TM182_HUMAN
ID TM182_HUMAN Reviewed; 229 AA.
AC Q6ZP80; C9JML7; Q3B7B8; Q53TT9; Q6GMU0; Q8WW45; Q96NR4;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Transmembrane protein 182;
DE Flags: Precursor;
GN Name=TMEM182; ORFNames=UNQ6974/PRO21957;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ARG-223.
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP ARG-223.
RC TISSUE=Cerebellum, and Heart;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3), AND VARIANT
RP ARG-223.
RC TISSUE=Liver, Placenta, and Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Negatively regulates myogenesis and skeletal muscle
CC regeneration via its association with ITGB1 (By similarity). Modulates
CC ITGB1 activation by decreasing ITGB1-LAMB1 interaction and inhibiting
CC ITGB1-mediated intracellular signaling during myogenesis (By
CC similarity). {ECO:0000250|UniProtKB:A0A1D5NY17}.
CC -!- SUBUNIT: Interacts with ITGB1. {ECO:0000250|UniProtKB:A0A1D5NY17}.
CC -!- INTERACTION:
CC Q6ZP80; O00501: CLDN5; NbExp=3; IntAct=EBI-10255122, EBI-18400628;
CC Q6ZP80; Q8TBE3: FNDC9; NbExp=3; IntAct=EBI-10255122, EBI-12142257;
CC Q6ZP80; P48165: GJA8; NbExp=3; IntAct=EBI-10255122, EBI-17458373;
CC Q6ZP80; O15529: GPR42; NbExp=3; IntAct=EBI-10255122, EBI-18076404;
CC Q6ZP80; P26715: KLRC1; NbExp=3; IntAct=EBI-10255122, EBI-9018187;
CC Q6ZP80; Q13021: MALL; NbExp=3; IntAct=EBI-10255122, EBI-750078;
CC Q6ZP80; Q96HJ5: MS4A3; NbExp=5; IntAct=EBI-10255122, EBI-12806656;
CC Q6ZP80; P60201-2: PLP1; NbExp=3; IntAct=EBI-10255122, EBI-12188331;
CC Q6ZP80; Q9BZL3: SMIM3; NbExp=3; IntAct=EBI-10255122, EBI-741850;
CC Q6ZP80; Q96L08: SUSD3; NbExp=3; IntAct=EBI-10255122, EBI-18194029;
CC Q6ZP80; Q8N205: SYNE4; NbExp=3; IntAct=EBI-10255122, EBI-7131783;
CC Q6ZP80; Q9NV12: TMEM140; NbExp=3; IntAct=EBI-10255122, EBI-2844246;
CC Q6ZP80; A2RU14: TMEM218; NbExp=3; IntAct=EBI-10255122, EBI-10173151;
CC Q6ZP80; O95859: TSPAN12; NbExp=3; IntAct=EBI-10255122, EBI-2466403;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:A0A1D5NY17};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6ZP80-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6ZP80-2; Sequence=VSP_026135;
CC Name=3;
CC IsoId=Q6ZP80-3; Sequence=VSP_026134;
CC -!- SIMILARITY: Belongs to the TMEM182 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH73844.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAI07695.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AY358262; AAQ88629.1; -; mRNA.
DR EMBL; AK054856; BAB70815.1; -; mRNA.
DR EMBL; AK129862; BAC85244.1; -; mRNA.
DR EMBL; AC007251; AAY14911.1; -; Genomic_DNA.
DR EMBL; BC020898; AAH20898.1; -; mRNA.
DR EMBL; BC073844; AAH73844.1; ALT_INIT; mRNA.
DR EMBL; BC107694; AAI07695.1; ALT_INIT; mRNA.
DR EMBL; BC130306; AAI30307.1; -; mRNA.
DR EMBL; BC130308; AAI30309.1; -; mRNA.
DR CCDS; CCDS2064.1; -. [Q6ZP80-1]
DR CCDS; CCDS82490.1; -. [Q6ZP80-2]
DR RefSeq; NP_001308274.1; NM_001321345.1. [Q6ZP80-2]
DR RefSeq; NP_653233.4; NM_144632.4. [Q6ZP80-1]
DR AlphaFoldDB; Q6ZP80; -.
DR BioGRID; 126258; 22.
DR IntAct; Q6ZP80; 15.
DR STRING; 9606.ENSP00000394178; -.
DR GlyGen; Q6ZP80; 2 sites.
DR PhosphoSitePlus; Q6ZP80; -.
DR BioMuta; TMEM182; -.
DR DMDM; 313104025; -.
DR MassIVE; Q6ZP80; -.
DR PaxDb; Q6ZP80; -.
DR PeptideAtlas; Q6ZP80; -.
DR PRIDE; Q6ZP80; -.
DR ProteomicsDB; 68063; -. [Q6ZP80-1]
DR ProteomicsDB; 68064; -. [Q6ZP80-2]
DR Antibodypedia; 66643; 20 antibodies from 14 providers.
DR DNASU; 130827; -.
DR Ensembl; ENST00000409528.5; ENSP00000387258.1; ENSG00000170417.16. [Q6ZP80-2]
DR Ensembl; ENST00000412401.3; ENSP00000394178.2; ENSG00000170417.16. [Q6ZP80-1]
DR GeneID; 130827; -.
DR KEGG; hsa:130827; -.
DR MANE-Select; ENST00000412401.3; ENSP00000394178.2; NM_144632.5; NP_653233.5.
DR UCSC; uc002tcd.5; human. [Q6ZP80-1]
DR CTD; 130827; -.
DR DisGeNET; 130827; -.
DR GeneCards; TMEM182; -.
DR HGNC; HGNC:26391; TMEM182.
DR HPA; ENSG00000170417; Group enriched (heart muscle, skeletal muscle, tongue).
DR neXtProt; NX_Q6ZP80; -.
DR OpenTargets; ENSG00000170417; -.
DR PharmGKB; PA162406075; -.
DR VEuPathDB; HostDB:ENSG00000170417; -.
DR eggNOG; ENOG502QVS4; Eukaryota.
DR GeneTree; ENSGT00390000017581; -.
DR HOGENOM; CLU_146393_0_0_1; -.
DR InParanoid; Q6ZP80; -.
DR OMA; WLLVTEV; -.
DR OrthoDB; 1246131at2759; -.
DR PhylomeDB; Q6ZP80; -.
DR TreeFam; TF331344; -.
DR PathwayCommons; Q6ZP80; -.
DR SignaLink; Q6ZP80; -.
DR BioGRID-ORCS; 130827; 9 hits in 1081 CRISPR screens.
DR ChiTaRS; TMEM182; human.
DR GenomeRNAi; 130827; -.
DR Pharos; Q6ZP80; Tdark.
DR PRO; PR:Q6ZP80; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q6ZP80; protein.
DR Bgee; ENSG00000170417; Expressed in cardiac muscle of right atrium and 145 other tissues.
DR ExpressionAtlas; Q6ZP80; baseline and differential.
DR Genevisible; Q6ZP80; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
DR GO; GO:0014906; P:myotube cell development involved in skeletal muscle regeneration; ISS:UniProtKB.
DR GO; GO:0014908; P:myotube differentiation involved in skeletal muscle regeneration; ISS:UniProtKB.
DR GO; GO:0045662; P:negative regulation of myoblast differentiation; ISS:UniProtKB.
DR GO; GO:1901740; P:negative regulation of myoblast fusion; ISS:UniProtKB.
DR InterPro; IPR004031; PMP22/EMP/MP20/Claudin.
DR InterPro; IPR026763; TMEM182.
DR PANTHER; PTHR32012; PTHR32012; 1.
DR Pfam; PF13903; Claudin_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane; Myogenesis;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..229
FT /note="Transmembrane protein 182"
FT /id="PRO_0000290226"
FT TOPO_DOM 27..114
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..153
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..200
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 222..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 49..59
FT /note="Interaction with ITGB1"
FT /evidence="ECO:0000250|UniProtKB:A0A1D5NY17"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..119
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026134"
FT VAR_SEQ 1..96
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_026135"
FT VARIANT 223
FT /note="W -> R (in dbSNP:rs887987)"
FT /evidence="ECO:0000269|PubMed:12975309,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334"
FT /id="VAR_032773"
SQ SEQUENCE 229 AA; 25879 MW; B96E01B4B28614F1 CRC64;
MRLNIAIFFG ALFGALGVLL FLVAFGSDYW LLATEVGRCS GEKNIENVTF HHEGFFWRCW
FNGIVEENDS NIWKFWYTNQ PPSKNCTHAY LSPYPFMRGE HNSTSYDSAV IYRGFWAVLM
LLGVVAVVIA SFLIICAAPF ASHFLYKAGG GSYIAAGILF SLVVMLYVIW VQAVADMESY
RNMKMKDCLD FTPSVLYGWS FFLAPAGIFF SLLAGLLFLV VGWHIQIHH
