TLL_AGGAC
ID TLL_AGGAC Reviewed; 270 AA.
AC O66256;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=dTDP-6-deoxy-L-talose 4-dehydrogenase (NAD(+));
DE EC=1.1.1.339;
DE AltName: Full=dTDP-6-deoxy-L-lyxo-4-hexulose reductase;
GN Name=tll;
OS Aggregatibacter actinomycetemcomitans (Actinobacillus
OS actinomycetemcomitans) (Haemophilus actinomycetemcomitans).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Aggregatibacter.
OX NCBI_TaxID=714;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 33384 / DSM 8324 / CCUG 13227 / NCTC 9710 / Serotype c;
RX PubMed=9805002; DOI=10.1016/s0167-4781(98)00174-2;
RA Nakano Y., Yoshida Y., Yamashita Y., Koga T.;
RT "A gene cluster for 6-deoxy-L-talan synthesis in Actinobacillus
RT actinomycetemcomitans.";
RL Biochim. Biophys. Acta 1442:409-414(1998).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND GENE NAME.
RC STRAIN=ATCC 33384 / DSM 8324 / CCUG 13227 / NCTC 9710 / Serotype c;
RX PubMed=10702238; DOI=10.1074/jbc.275.10.6806;
RA Nakano Y., Suzuki N., Yoshida Y., Nezu T., Yamashita Y., Koga T.;
RT "Thymidine diphosphate-6-deoxy-L-lyxo-4-hexulose reductase synthesizing
RT dTDP-6-deoxy-L-talose from Actinobacillus actinomycetemcomitans.";
RL J. Biol. Chem. 275:6806-6812(2000).
CC -!- FUNCTION: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to
CC dTDP-6-deoxy-L-talose. {ECO:0000269|PubMed:10702238}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-6-deoxy-beta-L-talose + NAD(+) = dTDP-4-dehydro-beta-L-
CC rhamnose + H(+) + NADH; Xref=Rhea:RHEA:34447, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62830,
CC ChEBI:CHEBI:68576; EC=1.1.1.339;
CC Evidence={ECO:0000269|PubMed:10702238};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS O-antigen
CC biosynthesis. {ECO:0000269|PubMed:10702238}.
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. {ECO:0000305}.
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DR EMBL; AB010415; BAA28138.1; -; Genomic_DNA.
DR PIR; T00109; T00109.
DR RefSeq; WP_005574044.1; NZ_VSEW01000001.1.
DR AlphaFoldDB; O66256; -.
DR SMR; O66256; -.
DR KEGG; ag:BAA28138; -.
DR OMA; WIGKYVV; -.
DR UniPathway; UPA00281; -.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0009243; P:O antigen biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR001509; Epimerase_deHydtase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01370; Epimerase; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Lipopolysaccharide biosynthesis; NAD; Oxidoreductase.
FT CHAIN 1..270
FT /note="dTDP-6-deoxy-L-talose 4-dehydrogenase (NAD(+))"
FT /id="PRO_0000424092"
FT ACT_SITE 135
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 11..12
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 50..51
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 72..76
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 87
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 112
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 112
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 135
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 135
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 139
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 270 AA; 30680 MW; 33376885638DFB0F CRC64;
MNIIITGANG YIGRYVVKEL LNKGHKVIAI LFDGESPHSF LSGAELFYGD IFALSQEKKV
DLVQNAECLL HLAWQAGFNH RDPSHLNNVM KHYQFLTSMA ELGIKNISVA GTMHEVGYFV
GPIDANTPCN PRNPYGIAKN FLRQAMFDFA SVTPELNLRW LRFYYITGDD RFNNSIFTKI
LKAEDEVQEY FPLNSGEMLY DFVDIKDLSL QIEERIISKE SGIFNCCSGK PKSLRTAVEE
FIAEHNLKIK PKYNVFPARS YDSMAVWGAK