TLP3_ARATH
ID TLP3_ARATH Reviewed; 406 AA.
AC Q8VY21; O82257;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Tubby-like F-box protein 3;
DE Short=AtTLP3;
GN Name=TULP3; OrderedLocusNames=At2g47900; ORFNames=F17A22.29;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=15064372; DOI=10.1104/pp.103.037820;
RA Lai C.-P., Lee C.-L., Chen P.-H., Wu S.-H., Yang C.-C., Shaw J.-F.;
RT "Molecular analyses of the Arabidopsis TUBBY-like protein gene family.";
RL Plant Physiol. 134:1586-1597(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, 3D-STRUCTURE MODELING, TISSUE SPECIFICITY,
RP INDUCTION BY H(2)O(2), SUBCELLULAR LOCATION, AND MUTAGENESIS OF LYS-187 AND
RP ARG-189.
RC STRAIN=cv. Columbia;
RX PubMed=22751378; DOI=10.1104/pp.112.201319;
RA Reitz M.U., Bissue J.K., Zocher K., Attard A., Huckelhoven R., Becker K.,
RA Imani J., Eichmann R., Schafer P.;
RT "The subcellular localization of Tubby-like proteins and participation in
RT stress signaling and root colonization by the mutualist Piriformospora
RT indica.";
RL Plant Physiol. 160:349-364(2012).
CC -!- FUNCTION: Involved in abiotic stress signaling. Tethered to plasma
CC membrane (PM) and probably bound to phosphatidylinositol 4,5-
CC bisphosphate. Abiotic stresses (drought, salt, H(2)O(2)) trigger
CC phospholipase C mediated PM dislogement and plastidial and
CC nucleocytosolic relocation of TULP3. {ECO:0000269|PubMed:22751378}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22751378};
CC Peripheral membrane protein {ECO:0000269|PubMed:22751378}. Plastid
CC {ECO:0000269|PubMed:22751378}. Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:22751378}. Cytoplasm {ECO:0000269|PubMed:22751378}.
CC Note=Phospholipase C activity mediates the release from the plasma
CC membrane and the relocation to plastids, cytoplasm and nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8VY21-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Ubiquitous at low levels. Not detected in mature
CC siliques. {ECO:0000269|PubMed:15064372, ECO:0000269|PubMed:22751378}.
CC -!- INDUCTION: Down-regulated by H(2)O(2) treatment.
CC {ECO:0000269|PubMed:22751378}.
CC -!- DISRUPTION PHENOTYPE: Reduced colonization of the roots by the
CC mutualistic fungus Piriformospora indica.
CC {ECO:0000269|PubMed:22751378}.
CC -!- SIMILARITY: Belongs to the TUB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC63644.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM15124.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY045774; AAK98802.1; -; mRNA.
DR EMBL; AC005309; AAC63644.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC006072; AAM15124.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10907.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10908.1; -; Genomic_DNA.
DR EMBL; AY074273; AAL66970.1; -; mRNA.
DR EMBL; AY096604; AAM20254.1; -; mRNA.
DR PIR; H84920; H84920.
DR RefSeq; NP_001031558.1; NM_001036481.1. [Q8VY21-1]
DR RefSeq; NP_001189775.1; NM_001202846.1.
DR RefSeq; NP_850481.1; NM_180150.2. [Q8VY21-1]
DR AlphaFoldDB; Q8VY21; -.
DR SMR; Q8VY21; -.
DR BioGRID; 4737; 6.
DR IntAct; Q8VY21; 2.
DR STRING; 3702.AT2G47900.3; -.
DR iPTMnet; Q8VY21; -.
DR PaxDb; Q8VY21; -.
DR PRIDE; Q8VY21; -.
DR EnsemblPlants; AT2G47900.1; AT2G47900.1; AT2G47900. [Q8VY21-1]
DR EnsemblPlants; AT2G47900.2; AT2G47900.2; AT2G47900. [Q8VY21-1]
DR GeneID; 819402; -.
DR Gramene; AT2G47900.1; AT2G47900.1; AT2G47900. [Q8VY21-1]
DR Gramene; AT2G47900.2; AT2G47900.2; AT2G47900. [Q8VY21-1]
DR KEGG; ath:AT2G47900; -.
DR Araport; AT2G47900; -.
DR eggNOG; KOG2502; Eukaryota.
DR HOGENOM; CLU_028236_3_0_1; -.
DR InParanoid; Q8VY21; -.
DR OrthoDB; 1445357at2759; -.
DR PhylomeDB; Q8VY21; -.
DR PRO; PR:Q8VY21; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8VY21; baseline and differential.
DR Genevisible; Q8VY21; AT.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProt.
DR Gene3D; 3.20.90.10; -; 1.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR025659; Tubby-like_C.
DR InterPro; IPR000007; Tubby_C.
DR Pfam; PF00646; F-box; 1.
DR Pfam; PF01167; Tub; 1.
DR PRINTS; PR01573; SUPERTUBBY.
DR SUPFAM; SSF54518; SSF54518; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasm; Membrane; Nucleus; Plastid;
KW Reference proteome; Stress response.
FT CHAIN 1..406
FT /note="Tubby-like F-box protein 3"
FT /id="PRO_0000272231"
FT DOMAIN 50..105
FT /note="F-box"
FT MUTAGEN 187
FT /note="K->A: Loss of plasma membrane tethering; when
FT associated with A-189."
FT /evidence="ECO:0000269|PubMed:22751378"
FT MUTAGEN 189
FT /note="R->A: Loss of plasma membrane tethering; when
FT associated with A-187."
FT /evidence="ECO:0000269|PubMed:22751378"
SQ SEQUENCE 406 AA; 45312 MW; C8A8188C0A0D77D4 CRC64;
MSFKSLIQDM RGELGSISRK GFDVRFGYGR SRSQRVVQDT SVPVDAFKQS CWASMPPELL
RDVLMRIEQS EDTWPSRKNV VSCAGVCRNW REIVKEIVRV PELSSKLTFP ISLKQPGPRG
SLVQCYIMRN RSNQTYYLYL GLNQAASNDD GKFLLAAKRF RRPTCTDYII SLNCDDVSRG
SNTYIGKLRS NFLGTKFTVY DAQPTNPGTQ VTRTRSSRLL SLKQVSPRIP SGNYPVAHIS
YELNVLGSRG PRRMQCVMDA IPASAVEPGG TAPTQTELVH SNLDSFPSFS FFRSKSIRAE
SLPSGPSSAA QKEGLLVLKN KAPRWHEQLQ CWCLNFNGRV TVASVKNFQL VAAPENGPAG
PEHENVILQF GKVGKDVFTM DYQYPISAFQ AFTICLSSFD TKIACE
