TLE3B_DANRE
ID TLE3B_DANRE Reviewed; 771 AA.
AC O13168; Q98TH2;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2017, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Transducin-like enhancer protein 3-B {ECO:0000305};
DE AltName: Full=Protein groucho-1 {ECO:0000303|PubMed:27747413};
GN Name=tle3b {ECO:0000305};
GN Synonyms=gro1 {ECO:0000303|PubMed:27747413},
GN groucho1 {ECO:0000303|PubMed:27747413};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11401394; DOI=10.1006/dbio.2001.0185;
RA Kobayashi M., Nishikawa K., Suzuki T., Yamamoto M.;
RT "The homeobox protein Six3 interacts with the Groucho corepressor and acts
RT as a transcriptional repressor in eye and forebrain formation.";
RL Dev. Biol. 232:315-326(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3] {ECO:0000312|EMBL:AAI62571.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 237-771, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=27747413; DOI=10.1007/s004270050103;
RA Wuelbeck C., Campos-Ortega J.A.;
RT "Two zebrafish homologues of the Drosophila neurogenic gene groucho and
RT their pattern of transcription during early embryogenesis.";
RL Dev. Genes Evol. 207:156-166(1997).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-296 AND SER-299, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18307296; DOI=10.1021/pr700667w;
RA Lemeer S., Pinkse M.W.H., Mohammed S., van Breukelen B., den Hertog J.,
RA Slijper M., Heck A.J.R.;
RT "Online automated in vivo zebrafish phosphoproteomics: from large-scale
RT analysis down to a single embryo.";
RL J. Proteome Res. 7:1555-1564(2008).
CC -!- FUNCTION: Transcriptional corepressor that binds to a number of
CC transcription factors. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: At gastrulation, expression is absent within the
CC axial mesoderm. After gastrulation is complete, expressed in the
CC presomitic mesoderm, but expression in the tailbud doesn't begin until
CC the six to seven somite stage, after which it becomes abundant.
CC Expression is abundant throughout somitogenesis within the posterior
CC half of the somites, but is absent from older somites. Also expressed
CC in a dynamic manner within the neural plate.
CC {ECO:0000269|PubMed:27747413}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Expression decreases from the mid-blastula stages onwards before
CC increasing again at the start of gastrulation.
CC {ECO:0000269|PubMed:27747413}.
CC -!- SIMILARITY: Belongs to the WD repeat Groucho/TLE family. {ECO:0000305}.
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DR EMBL; AB040707; BAB40698.1; -; mRNA.
DR EMBL; AL954672; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC162571; AAI62571.1; -; mRNA.
DR EMBL; U96451; AAB57808.1; -; mRNA.
DR EMBL; Y12467; CAA73070.1; -; mRNA.
DR RefSeq; NP_571855.1; NM_131780.1.
DR AlphaFoldDB; O13168; -.
DR SMR; O13168; -.
DR STRING; 7955.ENSDARP00000109120; -.
DR iPTMnet; O13168; -.
DR PaxDb; O13168; -.
DR PRIDE; O13168; -.
DR Ensembl; ENSDART00000130553; ENSDARP00000109120; ENSDARG00000069006.
DR Ensembl; ENSDART00000175980; ENSDARP00000143498; ENSDARG00000069006.
DR GeneID; 100007463; -.
DR KEGG; dre:100007463; -.
DR CTD; 100007463; -.
DR ZFIN; ZDB-GENE-990415-85; tle3b.
DR eggNOG; KOG0639; Eukaryota.
DR GeneTree; ENSGT01030000234519; -.
DR InParanoid; O13168; -.
DR OMA; FDTQMRQ; -.
DR OrthoDB; 546143at2759; -.
DR TreeFam; TF314167; -.
DR Reactome; R-DRE-201722; Formation of the beta-catenin:TCF transactivating complex.
DR Reactome; R-DRE-3769402; Deactivation of the beta-catenin transactivating complex.
DR Reactome; R-DRE-4641265; Repression of WNT target genes.
DR Reactome; R-DRE-9018519; Estrogen-dependent gene expression.
DR PRO; PR:O13168; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 7.
DR Bgee; ENSDARG00000069006; Expressed in mature ovarian follicle and 31 other tissues.
DR ExpressionAtlas; O13168; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IBA:GO_Central.
DR GO; GO:0030099; P:myeloid cell differentiation; IMP:ZFIN.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR005617; Groucho/TLE_N.
DR InterPro; IPR009146; Groucho_enhance.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR10814; PTHR10814; 1.
DR Pfam; PF03920; TLE_N; 1.
DR Pfam; PF00400; WD40; 2.
DR PRINTS; PR01850; GROUCHOFAMLY.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..771
FT /note="Transducin-like enhancer protein 3-B"
FT /id="PRO_0000051288"
FT REPEAT 483..521
FT /note="WD 1"
FT REPEAT 529..568
FT /note="WD 2"
FT REPEAT 573..612
FT /note="WD 3"
FT REPEAT 615..654
FT /note="WD 4"
FT REPEAT 656..695
FT /note="WD 5"
FT REPEAT 697..736
FT /note="WD 6"
FT REPEAT 738..771
FT /note="WD 7"
FT REGION 1..141
FT /note="Q domain"
FT /evidence="ECO:0000250|UniProtKB:Q04724"
FT REGION 137..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 142..209
FT /note="GP domain"
FT /evidence="ECO:0000250|UniProtKB:Q04724"
FT REGION 196..360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..278
FT /note="CcN domain"
FT /evidence="ECO:0000250|UniProtKB:Q04724"
FT REGION 279..451
FT /note="SP domain"
FT /evidence="ECO:0000250|UniProtKB:Q04724"
FT MOTIF 235..238
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 151..165
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..261
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 296..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 321..345
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 296
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18307296"
FT MOD_RES 299
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18307296"
FT CONFLICT 332
FT /note="P -> H (in Ref. 4; AAB57808/CAA73070)"
FT /evidence="ECO:0000305"
FT CONFLICT 350
FT /note="H -> R (in Ref. 4; AAB57808/CAA73070)"
FT /evidence="ECO:0000305"
FT CONFLICT 398
FT /note="P -> A (in Ref. 4; AAB57808/CAA73070)"
FT /evidence="ECO:0000305"
FT CONFLICT 418
FT /note="H -> S (in Ref. 4; AAB57808/CAA73070)"
FT /evidence="ECO:0000305"
FT CONFLICT 548
FT /note="V -> A (in Ref. 4; AAB57808/CAA73070)"
FT /evidence="ECO:0000305"
FT CONFLICT 552
FT /note="A -> V (in Ref. 4; AAB57808/CAA73070)"
FT /evidence="ECO:0000305"
FT CONFLICT 630
FT /note="T -> P (in Ref. 4; AAB57808/CAA73070)"
FT /evidence="ECO:0000305"
FT CONFLICT 707
FT /note="F -> S (in Ref. 4; AAB57808/CAA73070)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 771 AA; 83789 MW; 3B9CEB203826E458 CRC64;
MYPQGRHPAP HQPGQPGFKF TVAESCDRIK DEFQFLQAQY HSLKVEYDKL ANEKTEMQRH
YVMYYEMSYG LNIEMHKQTE IAKRLNAILA QIMPFLSQEH QQQVAQAVER AKQVTMTELN
AIIGVRGLPN LPLTQQQLQA QHLSHAAHGP PVQLPPHPSG LQPPGIPPVT GSGSGLLALG
ALGSQAHLPV KDEKNHHDLE HRERESSTNN SVSPSDSLRA SEKHRGSSEY SLDPKKRRVE
EKDNMSRYDS DGDKSDDLVV DVSNEDPATP RVSPSHSPPE NGLDKARALK KDAPNSPASV
ASSGSTPSSK AKDHPHNDKS STPGLKSNTP TPRNDAPTPG TSNTPGLRPH PGKPPGMEAL
TAPALRTPLS IAPPYGTPFM MGHHPEMNGS LTSPGVYPGL HISPQMSAAA AAAYGRTHMP
GFDPHPHMRA PVLPASLTSI PGGKPAYSFH VSADGQMQPV PFPPDALIGP GIPRHARQIN
TLSHGEVVCA VTISNPTRHV YTGGKGCVKI WDISQPGSKS PVSQLDCLNR DNYIRSCKLL
PDGRTLIVGG EASTLTIWDL ASQTPRIKAE LTSSAPACYA LAISPDAKVC FSCCSDGNIA
VWDLHNQTLV RQFQGHTDGA SCIDISHDGT KLWTGGLDNT VRSWDLREGR QLQQHDFTSQ
IFSLGYCPTG EWLAVGMESS NVEVLHHTKP DKYQLHLHES CVLSLKFAYC GKWFVSTGKD
NLLNAWRTPY GASIFQSKES SSVLSCDISA DDKYIVTGSG DKKATVYEVI Y
