TM16B_ARATH
ID TM16B_ARATH Reviewed; 116 AA.
AC Q93VV9; Q9LX43;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit PAM16 like 2 {ECO:0000303|PubMed:24194927};
DE Short=AtPAM16 {ECO:0000303|PubMed:24153405};
DE Short=AtPAM16L2 {ECO:0000303|PubMed:24194927};
DE AltName: Full=Presequence translocated-associated motor subunit PAM16 {ECO:0000250|UniProtKB:P42949};
DE AltName: Full=Protein MUTANT SNC1-ENHANCING 5 {ECO:0000303|PubMed:24153405};
DE AltName: Full=Protein THAXTOMIN A RESISTANT 1 {ECO:0000303|PubMed:12897252};
DE Flags: Precursor;
GN Name=PAM16L2 {ECO:0000303|PubMed:24194927};
GN Synonyms=MUSE5 {ECO:0000303|PubMed:24153405},
GN PAM16 {ECO:0000303|PubMed:24153405}, TXR1 {ECO:0000303|PubMed:12897252};
GN OrderedLocusNames=At3g59280 {ECO:0000312|Araport:AT3G59280};
GN ORFNames=F25L23.140 {ECO:0000312|EMBL:CAB91598.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE
RP SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=12897252; DOI=10.1105/tpc.013342;
RA Scheible W.R., Fry B., Kochevenko A., Schindelasch D., Zimmerli L.,
RA Somerville S., Loria R., Somerville C.R.;
RT "An Arabidopsis mutant resistant to thaxtomin A, a cellulose synthesis
RT inhibitor from Streptomyces species.";
RL Plant Cell 15:1781-1794(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23638731; DOI=10.1186/1471-2229-13-76;
RA Tegg R.S., Shabala S.N., Cuin T.A., Davies N.W., Wilson C.R.;
RT "Enhanced resistance to the cellulose biosynthetic inhibitors, thaxtomin A
RT and isoxaben in Arabidopsis thaliana mutants, also provides specific co-
RT resistance to the auxin transport inhibitor, 1-NPA.";
RL BMC Plant Biol. 13:76-76(2013).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=24153405; DOI=10.1038/ncomms3558;
RA Huang Y., Chen X., Liu Y., Roth C., Copeland C., McFarlane H.E., Huang S.,
RA Lipka V., Wiermer M., Li X.;
RT "Mitochondrial AtPAM16 is required for plant survival and the negative
RT regulation of plant immunity.";
RL Nat. Commun. 4:2558-2558(2013).
RN [7]
RP DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, INDUCTION BY HEAT; SALT AND
RP DROUGHT, GENE FAMILY, NOMENCLATURE, AND REVIEW.
RX PubMed=24194927; DOI=10.1371/journal.pone.0078400;
RA Chen X., Ghazanfar B., Khan A.R., Hayat S., Cheng Z.;
RT "Comparative analysis of putative orthologues of mitochondrial import motor
RT subunit: Pam18 and Pam16 in plants.";
RL PLoS ONE 8:E78400-E78400(2013).
CC -!- FUNCTION: Regulates ATP-dependent protein translocation into the
CC mitochondrial matrix (By similarity). Involved in the uptake of
CC thaxtomin, a phytotoxin produced by Streptomyces bacteria, that causes
CC dramatic cell swelling, reduced seedling growth, and inhibition of
CC cellulose synthesis (PubMed:12897252, PubMed:23638731). Modulates polar
CC auxin transport (PubMed:23638731). Involved in importing a negative
CC regulator of plant immunity into mitochondria, thus protecting plants
CC from over-accumulation of reactive oxygen species (ROS) and preventing
CC autoimmunity. Confers sensitivity to virulent pathogens such as the
CC oomycete H.arabidopsidis Noco2 and the bacteria P.syringae pv.
CC maculicola ES4326 (PubMed:24153405). {ECO:0000250|UniProtKB:Q9Y3D7,
CC ECO:0000269|PubMed:12897252, ECO:0000269|PubMed:23638731,
CC ECO:0000269|PubMed:24153405}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:24153405}; Peripheral membrane protein
CC {ECO:0000269|PubMed:24153405}. Cytoplasm {ECO:0000269|PubMed:12897252}.
CC -!- TISSUE SPECIFICITY: Expressed constitutively and ubiquitously, except
CC in root tips, at low levels. {ECO:0000269|PubMed:12897252,
CC ECO:0000269|PubMed:24194927}.
CC -!- DEVELOPMENTAL STAGE: Accumulates during senescence.
CC {ECO:0000269|PubMed:24194927}.
CC -!- INDUCTION: Induced by heat, salt and drought stresses.
CC {ECO:0000269|PubMed:24194927}.
CC -!- DISRUPTION PHENOTYPE: Reduced growth, especially at high temperatures
CC (>21 degrees Celsius), as well as warped and twisted leaves at the
CC later rosette stage (PubMed:12897252, PubMed:23638731,
CC PubMed:24153405). Increased resistance to thaxtomin, a phytotoxin
CC produced by Streptomyces bacteria, due to reduced toxin uptake
CC (PubMed:12897252). Enhanced tolerance to 1-napthylphthalamic acid
CC (NPA), an auxin efflux transport inhibitor which blocks polar auxin
CC transport. Increased tolerance to isoxaben (IXB), a cellulose
CC biosynthesis inhibitor (CBI) (PubMed:23638731). In single mutants,
CC enhanced resistance against virulent pathogens (e.g. oomycete
CC H.arabidopsidis Noco2 and bacteria P.syringae pv. maculicola ES4326)
CC associated with elevated reactive oxygen species (ROS) accumulation and
CC higher expression of pathogenesis related genes PR-1 and PR-2
CC (PubMed:24153405). The double mutant Atpam16-1 Atpam16l is lethal
CC (PubMed:24153405). {ECO:0000269|PubMed:12897252,
CC ECO:0000269|PubMed:23638731, ECO:0000269|PubMed:24153405}.
CC -!- SIMILARITY: Belongs to the TIM16/PAM16 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB91598.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY112727; AAM63549.1; -; Genomic_DNA.
DR EMBL; AL356014; CAB91598.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79899.1; -; Genomic_DNA.
DR EMBL; AF380643; AAK55724.1; -; mRNA.
DR EMBL; AY054136; AAL06797.1; -; mRNA.
DR PIR; T48996; T48996.
DR RefSeq; NP_567078.1; NM_115790.3.
DR AlphaFoldDB; Q93VV9; -.
DR SMR; Q93VV9; -.
DR STRING; 3702.AT3G59280.1; -.
DR PaxDb; Q93VV9; -.
DR PRIDE; Q93VV9; -.
DR EnsemblPlants; AT3G59280.1; AT3G59280.1; AT3G59280.
DR GeneID; 825097; -.
DR Gramene; AT3G59280.1; AT3G59280.1; AT3G59280.
DR KEGG; ath:AT3G59280; -.
DR Araport; AT3G59280; -.
DR TAIR; locus:2081152; AT3G59280.
DR eggNOG; KOG3442; Eukaryota.
DR HOGENOM; CLU_101461_2_0_1; -.
DR InParanoid; Q93VV9; -.
DR OrthoDB; 1640138at2759; -.
DR PhylomeDB; Q93VV9; -.
DR PRO; PR:Q93VV9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q93VV9; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0031348; P:negative regulation of defense response; IMP:TAIR.
DR GO; GO:1900425; P:negative regulation of defense response to bacterium; IMP:UniProtKB.
DR GO; GO:1902289; P:negative regulation of defense response to oomycetes; IMP:UniProtKB.
DR GO; GO:2000378; P:negative regulation of reactive oxygen species metabolic process; IMP:UniProtKB.
DR GO; GO:1902009; P:positive regulation of toxin transport; IMP:UniProtKB.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR GO; GO:2000012; P:regulation of auxin polar transport; IMP:UniProtKB.
DR GO; GO:0002237; P:response to molecule of bacterial origin; IMP:UniProtKB.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR036869; J_dom_sf.
DR InterPro; IPR005341; Tim16.
DR PANTHER; PTHR12388; PTHR12388; 1.
PE 2: Evidence at transcript level;
KW Auxin signaling pathway; Cytoplasm; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Plant defense; Protein transport;
KW Reference proteome; Transit peptide; Translocation; Transport.
FT TRANSIT 1..27
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 28..116
FT /note="Mitochondrial import inner membrane translocase
FT subunit PAM16 like 2"
FT /id="PRO_0000437997"
FT REGION 57..106
FT /note="J-like"
FT /evidence="ECO:0000250|UniProtKB:P42949"
SQ SEQUENCE 116 AA; 12676 MW; 8258B8ACE3C57FE2 CRC64;
MAGRLLANLI VMGSGIIGRA VFQAYRQALA NASKSGVAQE AMQNGVRQAG KAITEQEARQ
ILGVTEKTSW EEILQKYDKL FENNAKAGSF YLQSKVHRAK ECLEVVYRSQ GNGTPS
