TM163_MOUSE
ID TM163_MOUSE Reviewed; 288 AA.
AC Q8C996; Q8C8I2; Q9D0E0;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Transmembrane protein 163;
DE AltName: Full=Synaptic vesicle membrane protein of 31 kDa;
GN Name=Tmem163; Synonyms=Sv31;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION, TOPOLOGY, AND TISSUE SPECIFICITY.
RX PubMed=17623043; DOI=10.1111/j.1471-4159.2007.04758.x;
RA Burre J., Zimmermann H., Volknandt W.;
RT "Identification and characterization of SV31, a novel synaptic vesicle
RT membrane protein and potential transporter.";
RL J. Neurochem. 103:276-287(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain cortex;
RX PubMed=17114649; DOI=10.1074/mcp.m600046-mcp200;
RA Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,
RA Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B.,
RA Panse C., Schlapbach R., Mansuy I.M.;
RT "Qualitative and quantitative analyses of protein phosphorylation in naive
RT and stimulated mouse synaptosomal preparations.";
RL Mol. Cell. Proteomics 6:283-293(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-54; SER-56 AND
RP SER-60, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May bind zinc and other divalent cations and recruit them to
CC vesicular organelles. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic
CC vesicle membrane {ECO:0000269|PubMed:17623043}; Multi-pass membrane
CC protein {ECO:0000269|PubMed:17623043}. Early endosome membrane
CC {ECO:0000250}. Note=Glutamatergic synaptic vesicles.
CC -!- TISSUE SPECIFICITY: Specifically expressed in brain. Mainly expressed
CC in the glutaminergic neuron subpopulations.
CC {ECO:0000269|PubMed:17623043}.
CC -!- SIMILARITY: Belongs to the TMEM163 family. {ECO:0000305}.
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DR EMBL; AK011522; BAB27676.1; -; mRNA.
DR EMBL; AK042650; BAC31320.1; -; mRNA.
DR EMBL; AK047006; BAC32940.1; -; mRNA.
DR EMBL; BC046234; AAH46234.1; -; mRNA.
DR EMBL; BC059048; AAH59048.1; -; mRNA.
DR CCDS; CCDS35696.1; -.
DR RefSeq; NP_082411.1; NM_028135.2.
DR AlphaFoldDB; Q8C996; -.
DR SMR; Q8C996; -.
DR IntAct; Q8C996; 1.
DR MINT; Q8C996; -.
DR STRING; 10090.ENSMUSP00000140828; -.
DR iPTMnet; Q8C996; -.
DR PhosphoSitePlus; Q8C996; -.
DR EPD; Q8C996; -.
DR MaxQB; Q8C996; -.
DR PaxDb; Q8C996; -.
DR PeptideAtlas; Q8C996; -.
DR PRIDE; Q8C996; -.
DR ProteomicsDB; 258904; -.
DR Antibodypedia; 33555; 40 antibodies from 14 providers.
DR DNASU; 72160; -.
DR Ensembl; ENSMUST00000027585; ENSMUSP00000027585; ENSMUSG00000026347.
DR Ensembl; ENSMUST00000160616; ENSMUSP00000124307; ENSMUSG00000026347.
DR Ensembl; ENSMUST00000185560; ENSMUSP00000140828; ENSMUSG00000026347.
DR GeneID; 72160; -.
DR KEGG; mmu:72160; -.
DR UCSC; uc011wqz.1; mouse.
DR CTD; 81615; -.
DR MGI; MGI:1919410; Tmem163.
DR VEuPathDB; HostDB:ENSMUSG00000026347; -.
DR eggNOG; ENOG502QW7B; Eukaryota.
DR GeneTree; ENSGT00390000001170; -.
DR HOGENOM; CLU_081161_0_0_1; -.
DR InParanoid; Q8C996; -.
DR OMA; IMRYSAS; -.
DR OrthoDB; 1547703at2759; -.
DR PhylomeDB; Q8C996; -.
DR TreeFam; TF330782; -.
DR BioGRID-ORCS; 72160; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Tmem163; mouse.
DR PRO; PR:Q8C996; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8C996; protein.
DR Bgee; ENSMUSG00000026347; Expressed in cardiac atrium and 118 other tissues.
DR Genevisible; Q8C996; MM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0031901; C:early endosome membrane; ISS:UniProtKB.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; IDA:UniProtKB.
DR GO; GO:0097708; C:intracellular vesicle; EXP:MGI.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; EXP:MGI.
DR GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR GO; GO:0099180; P:zinc ion import into synaptic vesicle; ISO:MGI.
DR Gene3D; 1.20.1510.10; -; 1.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR InterPro; IPR026765; Tmem163.
DR PANTHER; PTHR31937; PTHR31937; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Endosome; Membrane; Phosphoprotein;
KW Reference proteome; Synapse; Transmembrane; Transmembrane helix; Zinc.
FT CHAIN 1..288
FT /note="Transmembrane protein 163"
FT /id="PRO_0000278540"
FT TOPO_DOM 1..87
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..115
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 137..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 171..186
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 208..216
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..254
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..288
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..34
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 11
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:A9CMA6"
FT MOD_RES 45
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 54
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 56
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 60
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 21
FT /note="P -> S (in Ref. 1; BAC32940)"
FT /evidence="ECO:0000305"
FT CONFLICT 288
FT /note="E -> K (in Ref. 1; BAB27676)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 288 AA; 31193 MW; 39552DEE746C4221 CRC64;
MEPALGSERR SPPGPGVPRP PPRGHAPSTA APAPSPAPMS SSVQSDEERQ PRISESGQFS
DGLEDRGLLE SSTRLKPHEA QNYRKKALWV SWLSIIVTLA LAVAAFTVSV MRYSASAFGF
AFDAILDVLS SAIVLWRYSN AAAVHSANRE YIACVILGVI FLLSSICIVV KAIHDLSTRL
LPEVDDFLFS VSILSGILCS VLAVLKFMLG KVLTSRALIT DGFNSLVGGV MGFSILLSAE
VFKHNAAVWY LDGSIGVLIG LTIFAYGVKL LIDMVPRVRQ TRHYEMFE
