME"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT SITE 65..66
FT /note="Cleavage; by ACE"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT SITE 66..67
FT /note="Cleavage; by ACE and MME"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT MOD_RES 68
FT /note="Methionine amide"
FT /evidence="ECO:0000269|PubMed:6195531"
FT MOD_RES 107
FT /note="Methionine amide"
FT /evidence="ECO:0000269|PubMed:6195531"
FT VAR_SEQ 74..88
FT /note="Missing (in isoform Gamma and isoform Delta)"
FT /evidence="ECO:0000303|PubMed:1708336, ECO:0000303|Ref.3"
FT /id="VSP_006372"
FT VAR_SEQ 97..114
FT /note="Missing (in isoform Alpha and isoform Delta)"
FT /evidence="ECO:0000303|PubMed:6195531"
FT /id="VSP_006373"
FT VAR_SEQ 115
FT /note="V -> M (in isoform Alpha and isoform Delta)"
FT /evidence="ECO:0000303|PubMed:6195531"
FT /id="VSP_006374"
FT CONFLICT 121
FT /note="V -> A (in Ref. 4; AAA30724/AAA30725)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 130 AA; 15076 MW; CE2A28572305DEB7 CRC64;
MKILVAVAVI FFISTQLSAE EIGANDDFNY WSDWSDSDQI KEEMPEPFEH LLQRIARRPK
PQQFFGLMGK RDADSSIEKQ VALLKALYGH GQLSHKRHKT DSFVGLMGKR ALNSVAYERS
VMQDYERRRK
