TM190_MOUSE
ID TM190_MOUSE Reviewed; 166 AA.
AC Q9D2E9;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Transmembrane protein 190;
DE Flags: Precursor;
GN Name=Tmem190;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=21273369; DOI=10.1530/rep-10-0391;
RA Nishimura H., Gupta S., Myles D., Primakoff P.;
RT "Characterization of mouse sperm TMEM190, a small transmembrane protein
RT with the trefoil domain: evidence for co-localization with IZUMO1 and
RT complex formation with other sperm proteins.";
RL Reproduction 141:437-451(2011).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:21273369}; Single-
CC pass type I membrane protein {ECO:0000269|PubMed:21273369}.
CC Note=Localizes in the intracellular space in testicular germ cells and
CC sperm. Detected also on the cell surface in post-meiotic round
CC spermatids. In cauda epididymal sperm relocates during the acrosome
CC reaction from the inner-acrosomal membrane onto the equatorial segment
CC surface, on which sperm-oocyte fusion occurs. Colocalizes with IZUMO1
CC at the inner-acrosomal membrane.
CC -!- TISSUE SPECIFICITY: Detected in testis and in a mixture of
CC spermatogenic cells at various stages (testicular germ cells). Not
CC detected in heart, brain, spleen, lung, liver, skeletal muscle and
CC kidney. {ECO:0000269|PubMed:21273369}.
CC -!- DISRUPTION PHENOTYPE: Mice are normal with no alterations in fertility,
CC body size and behavior. {ECO:0000269|PubMed:21273369}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK019807; BAB31858.1; -; mRNA.
DR CCDS; CCDS51976.1; -.
DR RefSeq; NP_084304.1; NM_030028.1.
DR AlphaFoldDB; Q9D2E9; -.
DR STRING; 10090.ENSMUSP00000013235; -.
DR iPTMnet; Q9D2E9; -.
DR PhosphoSitePlus; Q9D2E9; -.
DR PaxDb; Q9D2E9; -.
DR PRIDE; Q9D2E9; -.
DR ProteomicsDB; 259468; -.
DR Antibodypedia; 19561; 18 antibodies from 14 providers.
DR Ensembl; ENSMUST00000013235; ENSMUSP00000013235; ENSMUSG00000013091.
DR GeneID; 78052; -.
DR KEGG; mmu:78052; -.
DR UCSC; uc009eyp.2; mouse.
DR CTD; 147744; -.
DR MGI; MGI:1925302; Tmem190.
DR VEuPathDB; HostDB:ENSMUSG00000013091; -.
DR eggNOG; ENOG502T0Q7; Eukaryota.
DR GeneTree; ENSGT00390000006710; -.
DR HOGENOM; CLU_130475_0_0_1; -.
DR InParanoid; Q9D2E9; -.
DR OMA; SSICLFW; -.
DR OrthoDB; 1323569at2759; -.
DR PhylomeDB; Q9D2E9; -.
DR TreeFam; TF337652; -.
DR BioGRID-ORCS; 78052; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q9D2E9; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9D2E9; protein.
DR Bgee; ENSMUSG00000013091; Expressed in spermatid and 42 other tissues.
DR Genevisible; Q9D2E9; MM.
DR GO; GO:0002079; C:inner acrosomal membrane; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043621; F:protein self-association; IDA:MGI.
DR GO; GO:0002244; P:hematopoietic progenitor cell differentiation; IMP:MGI.
DR InterPro; IPR044913; P_trefoil_dom_sf.
DR InterPro; IPR028248; TMEM190.
DR PANTHER; PTHR37868; PTHR37868; 1.
DR Pfam; PF15431; TMEM190; 1.
DR SUPFAM; SSF57492; SSF57492; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..166
FT /note="Transmembrane protein 190"
FT /id="PRO_0000312283"
FT TOPO_DOM 22..81
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 31..71
FT /note="P-type"
FT REGION 130..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 130..148
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 33..61
FT /evidence="ECO:0000250"
FT DISULFID 43..60
FT /evidence="ECO:0000250"
FT DISULFID 55..67
FT /evidence="ECO:0000250"
SQ SEQUENCE 166 AA; 18491 MW; 6B9C75DA1ECA65DC CRC64;
MVGSGISALG LLLLMQGSVD ANGIQGFFYP WSCEGDVWDR ESCGGQAAIE NPNLCLRLRC
CYRDGVCYHQ RPDENMRRKH MWALGWTCGS LLFLITSICL FWWARRQDML HLPRFLHRKC
SKLSKTVSSL SKDRRSANKS TTVLQSPGGE VETAAAVSGE DTGGEE
