ID   TM186_BOVIN             Reviewed;         212 AA.
AC   Q5EA03;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Transmembrane protein 186 {ECO:0000250|UniProtKB:Q96B77};
GN   Name=TMEM186 {ECO:0000250|UniProtKB:Q96B77};
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Thalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: As part of the MCIA complex, required for efficient assembly
CC       of the mitochondrial complex I. {ECO:0000250|UniProtKB:Q96B77}.
CC   -!- SUBUNIT: Part of the mitochondrial complex I assembly/MCIA complex that
CC       comprises at least the core subunits TMEM126B, NDUFAF1, ECSIT and ACAD9
CC       and complement subunits such as COA1 and TMEM186. Interacts with MT-
CC       ND3. {ECO:0000250|UniProtKB:Q96B77}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q96B77}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q96B77}.
CC   -!- SIMILARITY: Belongs to the TMEM186 family. {ECO:0000305}.
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DR   EMBL; BT020766; AAX08783.1; -; mRNA.
DR   EMBL; BC119850; AAI19851.1; -; mRNA.
DR   RefSeq; NP_001014919.1; NM_001014919.1.
DR   AlphaFoldDB; Q5EA03; -.
DR   STRING; 9913.ENSBTAP00000030481; -.
DR   PaxDb; Q5EA03; -.
DR   Ensembl; ENSBTAT00000030501; ENSBTAP00000030481; ENSBTAG00000022530.
DR   GeneID; 513552; -.
DR   KEGG; bta:513552; -.
DR   CTD; 25880; -.
DR   VEuPathDB; HostDB:ENSBTAG00000022530; -.
DR   VGNC; VGNC:36013; TMEM186.
DR   eggNOG; ENOG502S11D; Eukaryota.
DR   GeneTree; ENSGT00390000000087; -.
DR   HOGENOM; CLU_104872_1_0_1; -.
DR   InParanoid; Q5EA03; -.
DR   OMA; MTIGDTG; -.
DR   OrthoDB; 1296527at2759; -.
DR   TreeFam; TF326623; -.
DR   Reactome; R-BTA-611105; Respiratory electron transport.
DR   Reactome; R-BTA-6799198; Complex I biogenesis.
DR   Proteomes; UP000009136; Chromosome 25.
DR   Bgee; ENSBTAG00000022530; Expressed in choroid plexus and 106 other tissues.
DR   ExpressionAtlas; Q5EA03; baseline.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR   InterPro; IPR026571; Tmem186.
DR   InterPro; IPR045325; TMEM70/TMEM186/TMEM223.
DR   PANTHER; PTHR13603; PTHR13603; 1.
DR   Pfam; PF06979; TMEM70; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..212
FT                   /note="Transmembrane protein 186"
FT                   /id="PRO_0000279438"
FT   TOPO_DOM        1..78
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        100..101
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        123..212
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   212 AA;  23785 MW;  6CCD4F6951C1F12E CRC64;
     MAAVLRAVAR SPGPAAWGRP LHRLCCCGGQ DPRRWVGSGP PHSKEKPLGP ETEKFQMVYR
     FDAIKAFGYL SRLKVAQTAL TVAALPPGLY CYSQGLMPFS SLCLAGGVAG FALAMLCWMS
     HFFRRLVGIL YVNEEGTVLR VAHLTFWGRR QDTYCPVADV IPMTESPDRP QELFMRIQQY
     SGKQTFYLTL RYGRVLDQER FTQVFGVLDA LK