TM163_HUMAN
ID TM163_HUMAN Reviewed; 289 AA.
AC Q8TC26; Q53QM3; Q53SV7; Q69YH3; Q9UFG3;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Transmembrane protein 163;
GN Name=TMEM163;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Kidney, and Stomach;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May bind zinc and other divalent cations and recruit them to
CC vesicular organelles. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic
CC vesicle membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Early endosome membrane {ECO:0000250}.
CC Note=Glutamatergic synaptic vesicles. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8TC26-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TC26-2; Sequence=VSP_023329, VSP_023330, VSP_023331,
CC VSP_023332;
CC Name=3;
CC IsoId=Q8TC26-3; Sequence=VSP_023328;
CC -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 3]: Dubious isoform that could be a cloning
CC artifact. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TMEM163 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAH10592.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL122044; CAB59180.2; -; mRNA.
DR EMBL; AL833765; CAH10592.1; ALT_INIT; mRNA.
DR EMBL; AC013718; AAX93249.1; -; Genomic_DNA.
DR EMBL; AC110620; AAY24144.1; -; Genomic_DNA.
DR EMBL; BC026170; AAH26170.1; -; mRNA.
DR CCDS; CCDS2172.1; -. [Q8TC26-1]
DR PIR; T34524; T34524.
DR RefSeq; NP_112185.1; NM_030923.4. [Q8TC26-1]
DR AlphaFoldDB; Q8TC26; -.
DR SMR; Q8TC26; -.
DR BioGRID; 123550; 1.
DR IntAct; Q8TC26; 1.
DR STRING; 9606.ENSP00000281924; -.
DR TCDB; 2.A.4.8.3; the cation diffusion facilitator (cdf) family.
DR iPTMnet; Q8TC26; -.
DR PhosphoSitePlus; Q8TC26; -.
DR BioMuta; TMEM163; -.
DR DMDM; 74730546; -.
DR jPOST; Q8TC26; -.
DR MassIVE; Q8TC26; -.
DR PaxDb; Q8TC26; -.
DR PeptideAtlas; Q8TC26; -.
DR PRIDE; Q8TC26; -.
DR ProteomicsDB; 74081; -. [Q8TC26-1]
DR ProteomicsDB; 74082; -. [Q8TC26-2]
DR ProteomicsDB; 74083; -. [Q8TC26-3]
DR Antibodypedia; 33555; 40 antibodies from 14 providers.
DR DNASU; 81615; -.
DR Ensembl; ENST00000281924.6; ENSP00000281924.6; ENSG00000152128.13. [Q8TC26-1]
DR GeneID; 81615; -.
DR KEGG; hsa:81615; -.
DR MANE-Select; ENST00000281924.6; ENSP00000281924.6; NM_030923.5; NP_112185.1.
DR UCSC; uc002ttx.4; human. [Q8TC26-1]
DR CTD; 81615; -.
DR DisGeNET; 81615; -.
DR GeneCards; TMEM163; -.
DR HGNC; HGNC:25380; TMEM163.
DR HPA; ENSG00000152128; Tissue enhanced (brain, pancreas).
DR MIM; 618978; gene.
DR neXtProt; NX_Q8TC26; -.
DR OpenTargets; ENSG00000152128; -.
DR PharmGKB; PA145148025; -.
DR VEuPathDB; HostDB:ENSG00000152128; -.
DR eggNOG; ENOG502QW7B; Eukaryota.
DR GeneTree; ENSGT00390000001170; -.
DR HOGENOM; CLU_081161_0_0_1; -.
DR InParanoid; Q8TC26; -.
DR OMA; IMRYSAS; -.
DR OrthoDB; 1547703at2759; -.
DR PhylomeDB; Q8TC26; -.
DR TreeFam; TF330782; -.
DR PathwayCommons; Q8TC26; -.
DR SignaLink; Q8TC26; -.
DR BioGRID-ORCS; 81615; 13 hits in 1071 CRISPR screens.
DR ChiTaRS; TMEM163; human.
DR GenomeRNAi; 81615; -.
DR Pharos; Q8TC26; Tbio.
DR PRO; PR:Q8TC26; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8TC26; protein.
DR Bgee; ENSG00000152128; Expressed in oocyte and 149 other tissues.
DR Genevisible; Q8TC26; HS.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0031901; C:early endosome membrane; ISS:UniProtKB.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; ISS:UniProtKB.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR GO; GO:0099180; P:zinc ion import into synaptic vesicle; IEA:Ensembl.
DR Gene3D; 1.20.1510.10; -; 1.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR InterPro; IPR026765; Tmem163.
DR PANTHER; PTHR31937; PTHR31937; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasmic vesicle; Endosome; Membrane;
KW Phosphoprotein; Reference proteome; Synapse; Transmembrane;
KW Transmembrane helix; Zinc.
FT CHAIN 1..289
FT /note="Transmembrane protein 163"
FT /id="PRO_0000278539"
FT TOPO_DOM 1..88
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..116
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..150
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..187
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..217
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..255
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..289
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..40
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:A9CMA6"
FT MOD_RES 55
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8C996"
FT MOD_RES 57
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8C996"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8C996"
FT VAR_SEQ 1..208
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_023328"
FT VAR_SEQ 1..60
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_023329"
FT VAR_SEQ 61..68
FT /note="SDGLEDRG -> MLPVSRTC (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_023330"
FT VAR_SEQ 153..162
FT /note="IACVILGVIF -> MTISCSVSPF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_023331"
FT VAR_SEQ 163..289
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:17974005"
FT /id="VSP_023332"
SQ SEQUENCE 289 AA; 31469 MW; 0255DAE6D7E8487A CRC64;
MEPAAGIQRR SSQGPTVPPP PRGHAPPAAA PGPAPLSSPV REPPQLEEER QVRISESGQF
SDGLEDRGLL ESSTRLKPHE AQNYRKKALW VSWFSIIVTL ALAVAAFTVS VMRYSASAFG
FAFDAILDVL SSAIVLWRYS NAAAVHSAHR EYIACVILGV IFLLSSICIV VKAIHDLSTR
LLPEVDDFLF SVSILSGILC SILAVLKFML GKVLTSRALI TDGFNSLVGG VMGFSILLSA
EVFKHDSAVW YLDGSIGVLI GLTIFAYGVK LLIDMVPRVR QTRHYEMFE
