TM198_XENTR
ID TM198_XENTR Reviewed; 342 AA.
AC Q6DFQ7; F6TVU3;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Transmembrane protein 198;
GN Name=tmem198;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20431018; DOI=10.1126/science.1183670;
RA Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT "The genome of the Western clawed frog Xenopus tropicalis.";
RL Science 328:633-636(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, INTERACTION WITH LRP6 AND CASEIN KINASES, DEVELOPMENTAL STAGE,
RP DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND MUTAGENESIS OF THR-168;
RP SER-171; THR-172 AND THR-174.
RX PubMed=21536646; DOI=10.1128/mcb.05103-11;
RA Liang J., Fu Y., Cruciat C.M., Jia S., Wang Y., Tong Z., Tao Q.,
RA Ingelfinger D., Boutros M., Meng A., Niehrs C., Wu W.;
RT "Transmembrane protein 198 promotes LRP6 phosphorylation and Wnt signaling
RT activation.";
RL Mol. Cell. Biol. 31:2577-2590(2011).
CC -!- FUNCTION: Promotes lrp6 phosphorylation by casein kinases and thereby
CC plays a role in Wnt signaling. May be a membrane scaffold protein
CC involved in the self-aggregation of lrp6 further enhancing its
CC activity. Required for neural crest formation.
CC {ECO:0000269|PubMed:21536646}.
CC -!- SUBUNIT: Interacts with lrp6. Interacts with casein kinases.
CC {ECO:0000269|PubMed:21536646}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Cell membrane {ECO:0000269|PubMed:21536646}.
CC Cytoplasmic vesicle {ECO:0000269|PubMed:21536646}. Note=Largely located
CC to vesicle-like structures.
CC -!- DEVELOPMENTAL STAGE: Expression widely distributed both maternally and
CC zygotically. During gastrulation, strong expression in the posterior
CC end near the blastopore. {ECO:0000269|PubMed:21536646}.
CC -!- DISRUPTION PHENOTYPE: Morphants have defects in neural crest formation.
CC At the tadpole stage, animals are often less pigmented with ventrally
CC bent tails. Embryos develop smaller eyes and forebrain structures.
CC {ECO:0000269|PubMed:21536646}.
CC -!- SIMILARITY: Belongs to the TMEM198 family. {ECO:0000305}.
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DR EMBL; AAMC01034906; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAMC01034907; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAMC01034908; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC076677; AAH76677.1; -; mRNA.
DR RefSeq; NP_001005013.2; NM_001005013.2.
DR AlphaFoldDB; Q6DFQ7; -.
DR STRING; 8364.ENSXETP00000047267; -.
DR PaxDb; Q6DFQ7; -.
DR DNASU; 448513; -.
DR GeneID; 448513; -.
DR KEGG; xtr:448513; -.
DR CTD; 130612; -.
DR Xenbase; XB-GENE-5751426; tmem198.
DR eggNOG; ENOG502QS1E; Eukaryota.
DR HOGENOM; CLU_043600_0_0_1; -.
DR InParanoid; Q6DFQ7; -.
DR OrthoDB; 1509246at2759; -.
DR TreeFam; TF323324; -.
DR Proteomes; UP000008143; Chromosome 2.
DR Proteomes; UP000790000; Unplaced.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0012506; C:vesicle membrane; IDA:UniProtKB.
DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IEA:InterPro.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR025256; DUF4203.
DR InterPro; IPR040236; TMEM198.
DR PANTHER; PTHR31247; PTHR31247; 1.
DR Pfam; PF13886; DUF4203; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasmic vesicle; Developmental protein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix;
KW Wnt signaling pathway.
FT CHAIN 1..342
FT /note="Transmembrane protein 198"
FT /id="PRO_0000422272"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 168
FT /note="T->P: Very weak enhancement of lrp6 phosphorylation;
FT when associated with A-171; A-172 and R-174."
FT /evidence="ECO:0000269|PubMed:21536646"
FT MUTAGEN 171
FT /note="S->A: Very weak enhancement of lrp6 phosphorylation;
FT when associated with P-168; A-172 and R-174."
FT /evidence="ECO:0000269|PubMed:21536646"
FT MUTAGEN 172
FT /note="T->A: Very weak enhancement of lrp6 phosphorylation;
FT when associated with P-168; A-171 and R-174."
FT /evidence="ECO:0000269|PubMed:21536646"
FT MUTAGEN 174
FT /note="T->R: Very weak enhancement of lrp6 phosphorylation;
FT when associated with P-168; A-171 and A-172."
FT /evidence="ECO:0000269|PubMed:21536646"
SQ SEQUENCE 342 AA; 38179 MW; 3238880E54ADEC79 CRC64;
MLQNIDNRTV DTGQDDPFDG FCSLESERHY DVVPTVVCSL CCLFGLVYTF FGYRCFKAIM
FLSGLLAGSA VIFLLCYKER IMDTQLSLEL SAGIALGIGL LCGLVTMLVH SVGLFMTGLL
LGLLLAIASL VGLEQFYHPP SAWIPVGLMM GSAMLFAVLT LQWQKLFTVV STATFGAAIL
TVCTDYFIEL MLLVQYVYDR LRLETSHPLC WYSWVILGMW PVLSVLGIVV QWKLTAEGFS
HTDVIISRRQ KRLQLLRIRQ KDAKKRQNVA SQEGTYRRKA NPMKRYTGDI LAPSYLQSLR
ERQTGTGTSM SSLSTNMQTI VDMDYECGST VPLTATTPVI HV
