TM14A_HUMAN
ID TM14A_HUMAN Reviewed; 99 AA.
AC Q9Y6G1; B2R552;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Transmembrane protein 14A;
GN Name=TMEM14A; Synonyms=C6orf73; ORFNames=PTD011;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Pituitary tumor;
RA Zhou J., Huang Q., Song H., Peng J., Zhang Q., Fu G., Dai M., Mao Y.,
RA Mao M., Chen Z., Chen J.;
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Fetal brain;
RA Tong X., Liu B., Sun Z.;
RL Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=21723035; DOI=10.1016/j.canlet.2011.05.031;
RA Woo I.S., Jin H., Kang E.S., Kim H.J., Lee J.H., Chang K.C., Park J.Y.,
RA Choi W.S., Seo H.G.;
RT "TMEM14A inhibits N-(4-hydroxyphenyl)retinamide-induced apoptosis through
RT the stabilization of mitochondrial membrane potential.";
RL Cancer Lett. 309:190-198(2011).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=26896463; DOI=10.1042/bsr20150258;
RA Zhang Q., Chen X., Zhang X., Zhan J., Chen J.;
RT "Knockdown of TMEM14A expression by RNAi inhibits the proliferation and
RT invasion of human ovarian cancer cells.";
RL Biosci. Rep. 36:E00298-E00298(2016).
RN [9]
RP STRUCTURE BY NMR.
RX PubMed=22609626; DOI=10.1038/nmeth.2033;
RA Klammt C., Maslennikov I., Bayrhuber M., Eichmann C., Vajpai N., Chiu E.J.,
RA Blain K.Y., Esquivies L., Kwon J.H., Balana B., Pieper U., Sali A.,
RA Slesinger P.A., Kwiatkowski W., Riek R., Choe S.;
RT "Facile backbone structure determination of human membrane proteins by NMR
RT spectroscopy.";
RL Nat. Methods 9:834-839(2012).
CC -!- FUNCTION: Inhibits apoptosis via negative regulation of the
CC mitochondrial outer membrane permeabilization involved in apoptotic
CC signaling pathway. {ECO:0000269|PubMed:21723035}.
CC -!- INTERACTION:
CC Q9Y6G1; Q13520: AQP6; NbExp=3; IntAct=EBI-2800360, EBI-13059134;
CC Q9Y6G1; Q7Z7G2: CPLX4; NbExp=3; IntAct=EBI-2800360, EBI-18013275;
CC Q9Y6G1; Q96BA8: CREB3L1; NbExp=5; IntAct=EBI-2800360, EBI-6942903;
CC Q9Y6G1; P49682: CXCR3; NbExp=3; IntAct=EBI-2800360, EBI-12836456;
CC Q9Y6G1; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-2800360, EBI-781551;
CC Q9Y6G1; Q96PL5: ERMAP; NbExp=3; IntAct=EBI-2800360, EBI-13361852;
CC Q9Y6G1; Q9Y5U9: IER3IP1; NbExp=3; IntAct=EBI-2800360, EBI-725665;
CC Q9Y6G1; O95279: KCNK5; NbExp=3; IntAct=EBI-2800360, EBI-3934936;
CC Q9Y6G1; Q9Y2L9-3: LRCH1; NbExp=3; IntAct=EBI-2800360, EBI-12082218;
CC Q9Y6G1; Q9BS40: LXN; NbExp=3; IntAct=EBI-2800360, EBI-1044504;
CC Q9Y6G1; Q8N4V1: MMGT1; NbExp=3; IntAct=EBI-2800360, EBI-6163737;
CC Q9Y6G1; P15941-11: MUC1; NbExp=3; IntAct=EBI-2800360, EBI-17263240;
CC Q9Y6G1; O14524-2: NEMP1; NbExp=3; IntAct=EBI-2800360, EBI-10969203;
CC Q9Y6G1; Q13113: PDZK1IP1; NbExp=3; IntAct=EBI-2800360, EBI-716063;
CC Q9Y6G1; Q9NY72: SCN3B; NbExp=3; IntAct=EBI-2800360, EBI-17247926;
CC Q9Y6G1; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-2800360, EBI-18159983;
CC Q9Y6G1; P54219-3: SLC18A1; NbExp=3; IntAct=EBI-2800360, EBI-17595455;
CC Q9Y6G1; Q9BZV2: SLC19A3; NbExp=3; IntAct=EBI-2800360, EBI-3923779;
CC Q9Y6G1; P30825: SLC7A1; NbExp=3; IntAct=EBI-2800360, EBI-4289564;
CC Q9Y6G1; Q8WWF3: SSMEM1; NbExp=3; IntAct=EBI-2800360, EBI-17280858;
CC Q9Y6G1; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-2800360, EBI-8638294;
CC Q9Y6G1; Q7Z7N9: TMEM179B; NbExp=3; IntAct=EBI-2800360, EBI-11724423;
CC Q9Y6G1; Q9NW97: TMEM51; NbExp=3; IntAct=EBI-2800360, EBI-726044;
CC Q9Y6G1; Q96HE8: TMEM80; NbExp=3; IntAct=EBI-2800360, EBI-11742770;
CC Q9Y6G1; Q8N661: TMEM86B; NbExp=3; IntAct=EBI-2800360, EBI-2548832;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:21723035}; Multi-pass membrane protein
CC {ECO:0000255}. Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:21723035}.
CC -!- TISSUE SPECIFICITY: Expressed at significantly higher levels in ovarian
CC cancer tissues than in normal tissues (at protein level).
CC {ECO:0000269|PubMed:26896463}.
CC -!- SIMILARITY: Belongs to the TMEM14 family. {ECO:0000305}.
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DR EMBL; AF078864; AAD44496.1; -; mRNA.
DR EMBL; AF239771; AAF59948.1; -; mRNA.
DR EMBL; AK312063; BAG34999.1; -; mRNA.
DR EMBL; AL109918; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471081; EAX04380.1; -; Genomic_DNA.
DR EMBL; BC015097; AAH15097.1; -; mRNA.
DR EMBL; BC019328; AAH19328.1; -; mRNA.
DR CCDS; CCDS4943.1; -.
DR RefSeq; NP_054770.1; NM_014051.3.
DR PDB; 2LOO; NMR; -; A=1-99.
DR PDB; 2LOP; NMR; -; A=1-99.
DR PDBsum; 2LOO; -.
DR PDBsum; 2LOP; -.
DR AlphaFoldDB; Q9Y6G1; -.
DR BMRB; Q9Y6G1; -.
DR SMR; Q9Y6G1; -.
DR BioGRID; 118801; 37.
DR IntAct; Q9Y6G1; 34.
DR MINT; Q9Y6G1; -.
DR STRING; 9606.ENSP00000211314; -.
DR iPTMnet; Q9Y6G1; -.
DR PhosphoSitePlus; Q9Y6G1; -.
DR BioMuta; TMEM14A; -.
DR DMDM; 9910842; -.
DR MassIVE; Q9Y6G1; -.
DR PaxDb; Q9Y6G1; -.
DR PeptideAtlas; Q9Y6G1; -.
DR PRIDE; Q9Y6G1; -.
DR ProteomicsDB; 86673; -.
DR TopDownProteomics; Q9Y6G1; -.
DR Antibodypedia; 30922; 37 antibodies from 11 providers.
DR DNASU; 28978; -.
DR Ensembl; ENST00000211314.5; ENSP00000211314.4; ENSG00000096092.6.
DR GeneID; 28978; -.
DR KEGG; hsa:28978; -.
DR MANE-Select; ENST00000211314.5; ENSP00000211314.4; NM_014051.4; NP_054770.1.
DR UCSC; uc003pax.4; human.
DR CTD; 28978; -.
DR DisGeNET; 28978; -.
DR GeneCards; TMEM14A; -.
DR HGNC; HGNC:21076; TMEM14A.
DR HPA; ENSG00000096092; Low tissue specificity.
DR neXtProt; NX_Q9Y6G1; -.
DR OpenTargets; ENSG00000096092; -.
DR PharmGKB; PA134974524; -.
DR VEuPathDB; HostDB:ENSG00000096092; -.
DR eggNOG; KOG4267; Eukaryota.
DR GeneTree; ENSGT00940000158206; -.
DR HOGENOM; CLU_096652_4_3_1; -.
DR InParanoid; Q9Y6G1; -.
DR OMA; MGTRYKK; -.
DR OrthoDB; 1642026at2759; -.
DR PhylomeDB; Q9Y6G1; -.
DR TreeFam; TF323345; -.
DR PathwayCommons; Q9Y6G1; -.
DR SignaLink; Q9Y6G1; -.
DR BioGRID-ORCS; 28978; 13 hits in 1083 CRISPR screens.
DR ChiTaRS; TMEM14A; human.
DR GenomeRNAi; 28978; -.
DR Pharos; Q9Y6G1; Tbio.
DR PRO; PR:Q9Y6G1; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q9Y6G1; protein.
DR Bgee; ENSG00000096092; Expressed in oocyte and 202 other tissues.
DR Genevisible; Q9Y6G1; HS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IDA:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
DR GO; GO:1901029; P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway; IMP:UniProtKB.
DR GO; GO:0070453; P:regulation of heme biosynthetic process; IBA:GO_Central.
DR Gene3D; 1.10.10.1740; -; 1.
DR InterPro; IPR005349; TMEM14.
DR InterPro; IPR044890; TMEM14_sf.
DR PANTHER; PTHR12668; PTHR12668; 1.
DR Pfam; PF03647; Tmemb_14; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Apoptosis; Endoplasmic reticulum; Membrane; Mitochondrion;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..99
FT /note="Transmembrane protein 14A"
FT /id="PRO_0000221169"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 37
FT /note="C -> R (in dbSNP:rs11543266)"
FT /id="VAR_052497"
FT HELIX 6..19
FT /evidence="ECO:0007829|PDB:2LOO"
FT HELIX 29..46
FT /evidence="ECO:0007829|PDB:2LOO"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:2LOP"
FT HELIX 54..67
FT /evidence="ECO:0007829|PDB:2LOO"
FT STRAND 73..80
FT /evidence="ECO:0007829|PDB:2LOO"
FT HELIX 81..94
FT /evidence="ECO:0007829|PDB:2LOO"
SQ SEQUENCE 99 AA; 10712 MW; D41CBB95241969B3 CRC64;
MDLIGFGYAA LVTFGSIFGY KRRGGVPSLI AGLFVGCLAG YGAYRVSNDK RDVKVSLFTA
FFLATIMGVR FKRSKKIMPA GLVAGLSLMM ILRLVLLLL
