ID   BSSC_THAAR              Reviewed;          57 AA.
AC   O87942;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Benzylsuccinate synthase gamma subunit;
DE            EC=4.1.99.11;
DE   Contains:
DE     RecName: Full=Benzylsuccinate synthase gamma subunit, N-terminally processed;
GN   Name=bssC;
OS   Thauera aromatica.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC   Thauera.
OX   NCBI_TaxID=59405;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION,
RP   CATALYTIC ACTIVITY, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   PATHWAY, SUBUNIT, AND INDUCTION.
RC   STRAIN=DSM 6984 / CIP 107765 / K172;
RX   PubMed=9632263; DOI=10.1046/j.1365-2958.1998.00826.x;
RA   Leuthner B., Leutwein C., Schulz H., Horth P., Haehnel W., Schiltz E.,
RA   Schagger H., Heider J.;
RT   "Biochemical and genetic characterization of benzylsuccinate synthase from
RT   Thauera aromatica: a new glycyl radical enzyme catalysing the first step in
RT   anaerobic toluene metabolism.";
RL   Mol. Microbiol. 28:615-628(1998).
RN   [2]
RP   INDUCTION.
RC   STRAIN=DSM 6984 / CIP 107765 / K172;
RX   PubMed=9741082; DOI=10.1111/j.1574-6968.1998.tb13180.x;
RA   Leuthner B., Heider J.;
RT   "A two-component system involved in regulation of anaerobic toluene
RT   metabolism in Thauera aromatica.";
RL   FEMS Microbiol. Lett. 166:35-41(1998).
RN   [3]
RP   INDUCTION.
RC   STRAIN=DSM 6984 / CIP 107765 / K172;
RX   PubMed=11807562; DOI=10.1007/s00203-001-0375-1;
RA   Hermuth K., Leuthner B., Heider J.;
RT   "Operon structure and expression of the genes for benzylsuccinate synthase
RT   in Thauera aromatica strain K172.";
RL   Arch. Microbiol. 177:132-138(2002).
CC   -!- FUNCTION: Catalyzes the addition of fumarate to the methyl group of
CC       toluene, leading to the formation of benzylsuccinate.
CC       {ECO:0000269|PubMed:9632263}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=fumarate + toluene = 2-benzylsuccinate; Xref=Rhea:RHEA:10416,
CC         ChEBI:CHEBI:17578, ChEBI:CHEBI:29806, ChEBI:CHEBI:57621;
CC         EC=4.1.99.11; Evidence={ECO:0000269|PubMed:9632263};
CC   -!- ACTIVITY REGULATION: Activated by the benzylsuccinate synthase
CC       activating enzyme BssD. Rapidly inactivated by oxygen.
CC       {ECO:0000269|PubMed:9632263}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.0. {ECO:0000269|PubMed:9632263};
CC   -!- PATHWAY: Xenobiotic degradation; toluene degradation.
CC       {ECO:0000269|PubMed:9632263}.
CC   -!- SUBUNIT: Heterohexamer composed of 2 alpha subunits, 2 beta subunits
CC       and 2 gamma subunits. {ECO:0000269|PubMed:9632263}.
CC   -!- INDUCTION: Induced by toluene, probably via the TdiR/TdiS two-component
CC       regulatory system. {ECO:0000269|PubMed:11807562,
CC       ECO:0000269|PubMed:9632263, ECO:0000269|PubMed:9741082}.
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DR   EMBL; AJ001848; CAA05051.1; -; Genomic_DNA.
DR   AlphaFoldDB; O87942; -.
DR   SMR; O87942; -.
DR   KEGG; ag:CAA05051; -.
DR   BioCyc; MetaCyc:MON-672; -.
DR   BRENDA; 4.1.99.11; 6271.
DR   UniPathway; UPA00273; -.
DR   GO; GO:0018805; F:benzylsuccinate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042203; P:toluene catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 6.20.90.20; -; 1.
DR   InterPro; IPR013161; BssC.
DR   InterPro; IPR038640; BssC_sf.
DR   Pfam; PF08201; BssC_TutF; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Direct protein sequencing; Lyase.
FT   CHAIN           1..57
FT                   /note="Benzylsuccinate synthase gamma subunit"
FT                   /id="PRO_0000418874"
FT   INIT_MET        1
FT                   /note="Removed; alternate"
FT   CHAIN           2..57
FT                   /note="Benzylsuccinate synthase gamma subunit, N-terminally
FT                   processed"
FT                   /id="PRO_0000418875"
SQ   SEQUENCE   57 AA;  6619 MW;  11659C8F716E03AF CRC64;
     MTTCKDCAFF FSIPEDADDF EKSKGDCVTQ KDDEKGRYWL SKPVFENDQC CGAFHKR