TLP_ACTDE
ID TLP_ACTDE Reviewed; 225 AA.
AC P81370; A5JGY0; Q5ND92;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 2.
DT 25-MAY-2022, entry version 72.
DE RecName: Full=Thaumatin-like protein;
DE AltName: Allergen=Act d 2;
DE Flags: Precursor;
GN Name=tlp;
OS Actinidia deliciosa (Kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=3627;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Fruit;
RA Radauer C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-225.
RA Gavrovic-Jankulovic M.;
RT "Thaumatin-like kiwi protein, Act d 2 allergen.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 25-53.
RC STRAIN=cv. Hayward; TISSUE=Stem plug;
RX AGRICOLA=IND22033965;
RA Wurms K.V., Greenwood D.R., Sharrock K.R., Long P.G.;
RT "Thaumatin-like protein in kiwifruit.";
RL J. Sci. Food Agric. 79:1448-1452(1999).
RN [4]
RP PROTEIN SEQUENCE OF 25-34, AND ALLERGEN.
RC STRAIN=cv. Hayward; TISSUE=Fruit;
RX PubMed=15536427; DOI=10.1016/j.jaci.2004.07.016;
RA Bublin M., Mari A., Ebner C., Knulst A., Scheiner O.,
RA Hoffmann-Sommergruber K., Breiteneder H., Radauer C.;
RT "IgE sensitization profiles toward green and gold kiwifruits differ among
RT patients allergic to kiwifruit from 3 European countries.";
RL J. Allergy Clin. Immunol. 114:1169-1175(2004).
RN [5]
RP PROTEIN SEQUENCE OF 25-32.
RC STRAIN=cv. Hayward; TISSUE=Fruit;
RX PubMed=18442249; DOI=10.1021/jf703620m;
RA Tuppo L., Giangrieco I., Palazzo P., Bernardi M.L., Scala E., Carratore V.,
RA Tamburrini M., Mari A., Ciardiello M.A.;
RT "Kiwellin, a modular protein from green and gold kiwi fruits: evidence of
RT in vivo and in vitro processing and IgE binding.";
RL J. Agric. Food Chem. 56:3812-3817(2008).
RN [6]
RP PROTEIN SEQUENCE OF 25-29, GLYCOSYLATION, AND ALLERGEN.
RC STRAIN=cv. Hayward; TISSUE=Fruit;
RX PubMed=18205857; DOI=10.1111/j.1365-2222.2007.02927.x;
RA Palacin A., Rodriguez J., Blanco C., Lopez-Torrejon G., Sanchez-Monge R.,
RA Varela J., Jimenez M.A., Cumplido J., Carrillo T., Crespo J.F., Salcedo G.;
RT "Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia
RT deliciosa) allergens in patients sensitized to Kiwi with different clinical
RT symptoms.";
RL Clin. Exp. Allergy 38:1220-1228(2008).
CC -!- FUNCTION: Has antifungal activity. {ECO:0000250}.
CC -!- INTERACTION:
CC P81370; P79085: ALTA1; Xeno; NbExp=3; IntAct=EBI-9212168, EBI-9212161;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Woody stem plug.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:18205857}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds IgE.
CC {ECO:0000269|PubMed:15536427, ECO:0000269|PubMed:18205857}.
CC -!- SIMILARITY: Belongs to the thaumatin family. {ECO:0000255|PROSITE-
CC ProRule:PRU00699}.
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DR EMBL; AJ871175; CAI38795.2; -; mRNA.
DR EMBL; EF417825; ABQ42566.1; -; Genomic_DNA.
DR PDB; 4BCT; X-ray; 0.98 A; A=25-225.
DR PDBsum; 4BCT; -.
DR AlphaFoldDB; P81370; -.
DR SMR; P81370; -.
DR IntAct; P81370; 1.
DR MINT; P81370; -.
DR Allergome; 3053; Act d 2.0101.
DR Allergome; 747; Act d 2.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 2.60.110.10; -; 1.
DR InterPro; IPR037176; Osmotin/thaumatin-like_sf.
DR InterPro; IPR001938; Thaumatin.
DR InterPro; IPR017949; Thaumatin_CS.
DR PANTHER; PTHR31048; PTHR31048; 1.
DR Pfam; PF00314; Thaumatin; 1.
DR PIRSF; PIRSF002703; Thaumatin; 1.
DR PRINTS; PR00347; THAUMATIN.
DR SMART; SM00205; THN; 1.
DR SUPFAM; SSF49870; SSF49870; 1.
DR PROSITE; PS00316; THAUMATIN_1; 1.
DR PROSITE; PS51367; THAUMATIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Antimicrobial; Direct protein sequencing;
KW Disulfide bond; Fungicide; Glycoprotein; Pathogenesis-related protein;
KW Plant defense; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000269|PubMed:15536427,
FT ECO:0000269|PubMed:18205857, ECO:0000269|PubMed:18442249,
FT ECO:0000269|Ref.3"
FT CHAIN 25..225
FT /note="Thaumatin-like protein"
FT /id="PRO_0000096223"
FT CARBOHYD 187
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 33..224
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 74..84
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 89..95
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 140..213
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 146..196
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 154..164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 168..177
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT DISULFID 178..183
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00699"
FT CONFLICT 60
FT /note="G -> S (in Ref. 2; ABQ42566)"
FT /evidence="ECO:0000305"
FT CONFLICT 176
FT /note="Y -> F (in Ref. 2; ABQ42566)"
FT /evidence="ECO:0000305"
FT STRAND 27..31
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 37..42
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 45..49
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 54..57
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 65..76
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 80..87
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 92..94
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 105..113
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 116..122
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 131..135
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 144..146
FT /evidence="ECO:0007829|PDB:4BCT"
FT HELIX 150..153
FT /evidence="ECO:0007829|PDB:4BCT"
FT HELIX 156..158
FT /evidence="ECO:0007829|PDB:4BCT"
FT HELIX 167..171
FT /evidence="ECO:0007829|PDB:4BCT"
FT HELIX 174..177
FT /evidence="ECO:0007829|PDB:4BCT"
FT HELIX 187..195
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 199..202
FT /evidence="ECO:0007829|PDB:4BCT"
FT HELIX 206..208
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 211..214
FT /evidence="ECO:0007829|PDB:4BCT"
FT STRAND 219..223
FT /evidence="ECO:0007829|PDB:4BCT"
SQ SEQUENCE 225 AA; 24221 MW; 229DABF722E7B961 CRC64;
MSTFKSLSLS ALLFIAFLFT CARGATFNII NNCPFTVWAA AVPGGGKRLD RGQNWIINPG
AGTKGARVWP RTGCNFDGAG RGKCQTGDCN GLLQCQAFGQ PPNTLAEYAL NQFNNLDFFD
ISLVDGFNVA MEFSPTSGGC TRGIKCTADI NGQCPNELRA PGGCNNPCTV FKTDQYCCNS
GNCGLTNFSK FFKDRCPDAY SYPKDDQTST FTCPAGTNYK VVFCP
