TLC1_ARATH
ID TLC1_ARATH Reviewed; 624 AA.
AC Q39002; Q94AN9; Q9C974;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 27-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=ADP,ATP carrier protein 1, chloroplastic;
DE AltName: Full=ADP/ATP translocase 1;
DE AltName: Full=Adenine nucleotide translocase 1;
DE Flags: Precursor;
GN Name=AATP1; OrderedLocusNames=At1g80300; ORFNames=F5I6.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RX PubMed=7589569; DOI=10.1016/0014-5793(95)01143-3;
RA Kampfenkel K., Moehlmann T., Batz O., van Montagu M., Inze D.,
RA Neuhaus H.E.;
RT "Molecular characterization of an Arabidopsis thaliana cDNA encoding a
RT novel putative adenylate translocator of higher plants.";
RL FEBS Lett. 374:351-355(1995).
RN [2]
RP SEQUENCE REVISION.
RA Kampfenkel K.;
RL Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=9025303; DOI=10.1046/j.1365-313x.1997.11010073.x;
RA Neuhaus H.E., Thom E., Mohlmann T., Steup M., Kampfenkel K.;
RT "Characterization of a novel eukaryotic ATP/ADP translocator located in the
RT plastid envelope of Arabidopsis thaliana L.";
RL Plant J. 11:73-82(1997).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-80, CLEAVAGE OF TRANSIT PEPTIDE
RP [LARGE SCALE ANALYSIS] AFTER LYS-79, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: May function as an ATP importer.
CC {ECO:0000269|PubMed:9025303}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:9025303}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:9025303}.
CC -!- SIMILARITY: Belongs to the ADP/ATP translocase tlc (TC 2.A.12.2)
CC family. {ECO:0000305}.
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DR EMBL; Z49227; CAA89201.2; -; mRNA.
DR EMBL; AC018848; AAG52434.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36385.1; -; Genomic_DNA.
DR EMBL; AF428316; AAL16246.1; -; mRNA.
DR EMBL; AY045903; AAK76577.1; -; mRNA.
DR PIR; E96834; E96834.
DR PIR; S68205; S68205.
DR RefSeq; NP_178146.1; NM_106679.3.
DR AlphaFoldDB; Q39002; -.
DR BioGRID; 29588; 13.
DR IntAct; Q39002; 13.
DR STRING; 3702.AT1G80300.1; -.
DR TCDB; 2.A.12.1.16; the atp:adp antiporter (aaa) family.
DR iPTMnet; Q39002; -.
DR PaxDb; Q39002; -.
DR PRIDE; Q39002; -.
DR ProteomicsDB; 246428; -.
DR EnsemblPlants; AT1G80300.1; AT1G80300.1; AT1G80300.
DR GeneID; 844370; -.
DR Gramene; AT1G80300.1; AT1G80300.1; AT1G80300.
DR KEGG; ath:AT1G80300; -.
DR Araport; AT1G80300; -.
DR TAIR; locus:2034235; AT1G80300.
DR eggNOG; ENOG502QSRY; Eukaryota.
DR HOGENOM; CLU_023964_0_0_1; -.
DR InParanoid; Q39002; -.
DR OMA; RKVIWPI; -.
DR OrthoDB; 1270398at2759; -.
DR PhylomeDB; Q39002; -.
DR BioCyc; MetaCyc:AT1G80300-MON; -.
DR PRO; PR:Q39002; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q39002; baseline and differential.
DR Genevisible; Q39002; AT.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005471; F:ATP:ADP antiporter activity; IDA:TAIR.
DR InterPro; IPR004667; ADP_ATP_car_bac_type.
DR PANTHER; PTHR31187; PTHR31187; 1.
DR Pfam; PF03219; TLC; 1.
DR TIGRFAMs; TIGR00769; AAA; 1.
PE 1: Evidence at protein level;
KW Acetylation; ATP-binding; Chloroplast; Membrane; Nucleotide-binding;
KW Plastid; Reference proteome; Transit peptide; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..79
FT /note="Chloroplast"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 80..624
FT /note="ADP,ATP carrier protein 1, chloroplastic"
FT /id="PRO_0000034359"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 446..466
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 545..565
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 579..624
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 596..624
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 80
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CONFLICT 45..94
FT /note="LSLSFNGHKKFQTFEPTLHGISISHKERSTEFICKAEAAAAGDGAVFGEG
FT -> CLYPLTGTRNFKPLSQPCMGFRFPTKREAPSSYARRRRGCWRRSCLRRS (in
FT Ref. 1; CAA89201)"
FT /evidence="ECO:0000305"
FT CONFLICT 103
FT /note="P -> R (in Ref. 1; CAA89201)"
FT /evidence="ECO:0000305"
FT CONFLICT 197
FT /note="A -> G (in Ref. 1; CAA89201)"
FT /evidence="ECO:0000305"
FT CONFLICT 281
FT /note="A -> G (in Ref. 5; AAK76577)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 624 AA; 68134 MW; 530302A89AD08745 CRC64;
MEAVIQTRGL LSLPTKPIGV RSQLQPSHGL KQRLFAAKPR NLHGLSLSFN GHKKFQTFEP
TLHGISISHK ERSTEFICKA EAAAAGDGAV FGEGDSAAVV ASPKIFGVEV ATLKKIIPLG
LMFFCILFNY TILRDTKDVL VVTAKGSSAE IIPFLKTWVN LPMAIGFMLL YTKLSNVLSK
KALFYTVIVP FIIYFGAFGF VMYPLSNYIH PEALADKLLT TLGPRFMGPI AILRIWSFCL
FYVMAELWGS VVVSVLFWGF ANQITTVDEA KKFYPLFGLG ANVALIFSGR TVKYFSNLRK
NLGPGVDGWA VSLKAMMSIV VGMGLAICLL YWWVNRYVPL PTRSKNKKEK PKMGTMESLK
FLVSSPYIRD LATLVVAYGI SINLVEVTWK SKLKAQFPSP NEYSAFMGDF STCTGVATFT
MMLLSQYVFN KYGWGVAAKI TPTVLLLTGV AFFSLILFGG PFAPLVAKLG MTPLLAAVYV
GALQNIFSKS AKYSLFDPCK EMAYIPLDED TKVKGKAAID VVCNPLGKSG GALIQQFMIL
SFGSLANSTP YLGMILLVIV TAWLAAAKSL EGQFNSLRSE EELEKEMERA SSVKIPVVSQ
DESGNGSLGE SPSSSPEKSA PTNL
