TL15A_ARATH
ID TL15A_ARATH Reviewed; 224 AA.
AC O22160; Q53YH9; Q949R8;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 25-MAY-2022, entry version 140.
DE RecName: Full=Thylakoid lumenal 15 kDa protein 1, chloroplastic;
DE AltName: Full=p15;
DE Flags: Precursor;
GN OrderedLocusNames=At2g44920; ORFNames=T13E15.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP PROTEIN SEQUENCE OF 82-101, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=11719511; DOI=10.1074/jbc.m108575200;
RA Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P.,
RA Kieselbach T.;
RT "Proteome map of the chloroplast lumen of Arabidopsis thaliana.";
RL J. Biol. Chem. 277:8354-8365(2002).
CC -!- INTERACTION:
CC O22160; P0AA25: trxA; Xeno; NbExp=2; IntAct=EBI-2895776, EBI-368542;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen
CC {ECO:0000269|PubMed:11719511}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O22160-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O22160-2; Sequence=VSP_004655;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC002388; AAM14836.1; -; Genomic_DNA.
DR EMBL; AC002388; AAC31832.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC10484.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10485.1; -; Genomic_DNA.
DR EMBL; AY050941; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BT000902; AAN41302.1; -; mRNA.
DR PIR; T00401; T00401.
DR RefSeq; NP_566030.1; NM_130056.6. [O22160-1]
DR RefSeq; NP_566031.1; NM_130057.4. [O22160-2]
DR PDB; 3N90; X-ray; 1.70 A; A=81-224.
DR PDBsum; 3N90; -.
DR AlphaFoldDB; O22160; -.
DR SMR; O22160; -.
DR IntAct; O22160; 1.
DR STRING; 3702.AT2G44920.2; -.
DR PaxDb; O22160; -.
DR PRIDE; O22160; -.
DR ProteomicsDB; 232459; -. [O22160-1]
DR EnsemblPlants; AT2G44920.1; AT2G44920.1; AT2G44920. [O22160-2]
DR EnsemblPlants; AT2G44920.2; AT2G44920.2; AT2G44920. [O22160-1]
DR GeneID; 819101; -.
DR Gramene; AT2G44920.1; AT2G44920.1; AT2G44920. [O22160-2]
DR Gramene; AT2G44920.2; AT2G44920.2; AT2G44920. [O22160-1]
DR KEGG; ath:AT2G44920; -.
DR Araport; AT2G44920; -.
DR TAIR; locus:2055023; AT2G44920.
DR eggNOG; ENOG502QQA1; Eukaryota.
DR HOGENOM; CLU_066336_1_0_1; -.
DR InParanoid; O22160; -.
DR OMA; DQRNLCK; -.
DR PhylomeDB; O22160; -.
DR PRO; PR:O22160; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22160; baseline and differential.
DR Genevisible; O22160; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009543; C:chloroplast thylakoid lumen; IDA:UniProtKB.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0031977; C:thylakoid lumen; HDA:TAIR.
DR InterPro; IPR001646; 5peptide_repeat.
DR InterPro; IPR044213; At2g44920-like.
DR PANTHER; PTHR47200; PTHR47200; 1.
DR Pfam; PF00805; Pentapeptide; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Chloroplast; Direct protein sequencing;
KW Plastid; Reference proteome; Repeat; Thylakoid; Transit peptide.
FT TRANSIT 1..34
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 35..81
FT /note="Thylakoid"
FT /evidence="ECO:0000269|PubMed:11719511"
FT CHAIN 82..224
FT /note="Thylakoid lumenal 15 kDa protein 1, chloroplastic"
FT /id="PRO_0000023523"
FT DOMAIN 116..155
FT /note="Pentapeptide repeat 1"
FT DOMAIN 156..196
FT /note="Pentapeptide repeat 2"
FT VAR_SEQ 188..224
FT /note="DFTDVPLRDDQRVYLCKVADGVNATTGNATRDTLLCN -> GEKRKENFHNN
FT NKHISLLPLSFT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_004655"
FT HELIX 82..85
FT /evidence="ECO:0007829|PDB:3N90"
FT HELIX 90..93
FT /evidence="ECO:0007829|PDB:3N90"
FT HELIX 196..205
FT /evidence="ECO:0007829|PDB:3N90"
FT TURN 211..213
FT /evidence="ECO:0007829|PDB:3N90"
FT HELIX 217..220
FT /evidence="ECO:0007829|PDB:3N90"
FT TURN 221..224
FT /evidence="ECO:0007829|PDB:3N90"
SQ SEQUENCE 224 AA; 23779 MW; ABCECC58F04ADEE4 CRC64;
MVILSNVSLF SCCNISQKPS LFSPSSRSSH CPIRCSQSQE GKEVVTSPLR SVVWSLGEEV
SKRSLFALVS ASLFFVDPAL AFKGGGPYGQ GVTRGQDLSG KDFSGQTLIR QDFKTSILRQ
ANFKGAKLLG ASFFDADLTG ADLSEADLRG ADFSLANVTK VNLTNANLEG ATVTGNTSFK
GSNITGADFT DVPLRDDQRV YLCKVADGVN ATTGNATRDT LLCN
