ID   BSN2_BACSU              Reviewed;         288 AA.
AC   O32150;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Extracellular ribonuclease;
DE            EC=3.1.-.-;
DE   Flags: Precursor;
GN   Name=bsn; Synonyms=yurI; OrderedLocusNames=BSU32540;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: Mg(2+)-activated ribonuclease which hydrolyzes RNA apparently
CC       nonspecifically into oligonucleotides with 5'-terminal phosphate.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
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DR   EMBL; AL009126; CAB15244.1; -; Genomic_DNA.
DR   PIR; H70017; H70017.
DR   RefSeq; NP_391134.1; NC_000964.3.
DR   RefSeq; WP_009968119.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O32150; -.
DR   STRING; 224308.BSU32540; -.
DR   PaxDb; O32150; -.
DR   PRIDE; O32150; -.
DR   EnsemblBacteria; CAB15244; CAB15244; BSU_32540.
DR   GeneID; 936687; -.
DR   KEGG; bsu:BSU32540; -.
DR   PATRIC; fig|224308.179.peg.3524; -.
DR   eggNOG; COG2356; Bacteria.
DR   InParanoid; O32150; -.
DR   OMA; RNQVIYD; -.
DR   PhylomeDB; O32150; -.
DR   BioCyc; BSUB:BSU32540-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR007346; Endonuclease-I.
DR   InterPro; IPR044925; His-Me_finger_sf.
DR   PANTHER; PTHR33607; PTHR33607; 1.
DR   Pfam; PF04231; Endonuclease_1; 1.
DR   SUPFAM; SSF54060; SSF54060; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Nuclease; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..288
FT                   /note="Extracellular ribonuclease"
FT                   /id="PRO_0000020832"
SQ   SEQUENCE   288 AA;  32094 MW;  F884777DA27FE8F7 CRC64;
     MTKKAWFLPL VCVLLISGWL APAASASAQT TLSLNDRLAS SPSGTGSLLS LAAPAAPYAD
     TDTYYEGAEG KTGDSLKSTL HRIISGHTML SYSEVWNALK ETDEDPRNPN NVILLYTNES
     RSKNLNGGNV GDWNREHVWA KSHGDFGTSK GPGTDIHHLR PADVQVNSAR GNMDFDNGGT
     EYAKAPGNYY DGDSWEPRDD VKGDVARMLF YMAVRYEGDD GYPDLELNDK TGNGSAPYHG
     KQSVLLEWNK QDPVDDRERK RNEIIYEKYQ HNRNPFIDHP EWADEIWP