TLE5_MOUSE
ID TLE5_MOUSE Reviewed; 197 AA.
AC P63002; Q06195;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=TLE family member 5 {ECO:0000305};
DE AltName: Full=Amino-terminal enhancer of split;
DE Short=Amino enhancer of split;
DE AltName: Full=Grg-5;
DE AltName: Full=Groucho-related protein 5;
DE AltName: Full=Protein ESP1;
DE AltName: Full=Protein GRG;
GN Name=Tle5 {ECO:0000305};
GN Synonyms=Aes {ECO:0000312|MGI:MGI:95806}, Esp1, Grg, Grg5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=8369224; DOI=10.1016/0925-4773(93)90099-j;
RA Mallo M., Franco del Amo F., Gridley T.;
RT "Cloning and developmental expression of Grg, a mouse gene related to the
RT groucho transcript of the Drosophila Enhancer of split complex.";
RL Mech. Dev. 42:67-76(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=CD-1;
RX PubMed=8365415; DOI=10.1111/j.1432-1033.1993.tb18151.x;
RA Miyasaka H., Choudhury B.K., Hou E.W., Li S.S.-L.;
RT "Molecular cloning and expression of mouse and human cDNA encoding AES and
RT ESG proteins with strong similarity to Drosophila enhancer of split groucho
RT protein.";
RL Eur. J. Biochem. 216:343-352(1993).
RN [3]
RP OLIGOMERIZATION, AND INTERACTION WITH TCF7.
RX PubMed=9783587; DOI=10.1038/26989;
RA Roose J., Molenaar M., Peterson J., Hurenkamp J., Brantjes H., Moerer P.,
RA van de Wetering M., Destree O., Clevers H.;
RT "The Xenopus Wnt effector XTcf-3 interacts with Groucho-related
RT transcriptional repressors.";
RL Nature 395:608-612(1998).
RN [4]
RP OLIGOMERIZATION, AND INTERACTION WITH TCF7.
RX PubMed=11266540; DOI=10.1093/nar/29.7.1410;
RA Brantjes H., Roose J., van De Wetering M., Clevers H.;
RT "All Tcf HMG box transcription factors interact with Groucho-related co-
RT repressors.";
RL Nucleic Acids Res. 29:1410-1419(2001).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND INTERACTION WITH
RP SIX3 AND SIX6.
RX PubMed=12050133; DOI=10.1242/dev.129.12.2835;
RA Zhu C.C., Dyer M.A., Uchikawa M., Kondoh H., Lagutin O.V., Oliver G.;
RT "Six3-mediated auto repression and eye development requires its interaction
RT with members of the Groucho-related family of co-repressors.";
RL Development 129:2835-2849(2002).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Transcriptional corepressor. Acts as dominant repressor
CC towards other family members. Inhibits NF-kappa-B-regulated gene
CC expression. May be required for the initiation and maintenance of the
CC differentiated state. Essential for the transcriptional repressor
CC activity of SIX3 during retina and lens development.
CC {ECO:0000269|PubMed:12050133}.
CC -!- SUBUNIT: Homooligomer and heterooligomer with other family members.
CC Binds TCF7 and the NF-kappa-B subunit RELA. Interacts with PHF12 (By
CC similarity). Interacts (via Q domain) with SIX3. Interacts with SIX6.
CC {ECO:0000250, ECO:0000269|PubMed:11266540, ECO:0000269|PubMed:12050133,
CC ECO:0000269|PubMed:9783587}.
CC -!- INTERACTION:
CC P63002; Q62233: Six3; NbExp=5; IntAct=EBI-646888, EBI-2297327;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12050133}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=AES-1;
CC IsoId=P63002-1, Q06195-1;
CC Sequence=Displayed;
CC Name=2; Synonyms=AES-2;
CC IsoId=P63002-2, Q06195-2;
CC Sequence=VSP_006771;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed in developing embryos by
CC midgestation, a wide expression is conserved in adult. In mouse,
CC abundantly expressed in muscle, heart and brain.
CC {ECO:0000269|PubMed:8369224}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing eye and forebrain of
CC embryos. {ECO:0000269|PubMed:12050133}.
CC -!- DOMAIN: Lacks the C-terminal WD repeats.
CC -!- PTM: Ubiquitinated by XIAP/BIRC4. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat Groucho/TLE family. {ECO:0000305}.
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DR EMBL; L12140; AAA03023.1; -; mRNA.
DR EMBL; X73359; CAA51769.1; -; mRNA.
DR EMBL; X73361; CAA51771.1; -; mRNA.
DR CCDS; CCDS24062.1; -.
DR PIR; A56675; A56675.
DR PIR; S35680; S35680.
DR RefSeq; NP_034477.1; NM_010347.4. [P63002-1]
DR AlphaFoldDB; P63002; -.
DR SMR; P63002; -.
DR BioGRID; 200056; 10.
DR CORUM; P63002; -.
DR IntAct; P63002; 5.
DR MINT; P63002; -.
DR STRING; 10090.ENSMUSP00000002518; -.
DR iPTMnet; P63002; -.
DR PhosphoSitePlus; P63002; -.
DR EPD; P63002; -.
DR MaxQB; P63002; -.
DR PaxDb; P63002; -.
DR PeptideAtlas; P63002; -.
DR PRIDE; P63002; -.
DR ProteomicsDB; 281944; -.
DR ProteomicsDB; 281945; -. [P63002-2]
DR Antibodypedia; 10920; 399 antibodies from 36 providers.
DR DNASU; 14797; -.
DR Ensembl; ENSMUST00000002518; ENSMUSP00000002518; ENSMUSG00000054452. [P63002-1]
DR GeneID; 14797; -.
DR KEGG; mmu:14797; -.
DR UCSC; uc007gim.2; mouse.
DR CTD; 166; -.
DR MGI; MGI:95806; Tle5.
DR VEuPathDB; HostDB:ENSMUSG00000054452; -.
DR eggNOG; KOG0639; Eukaryota.
DR GeneTree; ENSGT01030000234519; -.
DR HOGENOM; CLU_092092_1_0_1; -.
DR InParanoid; P63002; -.
DR OMA; GDTHQDD; -.
DR PhylomeDB; P63002; -.
DR BioGRID-ORCS; 14797; 0 hits in 74 CRISPR screens.
DR ChiTaRS; Aes; mouse.
DR PRO; PR:P63002; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; P63002; protein.
DR Bgee; ENSMUSG00000054452; Expressed in embryonic brain and 269 other tissues.
DR ExpressionAtlas; P63002; baseline and differential.
DR Genevisible; P63002; MM.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0003714; F:transcription corepressor activity; IDA:MGI.
DR GO; GO:0031668; P:cellular response to extracellular stimulus; IDA:MGI.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; ISO:MGI.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:MGI.
DR GO; GO:0032091; P:negative regulation of protein binding; ISO:MGI.
DR GO; GO:0060761; P:negative regulation of response to cytokine stimulus; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:2000210; P:positive regulation of anoikis; ISO:MGI.
DR GO; GO:0040008; P:regulation of growth; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IGI:MGI.
DR GO; GO:0070555; P:response to interleukin-1; ISO:MGI.
DR GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR005617; Groucho/TLE_N.
DR InterPro; IPR009146; Groucho_enhance.
DR PANTHER; PTHR10814; PTHR10814; 1.
DR Pfam; PF03920; TLE_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Ubl conjugation;
KW Wnt signaling pathway.
FT CHAIN 1..197
FT /note="TLE family member 5"
FT /id="PRO_0000050835"
FT REGION 166..197
FT /note="CCN domain"
FT REGION 170..197
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 176..197
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 196
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q08117"
FT VAR_SEQ 1..10
FT /note="MMFPQSRHSG -> MGGAGRRSPSCPRRA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8365415"
FT /id="VSP_006771"
SQ SEQUENCE 197 AA; 22000 MW; 2C00233AA6921A9F CRC64;
MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL ASEKSEMQRH
YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH QQQVLGAIER AKQVTAPELN
SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ PPSLPAVSAG TGLLSLSALG SQTHLSKEDK
NGHDGDTHQE DDGEKSD
