TM199_MOUSE
ID TM199_MOUSE Reviewed; 208 AA.
AC Q5SYH2;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Transmembrane protein 199;
GN Name=Tmem199;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Accessory component of the proton-transporting vacuolar (V)-
CC ATPase protein pump involved in intracellular iron homeostasis. In
CC aerobic conditions, required for intracellular iron homeostasis, thus
CC triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and
CC leading to HIF1A hydroxylation and subsequent proteasomal degradation.
CC Necessary for endolysosomal acidification and lysosomal degradation (By
CC similarity). May be involved in Golgi homeostasis (By similarity).
CC {ECO:0000250|UniProtKB:Q8N511}.
CC -!- SUBUNIT: Accessory component of the multisubunit proton-transporting
CC vacuolar (V)-ATPase protein pump. {ECO:0000250|UniProtKB:Q8N511}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, COPI-coated vesicle membrane
CC {ECO:0000250|UniProtKB:Q8N511}; Multi-pass membrane protein
CC {ECO:0000255}. Endoplasmic reticulum-Golgi intermediate compartment
CC membrane {ECO:0000250|UniProtKB:Q8N511}; Multi-pass membrane protein
CC {ECO:0000255}. Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q8N511}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Partial colocalization with GOLGB1.
CC {ECO:0000250|UniProtKB:Q8N511}.
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DR EMBL; AL591177; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS25108.1; -.
DR RefSeq; NP_954669.2; NM_199199.3.
DR AlphaFoldDB; Q5SYH2; -.
DR BioGRID; 228816; 2.
DR STRING; 10090.ENSMUSP00000058599; -.
DR iPTMnet; Q5SYH2; -.
DR PhosphoSitePlus; Q5SYH2; -.
DR EPD; Q5SYH2; -.
DR MaxQB; Q5SYH2; -.
DR PaxDb; Q5SYH2; -.
DR PRIDE; Q5SYH2; -.
DR ProteomicsDB; 260685; -.
DR TopDownProteomics; Q5SYH2; -.
DR Antibodypedia; 34886; 41 antibodies from 20 providers.
DR Ensembl; ENSMUST00000052566; ENSMUSP00000058599; ENSMUSG00000051232.
DR GeneID; 195040; -.
DR KEGG; mmu:195040; -.
DR UCSC; uc007kjn.1; mouse.
DR CTD; 147007; -.
DR MGI; MGI:2144113; Tmem199.
DR VEuPathDB; HostDB:ENSMUSG00000051232; -.
DR eggNOG; ENOG502RXKD; Eukaryota.
DR GeneTree; ENSGT00390000014591; -.
DR HOGENOM; CLU_114590_0_0_1; -.
DR InParanoid; Q5SYH2; -.
DR OMA; DFGQQVR; -.
DR OrthoDB; 1216090at2759; -.
DR PhylomeDB; Q5SYH2; -.
DR TreeFam; TF314610; -.
DR BioGRID-ORCS; 195040; 20 hits in 74 CRISPR screens.
DR ChiTaRS; Tmem199; mouse.
DR PRO; PR:Q5SYH2; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5SYH2; protein.
DR Bgee; ENSMUSG00000051232; Expressed in spermatocyte and 260 other tissues.
DR ExpressionAtlas; Q5SYH2; baseline and differential.
DR Genevisible; Q5SYH2; MM.
DR GO; GO:0030663; C:COPI-coated vesicle membrane; ISS:UniProtKB.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005764; C:lysosome; IEA:GOC.
DR GO; GO:0016471; C:vacuolar proton-transporting V-type ATPase complex; ISS:UniProtKB.
DR GO; GO:0006879; P:cellular iron ion homeostasis; ISS:UniProtKB.
DR GO; GO:0036295; P:cellular response to increased oxygen levels; ISS:UniProtKB.
DR GO; GO:0007042; P:lysosomal lumen acidification; ISS:UniProtKB.
DR GO; GO:1905146; P:lysosomal protein catabolic process; ISS:UniProtKB.
DR GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IEA:InterPro.
DR InterPro; IPR021013; ATPase_Vma12.
DR PANTHER; PTHR31394; PTHR31394; 1.
DR Pfam; PF11712; Vma12; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasmic vesicle; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q8N511"
FT CHAIN 2..208
FT /note="Transmembrane protein 199"
FT /id="PRO_0000079299"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q8N511"
SQ SEQUENCE 208 AA; 23099 MW; F86D10F4FB56BA2B CRC64;
MASSLLAGER LVRALGPGGE LEREQLPRKL RAQLEAALGK KHAGSDNATG PRRLVSFRLI
RDLHQHLRER NSRLYLHELL EGSDIYFPEI VKPPRNPELV ARLEKIKIQL ANEEYKRITR
NVTCQDAQCG GTLSDLGKQV RSVKALVVTI FNFIITVAAA FVCTYLGSQY VFTEMASRVL
AALIVASVVG LAELYVMVRA MEGELGEL
