TL203_ARATH
ID TL203_ARATH Reviewed; 280 AA.
AC Q8H1Q1; Q94C72; Q9FWX1;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Thylakoid lumenal protein TL20.3, chloroplastic {ECO:0000303|PubMed:20049866};
DE Flags: Precursor;
GN Name=TL20.3 {ECO:0000303|PubMed:20049866};
GN OrderedLocusNames=At1g12250 {ECO:0000312|Araport:AT1G12250};
GN ORFNames=T28K15.2 {ECO:0000312|EMBL:AAG12570.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH THIOREDOXIN.
RX PubMed=20049866; DOI=10.1002/pmic.200900654;
RA Hall M., Mata-Cabana A., Akerlund H.E., Florencio F.J., Schroeder W.P.,
RA Lindahl M., Kieselbach T.;
RT "Thioredoxin targets of the plant chloroplast lumen and their implications
RT for plastid function.";
RL Proteomics 10:987-1001(2010).
RN [6]
RP INTERACTION WITH LTO1.
RX PubMed=25412899; DOI=10.2174/0929866521666141121153138;
RA Lu Y., Du J.J., Yu Z.B., Peng J.J., Xu J.N., Wang X.Y.;
RT "Identification of potential targets for thylakoid oxidoreductase
RT AtVKOR/LTO1 in chloroplasts.";
RL Protein Pept. Lett. 22:219-225(2014).
CC -!- FUNCTION: Pentapeptide repeat protein of unknown function. Subject to
CC degradation when reduced. {ECO:0000269|PubMed:20049866}.
CC -!- SUBUNIT: Interacts with thioredoxin (PubMed:20049866). Interacts in
CC vitro with LTO1 (PubMed:25412899). {ECO:0000269|PubMed:20049866,
CC ECO:0000269|PubMed:25412899}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen
CC {ECO:0000269|PubMed:18431481, ECO:0000269|PubMed:20049866}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8H1Q1-1; Sequence=Displayed;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG12570.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC022522; AAG12570.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28857.1; -; Genomic_DNA.
DR EMBL; AY142640; AAN13098.1; -; mRNA.
DR EMBL; AY035122; AAK59627.1; -; mRNA.
DR PIR; F86257; F86257.
DR RefSeq; NP_563902.1; NM_101097.4. [Q8H1Q1-1]
DR AlphaFoldDB; Q8H1Q1; -.
DR SMR; Q8H1Q1; -.
DR BioGRID; 23018; 1.
DR IntAct; Q8H1Q1; 1.
DR STRING; 3702.AT1G12250.1; -.
DR PaxDb; Q8H1Q1; -.
DR PRIDE; Q8H1Q1; -.
DR EnsemblPlants; AT1G12250.1; AT1G12250.1; AT1G12250. [Q8H1Q1-1]
DR GeneID; 837778; -.
DR Gramene; AT1G12250.1; AT1G12250.1; AT1G12250. [Q8H1Q1-1]
DR KEGG; ath:AT1G12250; -.
DR Araport; AT1G12250; -.
DR TAIR; locus:2202033; AT1G12250.
DR eggNOG; ENOG502QSEG; Eukaryota.
DR HOGENOM; CLU_066336_4_0_1; -.
DR InParanoid; Q8H1Q1; -.
DR OrthoDB; 1288165at2759; -.
DR PhylomeDB; Q8H1Q1; -.
DR PRO; PR:Q8H1Q1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8H1Q1; baseline and differential.
DR Genevisible; Q8H1Q1; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009543; C:chloroplast thylakoid lumen; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR InterPro; IPR001646; 5peptide_repeat.
DR Pfam; PF00805; Pentapeptide; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Plastid; Reference proteome; Repeat;
KW Thylakoid; Transit peptide.
FT TRANSIT 1..59
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 60..90
FT /note="Thylakoid"
FT /evidence="ECO:0000255"
FT CHAIN 91..280
FT /note="Thylakoid lumenal protein TL20.3, chloroplastic"
FT /id="PRO_0000342096"
FT DOMAIN 124..163
FT /note="Pentapeptide repeat 1"
FT /evidence="ECO:0000305"
FT DOMAIN 169..208
FT /note="Pentapeptide repeat 2"
FT /evidence="ECO:0000305"
FT CONFLICT 66
FT /note="T -> K (in Ref. 3; AAK59627)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 280 AA; 30065 MW; 81CA7C8428ADBF1D CRC64;
MAFSSLSPLP MKSLDISRSS SSVSRSPYHF QRYLLRRLQL SSRSNLEIKD SSNTREGCCS
SAESNTWKRI LSAAMAAAVI ASSSGVPAMA ELNRFEADTR GEFGIGSAAQ YGSADLSKTV
HSNENFRRAN FTSADMRESD FSGSTFNGAY LEKAVAYKAN FSGADLSDTL MDRMVLNEAN
LTNAVLVRSV LTRSDLGGAK IEGADFSDAV IDLLQKQALC KYATGTNPLT GVDTRKSLGC
GNSRRNAYGS PSSPLLSAPP QRLLGRDGFC DEKTGLCDVK
