TM10B_DANRE
ID TM10B_DANRE Reviewed; 310 AA.
AC Q08BM0;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=tRNA methyltransferase 10 homolog B;
DE EC=2.1.1.221 {ECO:0000250|UniProtKB:Q6PF06};
DE AltName: Full=RNA (guanine-9-)-methyltransferase domain-containing protein 3;
DE AltName: Full=tRNA (guanine(9)-N(1))-methyltransferase TRMT10B;
GN Name=trmt10b; Synonyms=rg9mtd3; ORFNames=zgc:153341;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent guanine N(1)-
CC methyltransferase that catalyzes the formation of N(1)-methylguanine at
CC position 9 (m1G9) in tRNAs. Probably not able to catalyze formation of
CC N(1)-methyladenine at position 9 (m1A9) in tRNAs.
CC {ECO:0000250|UniProtKB:Q6PF06}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221;
CC Evidence={ECO:0000250|UniProtKB:Q6PF06};
CC -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC superfamily. TRM10 family. {ECO:0000255|PROSITE-ProRule:PRU01012}.
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DR EMBL; BC124659; AAI24660.1; -; mRNA.
DR RefSeq; NP_001070749.1; NM_001077281.1.
DR AlphaFoldDB; Q08BM0; -.
DR SMR; Q08BM0; -.
DR STRING; 7955.ENSDARP00000078938; -.
DR PaxDb; Q08BM0; -.
DR GeneID; 768135; -.
DR KEGG; dre:768135; -.
DR CTD; 158234; -.
DR ZFIN; ZDB-GENE-061013-313; trmt10b.
DR eggNOG; KOG2967; Eukaryota.
DR InParanoid; Q08BM0; -.
DR PhylomeDB; Q08BM0; -.
DR PRO; PR:Q08BM0; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0052905; F:tRNA (guanine(9)-N(1))-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009019; F:tRNA (guanine-N1-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR GO; GO:0002939; P:tRNA N1-guanine methylation; IBA:GO_Central.
DR Gene3D; 3.40.1280.30; -; 1.
DR InterPro; IPR028564; MT_TRM10-typ.
DR InterPro; IPR038459; MT_TRM10-typ_sf.
DR InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR PANTHER; PTHR13563; PTHR13563; 1.
DR Pfam; PF01746; tRNA_m1G_MT; 1.
DR PROSITE; PS51675; SAM_MT_TRM10; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..310
FT /note="tRNA methyltransferase 10 homolog B"
FT /id="PRO_0000311324"
FT DOMAIN 101..298
FT /note="SAM-dependent MTase TRM10-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01012"
FT COILED 55..94
FT /evidence="ECO:0000255"
SQ SEQUENCE 310 AA; 35580 MW; 240E9835120A770A CRC64;
MEEQLRVGGC SPDGGFTIVN EAPVADLLEF LHIDVELEHA RDDREESARS KNVMRKQRNW
ERRLEVKKSK RKEEKLRKKL NRQDKDVSDA QLSKRVIKAI TKERLEGARA AGLRLCVDLS
MTEHLTHKEI SRLAAQIRRL YGSNKKALQP FHVFLTELQE DSLLYKECVG MNDGFMHYLI
DVTEESWFHL FPSEDVIYLT PDASEALESV EEDKIYILGG LVDETIHKKI SYTKAKELGV
RTARLPIDEY MVKKENPKNF HSKILAINQV FDILLTFRDT RDWTKALMAG IPPGKGFVLA
SAASKPLEET
