TL17_ARATH
ID TL17_ARATH Reviewed; 236 AA.
AC P81760; Q3E8C0; Q93Y82;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 21-NOV-2003, sequence version 2.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Thylakoid lumenal 17.4 kDa protein, chloroplastic {ECO:0000303|PubMed:9654141};
DE AltName: Full=P17.4 {ECO:0000303|PubMed:9654141};
DE Flags: Precursor;
GN Name=TL17 {ECO:0000303|PubMed:11719511};
GN OrderedLocusNames=At5g53490 {ECO:0000312|Araport:AT5G53490};
GN ORFNames=MNC6.3 {ECO:0000312|EMBL:BAB09725.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=9654141; DOI=10.1016/s0014-5793(98)00517-1;
RA Kieselbach T., Mant A., Robinson C., Schroeder W.P.;
RT "Characterisation of an Arabidopsis cDNA encoding a thylakoid lumen protein
RT related to a novel 'pentapeptide repeat' family of proteins.";
RL FEBS Lett. 428:241-244(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP PROTEIN SEQUENCE OF 78-91, AND SUBCELLULAR LOCATION.
RX PubMed=11719511; DOI=10.1074/jbc.m108575200;
RA Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P.,
RA Kieselbach T.;
RT "Proteome map of the chloroplast lumen of Arabidopsis thaliana.";
RL J. Biol. Chem. 277:8354-8365(2002).
RN [6]
RP PROTEIN SEQUENCE OF N-TERMINUS, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11826309; DOI=10.1105/tpc.010304;
RA Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G.,
RA Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G.,
RA van Wijk K.J.;
RT "Central functions of the lumenal and peripheral thylakoid proteome of
RT Arabidopsis determined by experimentation and genome-wide prediction.";
RL Plant Cell 14:211-236(2002).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [8]
RP INTERACTION WITH LTO1.
RX PubMed=25412899; DOI=10.2174/0929866521666141121153138;
RA Lu Y., Du J.J., Yu Z.B., Peng J.J., Xu J.N., Wang X.Y.;
RT "Identification of potential targets for thylakoid oxidoreductase
RT AtVKOR/LTO1 in chloroplasts.";
RL Protein Pept. Lett. 22:219-225(2014).
CC -!- SUBUNIT: Interacts in vitro with LTO1. {ECO:0000269|PubMed:25412899}.
CC -!- INTERACTION:
CC P81760; P0AA25: trxA; Xeno; NbExp=2; IntAct=EBI-449573, EBI-368542;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen
CC {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:18431481}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P81760-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P81760-2; Sequence=VSP_034389;
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DR EMBL; AB015476; BAB09725.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96367.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96368.1; -; Genomic_DNA.
DR EMBL; AF370552; AAK48979.1; -; mRNA.
DR EMBL; BT003410; AAO30073.1; -; mRNA.
DR RefSeq; NP_200161.2; NM_124729.4. [P81760-2]
DR RefSeq; NP_851183.1; NM_180852.3. [P81760-1]
DR AlphaFoldDB; P81760; -.
DR SMR; P81760; -.
DR BioGRID; 20675; 1.
DR IntAct; P81760; 2.
DR STRING; 3702.AT5G53490.3; -.
DR MetOSite; P81760; -.
DR EnsemblPlants; AT5G53490.1; AT5G53490.1; AT5G53490. [P81760-1]
DR EnsemblPlants; AT5G53490.2; AT5G53490.2; AT5G53490. [P81760-2]
DR GeneID; 835431; -.
DR Gramene; AT5G53490.1; AT5G53490.1; AT5G53490. [P81760-1]
DR Gramene; AT5G53490.2; AT5G53490.2; AT5G53490. [P81760-2]
DR KEGG; ath:AT5G53490; -.
DR Araport; AT5G53490; -.
DR eggNOG; ENOG502QTR1; Eukaryota.
DR HOGENOM; CLU_066336_0_0_1; -.
DR InParanoid; P81760; -.
DR PhylomeDB; P81760; -.
DR PRO; PR:P81760; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P81760; baseline and differential.
DR Genevisible; P81760; AT.
DR GO; GO:0009543; C:chloroplast thylakoid lumen; IEA:UniProtKB-SubCell.
DR InterPro; IPR001646; 5peptide_repeat.
DR InterPro; IPR044195; TL17-like.
DR PANTHER; PTHR47485; PTHR47485; 1.
DR Pfam; PF13599; Pentapeptide_4; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Direct protein sequencing; Plastid;
KW Reference proteome; Repeat; Thylakoid; Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT ?..77
FT /note="Thylakoid"
FT /evidence="ECO:0000269|PubMed:11719511,
FT ECO:0000269|PubMed:11826309"
FT CHAIN 78..236
FT /note="Thylakoid lumenal 17.4 kDa protein, chloroplastic"
FT /id="PRO_0000023524"
FT DOMAIN 124..163
FT /note="Pentapeptide repeat 1"
FT DOMAIN 169..208
FT /note="Pentapeptide repeat 2"
FT VAR_SEQ 36
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_034389"
FT CONFLICT 21
FT /note="N -> K (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 236 AA; 25644 MW; D14502C4372A3A14 CRC64;
MASLPVQFTR NQISSPFFSV NLRREPRSLV TVHCSAGENR ENGEGVKKSL FPLKELGSIA
CAALCACTLT IASPVIAANQ RLPPLSTEPD RCEKAFVGNT IGQANGVYDK PLDLRFCDYT
NDQTNLKGKT LSAALMVGAK FDGADMTEVV MSKAYAVEAS FKGVNFTNAV IDRVNFGKSN
LKGAVFRNTV LSGSTFEEAN LEDVVFEDTI IGYIDLQKIC RNESINEEGR LVLGCR
