ID   TM147_RAT               Reviewed;         224 AA.
AC   Q2TA63;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Transmembrane protein 147;
GN   Name=Tmem147;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of a ribosome-associated endoplasmic reticulum (ER)
CC       translocon complex involved in multi-pass membrane protein transport
CC       into the ER membrane and biogenesis. Together with SEC61 and TMCO1,
CC       forms the lipid-filled cavity at the center of the translocon where
CC       TMEM147 may insert hydrophobic segments of mutli-pass membrane proteins
CC       from the lumen into de central membrane cavity in a process gated by
CC       SEC61, and TMCO1 may insert hydrophobic segments of nascent chains from
CC       the cytosol into the cavity. Acts as a negative regulator of CHRM3
CC       function, most likely by interfering with its trafficking to the cell
CC       membrane. Negatively regulates CHRM3-mediated calcium mobilization and
CC       activation of RPS6KA1/p90RSK activity. {ECO:0000250|UniProtKB:Q9BVK8}.
CC   -!- SUBUNIT: Forms a complex with NCLN/Nicalin and NOMO, resulting in a
CC       stabilization of the 3 proteins, which are otherwise quickly degraded
CC       by the proteasome. Interacts with CHRM3, CHRM1 and AVPR2. The ribosome-
CC       associated ER translocon complex includes SEC61A1, SEC61B, SEC61G,
CC       TMCO1, CCDC47, NCLN/Nicalin, NOMO and TMEM147; in the absence of
CC       ribosomes, only the complex forms with NCLN/Nicalin, NOMO and TMEM147
CC       remains intact. {ECO:0000250|UniProtKB:Q9BVK8}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q9BVK8}; Multi-pass membrane protein
CC       {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:I6VSD2}; Multi-pass
CC       membrane protein {ECO:0000255}.
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DR   EMBL; BC111082; AAI11083.1; -; mRNA.
DR   RefSeq; NP_001033583.1; NM_001038494.1.
DR   AlphaFoldDB; Q2TA63; -.
DR   SMR; Q2TA63; -.
DR   STRING; 10116.ENSRNOP00000028514; -.
DR   PaxDb; Q2TA63; -.
DR   GeneID; 292792; -.
DR   KEGG; rno:292792; -.
DR   UCSC; RGD:1304706; rat.
DR   CTD; 10430; -.
DR   RGD; 1304706; Tmem147.
DR   VEuPathDB; HostDB:ENSRNOG00000021006; -.
DR   eggNOG; KOG3236; Eukaryota.
DR   HOGENOM; CLU_086813_0_0_1; -.
DR   InParanoid; Q2TA63; -.
DR   OMA; MKSTVDL; -.
DR   OrthoDB; 1388283at2759; -.
DR   PhylomeDB; Q2TA63; -.
DR   TreeFam; TF314086; -.
DR   PRO; PR:Q2TA63; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000021006; Expressed in pancreas and 19 other tissues.
DR   Genevisible; Q2TA63; RN.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0032991; C:protein-containing complex; ISO:RGD.
DR   GO; GO:0043022; F:ribosome binding; ISS:UniProtKB.
DR   InterPro; IPR019164; TMEM147.
DR   PANTHER; PTHR12869; PTHR12869; 1.
DR   Pfam; PF09767; DUF2053; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..224
FT                   /note="Transmembrane protein 147"
FT                   /id="PRO_0000271703"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        38..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        167..187
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   224 AA;  25291 MW;  90122F32D71E53AE CRC64;
     MTLFHFGNCF ALAYFPYFIT YKCSGLSEYN AFWKCVQAGV TYLFVQLCKM LFLATFFPTW
     EGGIYDFIGE FMKASVDVAD LIGLNLVMSR NAGKGEYKIM VAALGWATAE LIMSRCIPLW
     VGARGIEFDW KYIQMSIDSN ISLVHYIVAS AQVWMITRYD LYHTFRPAVL LLMFLSVYKA
     FVMETFVHLC SLGSWTALLA RAVVTGLLAL STLALYVAVV NVHS