ID   TLCD1_RAT               Reviewed;         247 AA.
AC   Q5U2T1;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=TLC domain-containing protein 1;
DE   AltName: Full=Calfacilitin;
DE   Flags: Precursor;
GN   Name=Tlcd1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Regulates the composition and fluidity of the plasma membrane
CC       (By similarity). Inhibits the incorporation of membrane-fluidizing
CC       phospholipids containing omega-3 long-chain polyunsaturated fatty acids
CC       (LCPUFA) and thereby promotes membrane rigidity (By similarity). Does
CC       not appear to have any effect on LCPUFA synthesis (By similarity).
CC       {ECO:0000250|UniProtKB:Q96CP7}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q96CP7};
CC       Multi-pass membrane protein {ECO:0000250|UniProtKB:F1NZP5}.
CC   -!- CAUTION: Was originally proposed to be a calcium channel facilitator
CC       (By similarity). However, a more recent study shows that this protein
CC       regulates membrane phospholipid homeostasis (By similarity). Therefore,
CC       any effects on calcium flux are most likely a secondary consequence of
CC       defects in membrane composition or fluidity (By similarity).
CC       {ECO:0000250|UniProtKB:F1NZP5, ECO:0000250|UniProtKB:Q96CP7}.
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DR   EMBL; BC085876; AAH85876.1; -; mRNA.
DR   RefSeq; NP_001013880.1; NM_001013858.1.
DR   AlphaFoldDB; Q5U2T1; -.
DR   STRING; 10116.ENSRNOP00000016840; -.
DR   PaxDb; Q5U2T1; -.
DR   PRIDE; Q5U2T1; -.
DR   Ensembl; ENSRNOT00000098225; ENSRNOP00000084592; ENSRNOG00000071015.
DR   GeneID; 287472; -.
DR   KEGG; rno:287472; -.
DR   CTD; 116238; -.
DR   RGD; 1359195; Tlcd1.
DR   eggNOG; KOG4474; Eukaryota.
DR   GeneTree; ENSGT01010000222313; -.
DR   HOGENOM; CLU_056440_2_1_1; -.
DR   InParanoid; Q5U2T1; -.
DR   OMA; CAGQNGM; -.
DR   OrthoDB; 1113854at2759; -.
DR   PhylomeDB; Q5U2T1; -.
DR   TreeFam; TF315115; -.
DR   PRO; PR:Q5U2T1; -.
DR   Proteomes; UP000002494; Chromosome 10.
DR   Bgee; ENSRNOG00000012579; Expressed in pancreas and 20 other tissues.
DR   Genevisible; Q5U2T1; RN.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR   GO; GO:0055088; P:lipid homeostasis; IBA:GO_Central.
DR   GO; GO:0071709; P:membrane assembly; ISO:RGD.
DR   GO; GO:0055091; P:phospholipid homeostasis; ISO:RGD.
DR   GO; GO:0007009; P:plasma membrane organization; ISO:RGD.
DR   GO; GO:0097035; P:regulation of membrane lipid distribution; ISO:RGD.
DR   InterPro; IPR006634; TLC-dom.
DR   Pfam; PF03798; TRAM_LAG1_CLN8; 1.
DR   SMART; SM00724; TLC; 1.
DR   PROSITE; PS50922; TLC; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..247
FT                   /note="TLC domain-containing protein 1"
FT                   /id="PRO_0000285679"
FT   TOPO_DOM        28..46
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        68..83
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        105..123
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT   INTRAMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        145..173
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        195..201
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        223..247
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000250|UniProtKB:F1NZP5"
FT   DOMAIN          40..234
FT                   /note="TLC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00205"
SQ   SEQUENCE   247 AA;  28729 MW;  7DA249CC47DF82E4 CRC64;
     MPLLLHPAWP LLLGATLTFR ALRRVLCRLP LPAHVQTDPL RTWRWHNLLV SFTHSIVSGI
     WALLCIWQTP EMLVEIETAW SVCGYLLVCF SAGYFIHDTV DIVVSRQTRA SWEYLVHHVM
     AMGAFFSGIF WKRFVGGGVL TLLVEVSNIF LTLRMMMKIN NAQDILLYKV NKYVNLVMYF
     LFRLAPQAYL TKFFLQYAGQ RTLGTFLLSI LLMLDVMILI YFSRLLRSDF CPERAPSRQQ
     KDKFLTE