ID   TM10B_RAT               Reviewed;         316 AA.
AC   Q5RJK3;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=tRNA methyltransferase 10 homolog B;
DE            EC=2.1.1.221 {ECO:0000250|UniProtKB:Q6PF06};
DE   AltName: Full=RNA (guanine-9-)-methyltransferase domain-containing protein 3;
DE   AltName: Full=tRNA (guanine(9)-N(1))-methyltransferase TRMT10B;
GN   Name=Trmt10b; Synonyms=Rg9mtd3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent guanine N(1)-
CC       methyltransferase that catalyzes the formation of N(1)-methylguanine at
CC       position 9 (m1G9) in tRNAs. Probably not able to catalyze formation of
CC       N(1)-methyladenine at position 9 (m1A9) in tRNAs.
CC       {ECO:0000250|UniProtKB:Q6PF06}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC         methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221;
CC         Evidence={ECO:0000250|UniProtKB:Q6PF06};
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. TRM10 family. {ECO:0000255|PROSITE-ProRule:PRU01012}.
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DR   EMBL; BC086603; AAH86603.1; -; mRNA.
DR   RefSeq; NP_001013108.1; NM_001013090.2.
DR   RefSeq; XP_006238104.1; XM_006238042.1.
DR   RefSeq; XP_006238105.1; XM_006238043.2.
DR   RefSeq; XP_006238106.1; XM_006238044.3.
DR   AlphaFoldDB; Q5RJK3; -.
DR   SMR; Q5RJK3; -.
DR   STRING; 10116.ENSRNOP00000016719; -.
DR   PaxDb; Q5RJK3; -.
DR   PRIDE; Q5RJK3; -.
DR   Ensembl; ENSRNOT00000016720; ENSRNOP00000016719; ENSRNOG00000012454.
DR   GeneID; 298081; -.
DR   KEGG; rno:298081; -.
DR   UCSC; RGD:1310735; rat.
DR   CTD; 158234; -.
DR   RGD; 1310735; Trmt10b.
DR   eggNOG; KOG2967; Eukaryota.
DR   GeneTree; ENSGT00530000063169; -.
DR   HOGENOM; CLU_034384_8_0_1; -.
DR   InParanoid; Q5RJK3; -.
DR   OMA; ALQAWFP; -.
DR   OrthoDB; 1396299at2759; -.
DR   PhylomeDB; Q5RJK3; -.
DR   TreeFam; TF330972; -.
DR   PRO; PR:Q5RJK3; -.
DR   Proteomes; UP000002494; Chromosome 5.
DR   Bgee; ENSRNOG00000012454; Expressed in pancreas and 19 other tissues.
DR   Genevisible; Q5RJK3; RN.
DR   GO; GO:0005829; C:cytosol; ISO:RGD.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISO:RGD.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; ISO:RGD.
DR   GO; GO:0052905; F:tRNA (guanine(9)-N(1))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009019; F:tRNA (guanine-N1-)-methyltransferase activity; ISO:RGD.
DR   GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; ISO:RGD.
DR   GO; GO:0002939; P:tRNA N1-guanine methylation; IBA:GO_Central.
DR   Gene3D; 3.40.1280.30; -; 1.
DR   InterPro; IPR028564; MT_TRM10-typ.
DR   InterPro; IPR038459; MT_TRM10-typ_sf.
DR   InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR   InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR   PANTHER; PTHR13563; PTHR13563; 1.
DR   Pfam; PF01746; tRNA_m1G_MT; 1.
DR   PROSITE; PS51675; SAM_MT_TRM10; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..316
FT                   /note="tRNA methyltransferase 10 homolog B"
FT                   /id="PRO_0000311323"
FT   DOMAIN          113..310
FT                   /note="SAM-dependent MTase TRM10-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01012"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          42..98
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          75..96
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        71..87
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   316 AA;  36430 MW;  8106B282C65C2953 CRC64;
     MDCEWEGRPQ RAGSRASQDP EGLPEARDDG LSESFLLLQM DVEYDPQEKR RPANSAAWSS
     KNVQRKQRHW ERIVSSKKSK RKQERERRKI KRAEDLGNGT CPQHSKRFLK ALTKEKLLEA
     KHSGPRLCVD LSMTQHMSKK ELSRLAGQIR RLYGSNKKAS RPFWIYLTGF STDSPLYEEC
     LRMNDGFSAY VLDVTEEDCF SLFPLETLVY LTPDSEHPLE DIDLSTVYII GGLVDESIQK
     KVTFQKAQEY SVKTARLPIQ EHMIRCQNEK NFHSEILAIN QVFDILSAYL ETRDWPEALK
     KGVSPGKGYI LQNSVE