TM143_HUMAN
ID TM143_HUMAN Reviewed; 459 AA.
AC Q96AN5; A8K656; Q6UXY4; Q9NV49;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Transmembrane protein 143;
GN Name=TMEM143; ORFNames=UNQ5922/PRO19813;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Ovarian carcinoma, and Pericardium;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Duodenum;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC Q96AN5; O95870: ABHD16A; NbExp=3; IntAct=EBI-13342951, EBI-348517;
CC Q96AN5; Q13520: AQP6; NbExp=3; IntAct=EBI-13342951, EBI-13059134;
CC Q96AN5; Q8N6G5: CSGALNACT2; NbExp=3; IntAct=EBI-13342951, EBI-10267100;
CC Q96AN5; Q6ZPD8: DGAT2L6; NbExp=3; IntAct=EBI-13342951, EBI-12831978;
CC Q96AN5; Q8N5I4: DHRSX; NbExp=3; IntAct=EBI-13342951, EBI-3923585;
CC Q96AN5; Q9H400: LIME1; NbExp=3; IntAct=EBI-13342951, EBI-2830566;
CC Q96AN5; Q8N112: LSMEM2; NbExp=3; IntAct=EBI-13342951, EBI-10264855;
CC Q96AN5; Q9UHE5: NAT8; NbExp=3; IntAct=EBI-13342951, EBI-2863634;
CC Q96AN5; Q9P0S3: ORMDL1; NbExp=3; IntAct=EBI-13342951, EBI-1054848;
CC Q96AN5; Q96HR9-2: REEP6; NbExp=3; IntAct=EBI-13342951, EBI-14065960;
CC Q96AN5; O95562: SFT2D2; NbExp=3; IntAct=EBI-13342951, EBI-4402330;
CC Q96AN5; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-13342951, EBI-18159983;
CC Q96AN5; A0PK00: TMEM120B; NbExp=3; IntAct=EBI-13342951, EBI-10171534;
CC Q96AN5; Q9BWQ6: YIPF2; NbExp=3; IntAct=EBI-13342951, EBI-751204;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q96AN5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96AN5-2; Sequence=VSP_024930;
CC Name=3;
CC IsoId=Q96AN5-3; Sequence=VSP_024931, VSP_024932;
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DR EMBL; AY358161; AAQ88528.1; -; mRNA.
DR EMBL; AK001784; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK291521; BAF84210.1; -; mRNA.
DR EMBL; CH471177; EAW52337.1; -; Genomic_DNA.
DR EMBL; BC016919; AAH16919.1; -; mRNA.
DR CCDS; CCDS12716.1; -. [Q96AN5-1]
DR CCDS; CCDS77325.1; -. [Q96AN5-2]
DR RefSeq; NP_001290469.1; NM_001303540.1. [Q96AN5-2]
DR RefSeq; NP_060743.2; NM_018273.3. [Q96AN5-1]
DR AlphaFoldDB; Q96AN5; -.
DR BioGRID; 120551; 35.
DR IntAct; Q96AN5; 20.
DR STRING; 9606.ENSP00000293261; -.
DR iPTMnet; Q96AN5; -.
DR PhosphoSitePlus; Q96AN5; -.
DR BioMuta; TMEM143; -.
DR DMDM; 74731136; -.
DR EPD; Q96AN5; -.
DR jPOST; Q96AN5; -.
DR MassIVE; Q96AN5; -.
DR MaxQB; Q96AN5; -.
DR PaxDb; Q96AN5; -.
DR PeptideAtlas; Q96AN5; -.
DR PRIDE; Q96AN5; -.
DR ProteomicsDB; 75976; -. [Q96AN5-1]
DR ProteomicsDB; 75977; -. [Q96AN5-2]
DR Antibodypedia; 3033; 64 antibodies from 16 providers.
DR DNASU; 55260; -.
DR Ensembl; ENST00000293261.8; ENSP00000293261.2; ENSG00000161558.11. [Q96AN5-1]
DR Ensembl; ENST00000377431.6; ENSP00000366649.1; ENSG00000161558.11. [Q96AN5-2]
DR GeneID; 55260; -.
DR KEGG; hsa:55260; -.
DR MANE-Select; ENST00000293261.8; ENSP00000293261.2; NM_018273.4; NP_060743.2.
DR UCSC; uc002pix.2; human. [Q96AN5-1]
DR CTD; 55260; -.
DR GeneCards; TMEM143; -.
DR HGNC; HGNC:25603; TMEM143.
DR HPA; ENSG00000161558; Tissue enhanced (skeletal muscle, tongue).
DR neXtProt; NX_Q96AN5; -.
DR OpenTargets; ENSG00000161558; -.
DR PharmGKB; PA144596251; -.
DR VEuPathDB; HostDB:ENSG00000161558; -.
DR eggNOG; ENOG502QR4I; Eukaryota.
DR GeneTree; ENSGT00940000154315; -.
DR InParanoid; Q96AN5; -.
DR OMA; ERFIPFR; -.
DR OrthoDB; 1506124at2759; -.
DR PhylomeDB; Q96AN5; -.
DR TreeFam; TF329116; -.
DR PathwayCommons; Q96AN5; -.
DR SignaLink; Q96AN5; -.
DR BioGRID-ORCS; 55260; 16 hits in 1085 CRISPR screens.
DR GenomeRNAi; 55260; -.
DR Pharos; Q96AN5; Tdark.
DR PRO; PR:Q96AN5; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q96AN5; protein.
DR Bgee; ENSG00000161558; Expressed in hindlimb stylopod muscle and 154 other tissues.
DR ExpressionAtlas; Q96AN5; baseline and differential.
DR Genevisible; Q96AN5; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IDA:LIFEdb.
DR InterPro; IPR022227; DUF3754.
DR Pfam; PF12576; DUF3754; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..459
FT /note="Transmembrane protein 143"
FT /id="PRO_0000285958"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 435..459
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 332
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8VD26"
FT VAR_SEQ 88..187
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12975309,
FT ECO:0000303|PubMed:14702039"
FT /id="VSP_024930"
FT VAR_SEQ 89..128
FT /note="EFHSSPAEKAALEAFSAHVDFCTLFHYHQILARLQALYDP -> VTGIPLES
FT GREGGFGGVLSPRGLLHPVPLPPNPGPAAGLI (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_024931"
FT VAR_SEQ 129..459
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_024932"
FT VARIANT 143
FT /note="D -> N (in dbSNP:rs34488893)"
FT /id="VAR_032058"
SQ SEQUENCE 459 AA; 51715 MW; E2BF3166AF38BF80 CRC64;
MTVELWLRLR GKGLAMLHVT RGVWGSRVRV WPLLPALLGP PRALSSLAAK MGEYRKMWNP
REPRDWAQQY RERFIPFSKE QLLRLLIQEF HSSPAEKAAL EAFSAHVDFC TLFHYHQILA
RLQALYDPIN PDRETLDQPS LTDPQRLSNE QEVLRALEPL LAQANFSPLS EDTLAYALVV
HHPQDEVQVT VNLDQYVYIH FWALGQRVGQ MPLKSSVGSR RGFFTKLPPA ERRYFKRVVL
AARTKRGHLV LKSFKDTPLE GLEQLLPELK VRTPTLQRAL LNLMLVVSGV AIFVNVGMVV
LTDLKVATSL LLLLFAIFMG LRASKMFGQR RSAQALELAH MLYYRSTSNN SELLSALALR
AQDEHTKEAL LAHSFLARRP GGTQGSPEET SRWLRSEVEN WLLAKSGCEV TFNGTRALAH
LQALTPSMGL YPPPGFPKLD PVAPITSEPP QATPSSNIS
