TKN1_MOUSE
ID TKN1_MOUSE Reviewed; 130 AA.
AC P41539; Q00073;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Protachykinin-1;
DE AltName: Full=PPT;
DE Contains:
DE RecName: Full=Substance P;
DE Contains:
DE RecName: Full=Neurokinin A;
DE Short=NKA;
DE AltName: Full=Neuromedin L;
DE AltName: Full=Substance K;
DE Contains:
DE RecName: Full=Neuropeptide K;
DE Short=NPK;
DE Contains:
DE RecName: Full=Neuropeptide gamma;
DE Contains:
DE RecName: Full=C-terminal-flanking peptide;
DE Flags: Precursor;
GN Name=Tac1; Synonyms=Nka, Nkna, Tac2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
RC STRAIN=ICR; TISSUE=Brain;
RA Kako K., Munekata E., Hosaka M., Murakami K., Nakayama K.;
RT "Cloning and sequence analysis of mouse cDNAs encoding preprotachykinin A
RT and B.";
RL Biomed. Res. 14:253-259(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 36-122 (ISOFORMS BETA AND GAMMA).
RC TISSUE=Brain;
RX PubMed=1708336; DOI=10.1210/endo-128-5-2441;
RA Chiwakata C., Brackmann B., Hunt N., Davidoff M., Schulze W., Ivell R.;
RT "Tachykinin (substance-P) gene expression in Leydig cells of the human and
RT mouse testis.";
RL Endocrinology 128:2441-2448(1991).
CC -!- FUNCTION: Tachykinins are active peptides which excite neurons, evoke
CC behavioral responses, are potent vasodilators and secretagogues, and
CC contract (directly or indirectly) many smooth muscles.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=Beta;
CC IsoId=P41539-1; Sequence=Displayed;
CC Name=Alpha;
CC IsoId=P41539-3; Sequence=Not described;
CC Name=Gamma;
CC IsoId=P41539-2; Sequence=VSP_006379;
CC Name=Delta;
CC IsoId=P41539-4; Sequence=Not described;
CC -!- PTM: [Substance P]: The substance P form is cleaved at Pro-59 by the
CC prolyl endopeptidase FAP (seprase) activity (in vitro). Substance P is
CC also cleaved and degraded by Angiotensin-converting enzyme (ACE) and
CC neprilysin (MME). {ECO:0000250|UniProtKB:P20366}.
CC -!- SIMILARITY: Belongs to the tachykinin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D17584; BAA04508.1; -; mRNA.
DR EMBL; M68908; AAA39969.1; -; mRNA.
DR EMBL; M68909; AAA39970.1; -; mRNA.
DR CCDS; CCDS19906.1; -. [P41539-1]
DR CCDS; CCDS80486.1; -. [P41539-2]
DR PIR; I52526; I52526.
DR RefSeq; NP_033337.1; NM_009311.2. [P41539-1]
DR RefSeq; XP_006505090.1; XM_006505027.1. [P41539-1]
DR AlphaFoldDB; P41539; -.
DR STRING; 10090.ENSMUSP00000139347; -.
DR BindingDB; P41539; -.
DR iPTMnet; P41539; -.
DR PhosphoSitePlus; P41539; -.
DR PaxDb; P41539; -.
DR PRIDE; P41539; -.
DR ProteomicsDB; 259105; -. [P41539-1]
DR ProteomicsDB; 259106; -. [P41539-2]
DR ABCD; P41539; 1 sequenced antibody.
DR Antibodypedia; 2784; 626 antibodies from 39 providers.
DR DNASU; 21333; -.
DR Ensembl; ENSMUST00000184986; ENSMUSP00000138818; ENSMUSG00000061762. [P41539-2]
DR Ensembl; ENSMUST00000185187; ENSMUSP00000139347; ENSMUSG00000061762. [P41539-1]
DR GeneID; 21333; -.
DR KEGG; mmu:21333; -.
DR UCSC; uc009axc.1; mouse. [P41539-1]
DR CTD; 6863; -.
DR MGI; MGI:98474; Tac1.
DR VEuPathDB; HostDB:ENSMUSG00000061762; -.
DR eggNOG; ENOG502S1KJ; Eukaryota.
DR GeneTree; ENSGT00390000002457; -.
DR HOGENOM; CLU_149426_0_0_1; -.
DR InParanoid; P41539; -.
DR OMA; NQIQDDW; -.
DR PhylomeDB; P41539; -.
DR TreeFam; TF333405; -.
DR Reactome; R-MMU-380095; Tachykinin receptors bind tachykinins.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR BioGRID-ORCS; 21333; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Tac2; mouse.
DR PRO; PR:P41539; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; P41539; protein.
DR Bgee; ENSMUSG00000061762; Expressed in olfactory tubercle and 164 other tissues.
DR ExpressionAtlas; P41539; baseline and differential.
DR Genevisible; P41539; MM.
DR GO; GO:0030424; C:axon; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; IDA:MGI.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0043025; C:neuronal cell body; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0031836; F:neuromedin K receptor binding; ISO:MGI.
DR GO; GO:0048018; F:receptor ligand activity; ISO:MGI.
DR GO; GO:0031837; F:substance K receptor binding; ISO:MGI.
DR GO; GO:0031835; F:substance P receptor binding; ISO:MGI.
DR GO; GO:0019731; P:antibacterial humoral response; ISO:MGI.
DR GO; GO:0019732; P:antifungal humoral response; ISO:MGI.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; ISO:MGI.
DR GO; GO:0008306; P:associative learning; ISO:MGI.
DR GO; GO:1990090; P:cellular response to nerve growth factor stimulus; IDA:MGI.
DR GO; GO:0007268; P:chemical synaptic transmission; IEA:UniProtKB-KW.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; ISO:MGI.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; ISO:MGI.
DR GO; GO:0050965; P:detection of temperature stimulus involved in sensory perception of pain; ISO:MGI.
DR GO; GO:0006954; P:inflammatory response; IDA:MGI.
DR GO; GO:0045087; P:innate immune response; ISO:MGI.
DR GO; GO:0031640; P:killing of cells of another organism; ISO:MGI.
DR GO; GO:0007616; P:long-term memory; ISO:MGI.
DR GO; GO:0010459; P:negative regulation of heart rate; ISO:MGI.
DR GO; GO:1901215; P:negative regulation of neuron death; ISO:MGI.
DR GO; GO:0003085; P:negative regulation of systemic arterial blood pressure; ISO:MGI.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0045760; P:positive regulation of action potential; ISO:MGI.
DR GO; GO:0002675; P:positive regulation of acute inflammatory response; ISO:MGI.
DR GO; GO:2000854; P:positive regulation of corticosterone secretion; ISO:MGI.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI.
DR GO; GO:0010634; P:positive regulation of epithelial cell migration; ISO:MGI.
DR GO; GO:1902093; P:positive regulation of flagellated sperm motility; ISO:MGI.
DR GO; GO:0050671; P:positive regulation of lymphocyte proliferation; ISO:MGI.
DR GO; GO:1901216; P:positive regulation of neuron death; ISO:MGI.
DR GO; GO:0045778; P:positive regulation of ossification; ISO:MGI.
DR GO; GO:0035815; P:positive regulation of renal sodium excretion; ISO:MGI.
DR GO; GO:0046878; P:positive regulation of saliva secretion; ISO:MGI.
DR GO; GO:1904058; P:positive regulation of sensory perception of pain; ISO:MGI.
DR GO; GO:0051496; P:positive regulation of stress fiber assembly; ISO:MGI.
DR GO; GO:0032224; P:positive regulation of synaptic transmission, cholinergic; ISO:MGI.
DR GO; GO:0032230; P:positive regulation of synaptic transmission, GABAergic; ISO:MGI.
DR GO; GO:0008217; P:regulation of blood pressure; ISO:MGI.
DR GO; GO:0051930; P:regulation of sensory perception of pain; ISO:MGI.
DR GO; GO:0009725; P:response to hormone; ISO:MGI.
DR GO; GO:0048265; P:response to pain; IMP:MGI.
DR GO; GO:0001878; P:response to yeast; ISO:MGI.
DR GO; GO:0019233; P:sensory perception of pain; IMP:MGI.
DR GO; GO:0007217; P:tachykinin receptor signaling pathway; ISO:MGI.
DR InterPro; IPR013055; Tachy_Neuro_lke_CS.
DR InterPro; IPR008215; Tachykinin_dom.
DR InterPro; IPR008216; Tachykinin_fam.
DR Pfam; PF02202; Tachykinin; 1.
DR PRINTS; PR01829; PROTACHYKNIN.
DR SMART; SM00203; TK; 2.
DR PROSITE; PS00267; TACHYKININ; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amidation; Cleavage on pair of basic residues;
KW Neuropeptide; Neurotransmitter; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..56
FT /evidence="ECO:0000255"
FT /id="PRO_0000033543"
FT PEPTIDE 58..68
FT /note="Substance P"
FT /id="PRO_0000033544"
FT PEPTIDE 72..107
FT /note="Neuropeptide K"
FT /id="PRO_0000033545"
FT PEPTIDE 72..73
FT /note="Neuropeptide gamma, 1st part"
FT /id="PRO_0000033546"
FT PEPTIDE 89..107
FT /note="Neuropeptide gamma, 2nd part"
FT /id="PRO_0000033547"
FT PEPTIDE 98..107
FT /note="Neurokinin A"
FT /id="PRO_0000033548"
FT PEPTIDE 111..126
FT /note="C-terminal-flanking peptide"
FT /evidence="ECO:0000250"
FT /id="PRO_0000033549"
FT SITE 59..60
FT /note="Cleavage; by FAP"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT SITE 63..64
FT /note="Cleavage; by MME"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT SITE 64..65
FT /note="Cleavage; by MME"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT SITE 65..66
FT /note="Cleavage; by ACE"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT SITE 66..67
FT /note="Cleavage; by ACE and MME"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT MOD_RES 68
FT /note="Methionine amide"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT MOD_RES 107
FT /note="Methionine amide"
FT /evidence="ECO:0000250|UniProtKB:P20366"
FT VAR_SEQ 74..88
FT /note="Missing (in isoform Gamma)"
FT /evidence="ECO:0000303|PubMed:1708336"
FT /id="VSP_006379"
SQ SEQUENCE 130 AA; 15045 MW; 7BE8DA15FDE72FF8 CRC64;
MKILVAVAVF FLVSTQLFAE EIDANDDLNY WSDWSDSDQI KEAMPEPFEH LLQRIARRPK
PQQFFGLMGK RDADSSVEKQ VALLKALYGH GQISHKRHKT DSFVGLMGKR ALNSVAYERS
AMQNYERRRK
