ID   TM10B_BOVIN             Reviewed;         316 AA.
AC   Q08DP1;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=tRNA methyltransferase 10 homolog B;
DE            EC=2.1.1.221 {ECO:0000250|UniProtKB:Q6PF06};
DE   AltName: Full=RNA (guanine-9-)-methyltransferase domain-containing protein 3;
DE   AltName: Full=tRNA (guanine(9)-N(1))-methyltransferase TRMT10B;
GN   Name=TRMT10B; Synonyms=RG9MTD3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal muscle;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent guanine N(1)-
CC       methyltransferase that catalyzes the formation of N(1)-methylguanine at
CC       position 9 (m1G9) in tRNAs. Probably not able to catalyze formation of
CC       N(1)-methyladenine at position 9 (m1A9) in tRNAs.
CC       {ECO:0000250|UniProtKB:Q6PF06}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC         methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221;
CC         Evidence={ECO:0000250|UniProtKB:Q6PF06};
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. TRM10 family. {ECO:0000255|PROSITE-ProRule:PRU01012}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC123639; AAI23640.1; -; mRNA.
DR   RefSeq; NP_001070327.1; NM_001076859.1.
DR   AlphaFoldDB; Q08DP1; -.
DR   SMR; Q08DP1; -.
DR   STRING; 9913.ENSBTAP00000049012; -.
DR   Ensembl; ENSBTAT00000053555; ENSBTAP00000049012; ENSBTAG00000018922.
DR   GeneID; 516258; -.
DR   KEGG; bta:516258; -.
DR   CTD; 158234; -.
DR   VEuPathDB; HostDB:ENSBTAG00000018922; -.
DR   VGNC; VGNC:58353; TRMT10B.
DR   GeneTree; ENSGT00530000063169; -.
DR   InParanoid; Q08DP1; -.
DR   OMA; ALQAWFP; -.
DR   OrthoDB; 1396299at2759; -.
DR   Proteomes; UP000009136; Chromosome 8.
DR   Bgee; ENSBTAG00000018922; Expressed in oocyte and 109 other tissues.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IEA:Ensembl.
DR   GO; GO:0052905; F:tRNA (guanine(9)-N(1))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009019; F:tRNA (guanine-N1-)-methyltransferase activity; IBA:GO_Central.
DR   GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:Ensembl.
DR   GO; GO:0002939; P:tRNA N1-guanine methylation; IBA:GO_Central.
DR   Gene3D; 3.40.1280.30; -; 1.
DR   InterPro; IPR028564; MT_TRM10-typ.
DR   InterPro; IPR038459; MT_TRM10-typ_sf.
DR   InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR   InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR   PANTHER; PTHR13563; PTHR13563; 1.
DR   Pfam; PF01746; tRNA_m1G_MT; 1.
DR   PROSITE; PS51675; SAM_MT_TRM10; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..316
FT                   /note="tRNA methyltransferase 10 homolog B"
FT                   /id="PRO_0000311320"
FT   DOMAIN          113..310
FT                   /note="SAM-dependent MTase TRM10-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01012"
FT   REGION          77..96
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          73..98
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   316 AA;  36652 MW;  6C948916D856E453 CRC64;
     MDWKLEGSAQ KTESRVLQEH EITLDDPGED GVSESFQLLQ IDVGCEHWEQ ETLPTGSAAW
     CSKNVQRKQR HWEKIVAAKK SKRKQEKERR KANRVENSGI YPQHSKRFLR SLIKERLLEA
     KHSGPRLCID LSMTNHMSKK ELSRLAGQIR RLYGSNKKAD RPFWIYLTGF TTDSPLYEEC
     LRMNDGFSSY LLDRTEEDCF SLFPLETLVY LTPDSDHALE NVDLNKVYIL GGLVDESIQK
     KVTFQKAQEH SVKTARLPIQ EYMVKCQNGK NYHSEILTIN QVFDILSTYF ETQNWPEALK
     KGVSSRKGYV LRNSVE