TLP_ACTCC
ID TLP_ACTCC Reviewed; 225 AA.
AC P83958; A0A2R6PIP5; L7TRX2;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2019, sequence version 2.
DT 25-MAY-2022, entry version 51.
DE RecName: Full=Thaumatin-like protein;
DE AltName: Allergen=Act c 2;
DE Flags: Precursor;
GN Name=TLP {ECO:0000305};
GN ORFNames=CEY00_Acc28854 {ECO:0000312|EMBL:PSR91510.1};
OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=1590841;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=23289429; DOI=10.1021/jf304289f;
RA Maddumage R., Nieuwenhuizen N.J., Bulley S.M., Cooney J.M., Green S.A.,
RA Atkinson R.G.;
RT "Diversity and relative levels of actinidin, kiwellin, and thaumatin-like
RT allergens in 15 varieties of kiwifruit (Actinidia).";
RL J. Agric. Food Chem. 61:728-739(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5;
RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3;
RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z.,
RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J.,
RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J.,
RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M.,
RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M.,
RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D.,
RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H.,
RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A.,
RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D.,
RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J.,
RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C.,
RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N.,
RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V.,
RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S.,
RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S.,
RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.;
RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome
RT highlights the challenges associated with draft genomes and gene prediction
RT in plants.";
RL BMC Genomics 19:257-257(2018).
RN [3]
RP PROTEIN SEQUENCE OF 25-44, AND FUNCTION.
RX PubMed=12165295; DOI=10.1016/s0031-9422(02)00144-9;
RA Wang H., Ng T.B.;
RT "Isolation of an antifungal thaumatin-like protein from kiwi fruits.";
RL Phytochemistry 61:1-6(2002).
RN [4]
RP PROTEIN SEQUENCE OF 25-37, FUNCTION, AND ALLERGEN.
RX PubMed=12417892; DOI=10.1067/mai.2002.128947;
RA Gavrovic-Jankulovic M., Cirkovic T., Vuckovic O., Atanaskovic-Markovic M.,
RA Petersen A., Gojgic G., Burazer L., Jankov R.M.;
RT "Isolation and biochemical characterization of a thaumatin-like kiwi
RT allergen.";
RL J. Allergy Clin. Immunol. 110:805-810(2002).
RN [5]
RP PROTEIN SEQUENCE OF 25-34, AND ALLERGEN.
RC STRAIN=cv. Hort 16A; TISSUE=Fruit;
RX PubMed=15536427; DOI=10.1016/j.jaci.2004.07.016;
RA Bublin M., Mari A., Ebner C., Knulst A., Scheiner O.,
RA Hoffmann-Sommergruber K., Breiteneder H., Radauer C.;
RT "IgE sensitization profiles toward green and gold kiwifruits differ among
RT patients allergic to kiwifruit from 3 European countries.";
RL J. Allergy Clin. Immunol. 114:1169-1175(2004).
CC -!- FUNCTION: Has antifungal activity against B.cinerea, C.comatus,
CC M.arachidicola, P.piricola, C.albicans and S.carlsbergensis. Inhibits
CC HIV-1 reverse transcriptase. {ECO:0000269|PubMed:12165295,
CC ECO:0000269|PubMed:12417892}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- PTM: N-glycosylated. {ECO:0000250}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE from the
CC serum of kiwi-allergic patients. {ECO:0000269|PubMed:12417892,
CC ECO:0000269|PubMed:15536427}.
CC -!- SIMILARITY: Belongs to the thaumatin family. {ECO:0000305}.
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DR EMBL; JX905283; AGC39176.1; -; mRNA.
DR EMBL; NKQK01000025; PSR91510.1; -; Genomic_DNA.
DR AlphaFoldDB; P83958; -.
DR SMR; P83958; -.
DR Allergome; 2407; Act c 2.
DR Allergome; 747; Act d 2.
DR EnsemblPlants; PSR91510; PSR91510; CEY00_Acc28854.
DR Gramene; PSR91510; PSR91510; CEY00_Acc28854.
DR OMA; KTDNYCC; -.
DR Proteomes; UP000241394; Chromosome lg25.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 2.60.110.10; -; 1.
DR InterPro; IPR037176; Osmotin/thaumatin-like_sf.
DR InterPro; IPR001938; Thaumatin.
DR InterPro; IPR017949; Thaumatin_CS.
DR PANTHER; PTHR31048; PTHR31048; 1.
DR Pfam; PF00314; Thaumatin; 1.
DR PIRSF; PIRSF002703; Thaumatin; 1.
DR PRINTS; PR00347; THAUMATIN.
DR SMART; SM00205; THN; 1.
DR SUPFAM; SSF49870; SSF49870; 1.
DR PROSITE; PS00316; THAUMATIN_1; 1.
DR PROSITE; PS51367; THAUMATIN_2; 1.
PE 1: Evidence at protein level;
KW Allergen; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW Fungicide; Glycoprotein; Pathogenesis-related protein; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000269|PubMed:12165295,
FT ECO:0000269|PubMed:12417892, ECO:0000269|PubMed:15536427"
FT CHAIN 25..225
FT /note="Thaumatin-like protein"
FT /id="PRO_0000096222"
FT DISULFID 33..224
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 74..84
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 89..95
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 140..213
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 146..196
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 154..164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 168..177
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 178..183
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT CONFLICT 29
FT /note="I -> F (in Ref. 3; AA sequence)"
SQ SEQUENCE 225 AA; 24267 MW; 987B33E3D112B64C CRC64;
MSTFKSLSLS ALLFIAFLFT CARGATFNII NNCPFTVWAA AVPGGGKRLD RGQNWIINPG
AGTKGARVWA RTGCNFDGAG RGKCQTGDCN GLLQCQAFGQ PPNTLAEYAL NQFNNLDFFD
ISLVDGFNVA MEFSPTSGGC TRGIKCTANI NEQCPNELRA PGGCNNPCTV FKTDQYCCNS
GNCGPTDYSR FFKTRCPDAY SYPKDDQTST FTCPGGTNYK VVFCP
