TLCD2_MOUSE
ID TLCD2_MOUSE Reviewed; 310 AA.
AC Q8VC26; Q9D837;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=TLC domain-containing protein 2;
GN Name=Tlcd2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Regulates the composition and fluidity of the plasma membrane
CC (By similarity). Inhibits the incorporation of membrane-fluidizing
CC phospholipids containing omega-3 long-chain polyunsaturated fatty acids
CC (LCPUFA) and thereby promotes membrane rigidity (By similarity). Does
CC not appear to have any effect on LCPUFA synthesis (By similarity).
CC {ECO:0000250|UniProtKB:A6NGC4}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:A6NGC4};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VC26-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VC26-2; Sequence=VSP_032879, VSP_032880;
CC -!- SIMILARITY: Belongs to the TLCD family. {ECO:0000305}.
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DR EMBL; AK008522; BAB25718.1; -; mRNA.
DR EMBL; AL591496; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC021945; AAH21945.1; -; mRNA.
DR CCDS; CCDS48850.1; -. [Q8VC26-1]
DR CCDS; CCDS70238.1; -. [Q8VC26-2]
DR RefSeq; NP_001278085.1; NM_001291156.1. [Q8VC26-2]
DR RefSeq; NP_081525.1; NM_027249.3. [Q8VC26-1]
DR AlphaFoldDB; Q8VC26; -.
DR STRING; 10090.ENSMUSP00000042958; -.
DR iPTMnet; Q8VC26; -.
DR PhosphoSitePlus; Q8VC26; -.
DR jPOST; Q8VC26; -.
DR MaxQB; Q8VC26; -.
DR PaxDb; Q8VC26; -.
DR PRIDE; Q8VC26; -.
DR ProteomicsDB; 259401; -. [Q8VC26-1]
DR ProteomicsDB; 259402; -. [Q8VC26-2]
DR Antibodypedia; 62302; 58 antibodies from 12 providers.
DR Ensembl; ENSMUST00000043598; ENSMUSP00000042958; ENSMUSG00000038217. [Q8VC26-1]
DR Ensembl; ENSMUST00000108435; ENSMUSP00000104074; ENSMUSG00000038217. [Q8VC26-2]
DR GeneID; 380712; -.
DR KEGG; mmu:380712; -.
DR UCSC; uc007kdw.2; mouse. [Q8VC26-1]
DR UCSC; uc011xzl.2; mouse. [Q8VC26-2]
DR CTD; 727910; -.
DR MGI; MGI:1917141; Tlcd2.
DR VEuPathDB; HostDB:ENSMUSG00000038217; -.
DR eggNOG; KOG4474; Eukaryota.
DR GeneTree; ENSGT01010000222313; -.
DR HOGENOM; CLU_056440_2_1_1; -.
DR InParanoid; Q8VC26; -.
DR OMA; AWKWRNV; -.
DR OrthoDB; 1113854at2759; -.
DR PhylomeDB; Q8VC26; -.
DR TreeFam; TF315115; -.
DR BioGRID-ORCS; 380712; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Tlcd2; mouse.
DR PRO; PR:Q8VC26; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8VC26; protein.
DR Bgee; ENSMUSG00000038217; Expressed in left lobe of liver and 179 other tissues.
DR Genevisible; Q8VC26; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0055088; P:lipid homeostasis; IBA:GO_Central.
DR GO; GO:0071709; P:membrane assembly; ISO:MGI.
DR GO; GO:0055091; P:phospholipid homeostasis; ISO:MGI.
DR GO; GO:0007009; P:plasma membrane organization; ISO:MGI.
DR GO; GO:0097035; P:regulation of membrane lipid distribution; ISO:MGI.
DR InterPro; IPR006634; TLC-dom.
DR Pfam; PF03798; TRAM_LAG1_CLN8; 1.
DR SMART; SM00724; TLC; 1.
DR PROSITE; PS50922; TLC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..310
FT /note="TLC domain-containing protein 2"
FT /id="PRO_0000328991"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 33..227
FT /note="TLC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00205"
FT VAR_SEQ 163..188
FT /note="VSSWASLATLVLFRLLPLGWMSLWLS -> APDTCTPLLLLLLVVISWYWHL
FT QNAA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032879"
FT VAR_SEQ 189..310
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032880"
SQ SEQUENCE 310 AA; 34139 MW; 58BAB5ED538EFBD6 CRC64;
MASWGLLVAG ASFTAFRGLH WGLQLLPTPK SVRDRWMWRN IFVSLIHSLL SGVGALVGLW
QFPQMVTDPI NDHPPWARVL VAVSVGYFAA DGVDMLWNQT LAQAWDLLCH HLAVVSCLST
AVVSGHYVGF SMVSLLLELN SICLHLRKLL LLSHKAPSLA FRVSSWASLA TLVLFRLLPL
GWMSLWLSRQ HYQLSLALVL LCVAGLVTVG SISISTGIRI LTKDILQSQP YPFILMHKET
KTREPVARNT STLSLKGSRY LYSTAAAALG GHLMVLASPK RCMTPSVLGL QERRLEPGKV
AHADNASTWE
