TM186_MOUSE
ID TM186_MOUSE Reviewed; 216 AA.
AC Q9CR76; Q8BHA7; Q8BHT1;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Transmembrane protein 186 {ECO:0000305};
GN Name=Tmem186 {ECO:0000312|MGI:MGI:1913940};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Cecum, Liver, Thymus, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: As part of the MCIA complex, required for efficient assembly
CC of the mitochondrial complex I. {ECO:0000250|UniProtKB:Q96B77}.
CC -!- SUBUNIT: Part of the mitochondrial complex I assembly/MCIA complex that
CC comprises at least the core subunits TMEM126B, NDUFAF1, ECSIT and ACAD9
CC and complement subunits such as COA1 and TMEM186. Interacts with MT-
CC ND3. {ECO:0000250|UniProtKB:Q96B77}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q96B77}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q96B77}.
CC -!- SIMILARITY: Belongs to the TMEM186 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25393.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK009236; BAB26157.2; -; mRNA.
DR EMBL; AK013147; BAC25393.1; ALT_FRAME; mRNA.
DR EMBL; AK014478; BAB29381.2; -; mRNA.
DR EMBL; AK033686; BAC28430.1; -; mRNA.
DR EMBL; AK088656; BAC40482.1; -; mRNA.
DR EMBL; AK159727; BAE35321.1; -; mRNA.
DR EMBL; AK159782; BAE35366.1; -; mRNA.
DR CCDS; CCDS27940.1; -.
DR RefSeq; NP_079984.2; NM_025708.4.
DR AlphaFoldDB; Q9CR76; -.
DR STRING; 10090.ENSMUSP00000053862; -.
DR iPTMnet; Q9CR76; -.
DR PhosphoSitePlus; Q9CR76; -.
DR MaxQB; Q9CR76; -.
DR PaxDb; Q9CR76; -.
DR PRIDE; Q9CR76; -.
DR ProteomicsDB; 259543; -.
DR Antibodypedia; 3062; 44 antibodies from 11 providers.
DR DNASU; 66690; -.
DR Ensembl; ENSMUST00000052505; ENSMUSP00000053862; ENSMUSG00000043140.
DR GeneID; 66690; -.
DR KEGG; mmu:66690; -.
DR UCSC; uc007ycq.1; mouse.
DR CTD; 25880; -.
DR MGI; MGI:1913940; Tmem186.
DR VEuPathDB; HostDB:ENSMUSG00000043140; -.
DR eggNOG; ENOG502S11D; Eukaryota.
DR GeneTree; ENSGT00390000000087; -.
DR HOGENOM; CLU_104872_1_0_1; -.
DR InParanoid; Q9CR76; -.
DR OMA; MTIGDTG; -.
DR OrthoDB; 1296527at2759; -.
DR PhylomeDB; Q9CR76; -.
DR TreeFam; TF326623; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 66690; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Tmem186; mouse.
DR PRO; PR:Q9CR76; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q9CR76; protein.
DR Bgee; ENSMUSG00000043140; Expressed in floor plate of midbrain and 246 other tissues.
DR ExpressionAtlas; Q9CR76; baseline and differential.
DR Genevisible; Q9CR76; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR026571; Tmem186.
DR PANTHER; PTHR13603; PTHR13603; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..216
FT /note="Transmembrane protein 186"
FT /id="PRO_0000279440"
FT TOPO_DOM 1..68
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000305"
FT TRANSMEM 69..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..103
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000305"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..216
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000305"
FT REGION 31..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..52
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 183
FT /note="G -> D (in Ref. 1; BAC25393)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 216 AA; 24632 MW; 3C60B8F1DE5963B3 CRC64;
MAFLLRVVPR LQGPTAWRRP LQGLWCCSGQ GDSKRWVGSR SPHSREKSPG TETETFHTIY
RFRAIRAIGF LSRLKLAQTA VTVVALPPGF YCYSQGLMTL SSLCLLGGVA SFALAMLCWM
SHFFRRLVGI LYVNESGTLL RVAHLTFWGW RQDTYCAVSD MIPLSESQER VQDVFVRIQQ
YSGKQTFYLT LRYGRILDRE RFAQVFGTLA TLKNSK
