TLOV1_ARATH
ID TLOV1_ARATH Reviewed; 399 AA.
AC O64511; Q3EC83; Q8W419; Q93VM4;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Protein TWIN LOV 1;
GN Name=TLP1; Synonyms=FBX4, PLP; OrderedLocusNames=At2g02710;
GN ORFNames=T20F6.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Kiyosue T.;
RT "TLP1.";
RL Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11077244; DOI=10.1016/s1360-1385(00)01769-6;
RA Xiao W., Jang J.-C.;
RT "F-box proteins in Arabidopsis.";
RL Trends Plant Sci. 5:454-457(2000).
RN [6]
RP INTERACTION WITH VTC2; VTC5 AND BLH10.
RX PubMed=17982713; DOI=10.1007/s10265-007-0118-8;
RA Ogura Y., Komatsu A., Zikihara K., Nanjo T., Tokutomi S., Wada M.,
RA Kiyosue T.;
RT "Blue light diminishes interaction of PAS/LOV proteins, putative blue light
RT receptors in Arabidopsis thaliana, with their interacting partners.";
RL J. Plant Res. 121:97-105(2008).
CC -!- SUBUNIT: Interacts with VTC2, VTC5 and BLH10.
CC {ECO:0000269|PubMed:17982713}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=PLPB;
CC IsoId=O64511-1; Sequence=Displayed;
CC Name=2; Synonyms=PLPA;
CC IsoId=O64511-2; Sequence=VSP_022378;
CC Name=3; Synonyms=PLPC;
CC IsoId=O64511-3; Sequence=VSP_022378, VSP_022379, VSP_022380;
CC -!- PTM: FMN binds covalently to cysteine after exposure to blue light and
CC is reversed in the dark. {ECO:0000250}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to competing acceptor splice
CC site. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to contain a F-box domain.
CC {ECO:0000305|PubMed:11077244}.
CC -!- CAUTION: Contains Gly-74 instead of the conserved Cys required for the
CC covalent attachment of FMN in the first PAS domain. {ECO:0000305}.
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DR EMBL; AB038798; BAB83170.1; -; mRNA.
DR EMBL; AC002521; AAC05351.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC05613.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05614.1; -; Genomic_DNA.
DR EMBL; AY045642; AAK74000.1; -; mRNA.
DR EMBL; AY058232; AAL15406.1; -; mRNA.
DR PIR; T00857; T00857.
DR RefSeq; NP_565288.1; NM_126326.5. [O64511-1]
DR RefSeq; NP_849928.1; NM_179597.1. [O64511-2]
DR AlphaFoldDB; O64511; -.
DR SMR; O64511; -.
DR BioGRID; 202; 2.
DR IntAct; O64511; 3.
DR STRING; 3702.AT2G02710.1; -.
DR PaxDb; O64511; -.
DR PRIDE; O64511; -.
DR ProteomicsDB; 232460; -. [O64511-1]
DR EnsemblPlants; AT2G02710.1; AT2G02710.1; AT2G02710. [O64511-1]
DR EnsemblPlants; AT2G02710.2; AT2G02710.2; AT2G02710. [O64511-2]
DR GeneID; 814800; -.
DR Gramene; AT2G02710.1; AT2G02710.1; AT2G02710. [O64511-1]
DR Gramene; AT2G02710.2; AT2G02710.2; AT2G02710. [O64511-2]
DR KEGG; ath:AT2G02710; -.
DR Araport; AT2G02710; -.
DR TAIR; locus:2058852; AT2G02710.
DR eggNOG; ENOG502QPWT; Eukaryota.
DR InParanoid; O64511; -.
DR OMA; HIWVHLD; -.
DR PhylomeDB; O64511; -.
DR PRO; PR:O64511; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64511; baseline and differential.
DR Genevisible; O64511; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009881; F:photoreceptor activity; IEA:UniProtKB-KW.
DR GO; GO:0009637; P:response to blue light; IEA:UniProt.
DR CDD; cd00130; PAS; 2.
DR InterPro; IPR001610; PAC.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR000700; PAS-assoc_C.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR Pfam; PF13426; PAS_9; 2.
DR SMART; SM00086; PAC; 2.
DR SMART; SM00091; PAS; 2.
DR SUPFAM; SSF55785; SSF55785; 2.
DR TIGRFAMs; TIGR00229; sensory_box; 2.
DR PROSITE; PS50113; PAC; 2.
DR PROSITE; PS50112; PAS; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromophore; Flavoprotein; FMN;
KW Photoreceptor protein; Receptor; Reference proteome; Repeat;
KW Sensory transduction.
FT CHAIN 1..399
FT /note="Protein TWIN LOV 1"
FT /id="PRO_0000272267"
FT DOMAIN 26..97
FT /note="PAS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 98..153
FT /note="PAC 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00141"
FT DOMAIN 249..320
FT /note="PAS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT DOMAIN 320..376
FT /note="PAC 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00141"
FT MOD_RES 296
FT /note="S-4a-FMN cysteine"
FT /evidence="ECO:0000250"
FT VAR_SEQ 330..331
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_022378"
FT VAR_SEQ 354..360
FT /note="TAYFVGV -> HILWVFK (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_022379"
FT VAR_SEQ 361..399
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_022380"
SQ SEQUENCE 399 AA; 44890 MW; 9C735BE087D8A810 CRC64;
MSLTKSSESV FTEEEEEDSF SGRYTLWIKE ALEELPHNFT ITDPFISGHP IVFASLGFLK
MTGYSREEVI GRNGKVFQGP KTNRRSIMEI REAIREERSV QVSLLNYRKS GSPFWMLFHM
CPVFGKDDGK VTNFVAVQVP ISGREHHRKK LRNVGDLSSD TSPTFGSCRR EVCFGNFVCQ
DRALPVECDD DEQGLEDWEQ CEASESEKLK ATEAINNVLS ILVHYSELSG RLVCGKRYCL
RGVDCLSSSL VISLGRIKQS FVLTNPCLPD MPIIYASDAF LTLTGYKRQE VLGQNCRFLS
GVDTDSSVLY EMKECILKGQ SCTVQILNYS NRKDKSSFWN LLHISPVRNA SGKTAYFVGV
QVEASCRNTE IKELRPETRQ LSVVGAVRVA VRSSLMVTC
