TLCD1_MOUSE
ID TLCD1_MOUSE Reviewed; 247 AA.
AC Q99JT6; Q3V372; Q5SYM9; Q8CHT9; Q9DCK5;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=TLC domain-containing protein 1;
DE AltName: Full=Calfacilitin;
DE Flags: Precursor;
GN Name=Tlcd1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
RC STRAIN=C57BL/6J; TISSUE=Adipose tissue, Kidney, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=Czech II, and FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Regulates the composition and fluidity of the plasma membrane
CC (By similarity). Inhibits the incorporation of membrane-fluidizing
CC phospholipids containing omega-3 long-chain polyunsaturated fatty acids
CC (LCPUFA) and thereby promotes membrane rigidity (By similarity). Does
CC not appear to have any effect on LCPUFA synthesis (By similarity).
CC {ECO:0000250|UniProtKB:Q96CP7}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q96CP7};
CC Multi-pass membrane protein {ECO:0000250|UniProtKB:F1NZP5}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q99JT6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99JT6-2; Sequence=VSP_024890;
CC Name=3;
CC IsoId=Q99JT6-3; Sequence=VSP_024887;
CC Name=4;
CC IsoId=Q99JT6-4; Sequence=VSP_024888, VSP_024889;
CC -!- CAUTION: Was originally proposed to be a calcium channel facilitator
CC (By similarity). However, a more recent study shows that this protein
CC regulates membrane phospholipid homeostasis (By similarity). Therefore,
CC any effects on calcium flux are most likely a secondary consequence of
CC defects in membrane composition or fluidity (By similarity).
CC {ECO:0000250|UniProtKB:F1NZP5, ECO:0000250|UniProtKB:Q96CP7}.
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DR EMBL; AK002703; BAB22297.1; -; mRNA.
DR EMBL; AK046577; BAE20654.1; -; mRNA.
DR EMBL; AK161721; BAE36548.1; -; mRNA.
DR EMBL; AK165432; BAE38185.1; -; mRNA.
DR EMBL; AL591070; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC005702; AAH05702.1; -; mRNA.
DR EMBL; BC039182; AAH39182.1; -; mRNA.
DR CCDS; CCDS25092.1; -. [Q99JT6-1]
DR CCDS; CCDS70257.1; -. [Q99JT6-3]
DR RefSeq; NP_001278165.1; NM_001291236.1. [Q99JT6-3]
DR RefSeq; NP_001278166.1; NM_001291237.1. [Q99JT6-3]
DR RefSeq; NP_080984.1; NM_026708.2. [Q99JT6-1]
DR RefSeq; XP_006534117.1; XM_006534054.2. [Q99JT6-1]
DR AlphaFoldDB; Q99JT6; -.
DR BioGRID; 212831; 2.
DR STRING; 10090.ENSMUSP00000114202; -.
DR PhosphoSitePlus; Q99JT6; -.
DR MaxQB; Q99JT6; -.
DR PaxDb; Q99JT6; -.
DR PRIDE; Q99JT6; -.
DR ProteomicsDB; 259397; -. [Q99JT6-1]
DR ProteomicsDB; 259398; -. [Q99JT6-2]
DR ProteomicsDB; 259399; -. [Q99JT6-3]
DR ProteomicsDB; 259400; -. [Q99JT6-4]
DR Antibodypedia; 51370; 72 antibodies from 14 providers.
DR Ensembl; ENSMUST00000092880; ENSMUSP00000090556; ENSMUSG00000019437. [Q99JT6-3]
DR Ensembl; ENSMUST00000098545; ENSMUSP00000096145; ENSMUSG00000019437. [Q99JT6-2]
DR Ensembl; ENSMUST00000108338; ENSMUSP00000103975; ENSMUSG00000019437. [Q99JT6-4]
DR Ensembl; ENSMUST00000127587; ENSMUSP00000114202; ENSMUSG00000019437. [Q99JT6-1]
DR GeneID; 68385; -.
DR KEGG; mmu:68385; -.
DR UCSC; uc007kij.2; mouse. [Q99JT6-1]
DR UCSC; uc007kik.2; mouse. [Q99JT6-3]
DR CTD; 116238; -.
DR MGI; MGI:1915572; Tlcd1.
DR VEuPathDB; HostDB:ENSMUSG00000019437; -.
DR eggNOG; KOG4474; Eukaryota.
DR GeneTree; ENSGT01010000222313; -.
DR HOGENOM; CLU_056440_2_0_1; -.
DR InParanoid; Q99JT6; -.
DR OMA; CAGQNGM; -.
DR OrthoDB; 1113854at2759; -.
DR PhylomeDB; Q99JT6; -.
DR TreeFam; TF315115; -.
DR BioGRID-ORCS; 68385; 8 hits in 75 CRISPR screens.
DR PRO; PR:Q99JT6; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q99JT6; protein.
DR Bgee; ENSMUSG00000019437; Expressed in yolk sac and 228 other tissues.
DR ExpressionAtlas; Q99JT6; baseline and differential.
DR Genevisible; Q99JT6; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0055088; P:lipid homeostasis; IBA:GO_Central.
DR GO; GO:0071709; P:membrane assembly; ISO:MGI.
DR GO; GO:0055091; P:phospholipid homeostasis; ISO:MGI.
DR GO; GO:0007009; P:plasma membrane organization; ISO:MGI.
DR GO; GO:0097035; P:regulation of membrane lipid distribution; ISO:MGI.
DR InterPro; IPR006634; TLC-dom.
DR Pfam; PF03798; TRAM_LAG1_CLN8; 1.
DR SMART; SM00724; TLC; 1.
DR PROSITE; PS50922; TLC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..247
FT /note="TLC domain-containing protein 1"
FT /id="PRO_0000285678"
FT TOPO_DOM 28..46
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..83
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..123
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT INTRAMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..173
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..201
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..247
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT DOMAIN 40..234
FT /note="TLC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00205"
FT VAR_SEQ 1..65
FT /note="MPLLFHPAWPLLLGATLTFRALRRVLCRLPQPAHVQTDPLRTWRWHNLLVSF
FT THSIVSGIWALLC -> MRWPWDLSRGARTGLPLAIPITGEQTPRISPDSVWKTTQPRT
FT AVGNPS (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_024887"
FT VAR_SEQ 121..127
FT /note="AMGAFFS -> VRENPVK (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024888"
FT VAR_SEQ 128..247
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024889"
FT VAR_SEQ 170..196
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024890"
FT CONFLICT 28
FT /note="R -> H (in Ref. 1; BAE20654)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 247 AA; 28787 MW; 84AEA3CBE8BE4093 CRC64;
MPLLFHPAWP LLLGATLTFR ALRRVLCRLP QPAHVQTDPL RTWRWHNLLV SFTHSIVSGI
WALLCLWQTP EMLVEIETAW SASGYLLVCF SAGYFIHDTV DIVVSKQTRA SWEYLVHHVM
AMGAFFSGIF WKRFVGGGVL TLLVEVSNIF LTLRMMMKIN NAQDLLLYKV NKYINLVMYF
LFRLAPQAYL TKFFLQYAGQ RTLGTFLLAI LLMLDLMIII YFSRLLRSDF CPERAPRRQQ
KDKFLTE
