ID   BSN1_BACAM              Reviewed;         289 AA.
AC   Q03091;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Extracellular ribonuclease;
DE            EC=3.1.-.-;
DE   Flags: Precursor;
GN   Name=bsn;
OS   Bacillus amyloliquefaciens (Bacillus velezensis).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus amyloliquefaciens group.
OX   NCBI_TaxID=1390;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 52-63.
RC   STRAIN=NBRC 3034 / 306;
RX   PubMed=1396690; DOI=10.1111/j.1432-1033.1992.tb17268.x;
RA   Nakamura A., Koide Y., Miyazaki H., Kitamura A., Masaki H., Beppu T.,
RA   Uozumi T.;
RT   "Gene cloning and characterization of a novel extracellular ribonuclease of
RT   Bacillus subtilis.";
RL   Eur. J. Biochem. 209:121-127(1992).
CC   -!- FUNCTION: Mg(2+)-activated ribonuclease which hydrolyzes RNA apparently
CC       nonspecifically into oligonucleotides with 5'-terminal phosphate.
CC   -!- SUBCELLULAR LOCATION: Secreted.
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DR   EMBL; D01097; BAA00882.1; -; Genomic_DNA.
DR   PIR; S29271; S29271.
DR   RefSeq; WP_003151902.1; NZ_WOYD01000002.1.
DR   AlphaFoldDB; Q03091; -.
DR   STRING; 692420.BAMF_3049; -.
DR   GeneID; 66325054; -.
DR   eggNOG; COG2356; Bacteria.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR007346; Endonuclease-I.
DR   InterPro; IPR044925; His-Me_finger_sf.
DR   PANTHER; PTHR33607; PTHR33607; 1.
DR   Pfam; PF04231; Endonuclease_1; 1.
DR   SUPFAM; SSF54060; SSF54060; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Nuclease; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..51
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000020830"
FT   CHAIN           52..289
FT                   /note="Extracellular ribonuclease"
FT                   /id="PRO_0000020831"
FT   REGION          177..197
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   289 AA;  32027 MW;  40CDB6CFDB0BC69C CRC64;
     MTKKLWFLPI VCLFFILGWT APSASAGAPA DTNLYSRLAV STAGGTTLFP QTSSAVITPS
     ADTETYYKEA SGKSGTALKS ALHRIISGHT KLSYSQVWNA LKETDEDPAN PNNVILLYTQ
     ESRAKSKNGG SVGDWNREHV WAKSHGNFGT AAGPGTDIHH LRPADVQVNS ARGNMDFDNG
     GSEYPKAPGN YYDGDSWEPR DEVKGDVARM LFYMAVRYEG GDGYPDLELN DKTGNGSAPY
     MGKLSVLLKW NKQDPVDSKE KRRNEIIYED YQHNRNPFID HPEWADEIW