TM1S_SOLLC
ID TM1S_SOLLC Reviewed; 754 AA.
AC A7M6E8;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=ToMV susceptible protein tm-1(GCR26) {ECO:0000303|PubMed:17699618};
DE AltName: Full=Disease susceptible protein tm-1 {ECO:0000305};
DE AltName: Full=Protein p80(GCR26) {ECO:0000303|PubMed:17699618};
GN Name=tm-1 {ECO:0000303|PubMed:17699618};
GN OrderedLocusNames=Solyc02g062560 {ECO:0000305};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Craigella GCR26;
RX PubMed=17699618; DOI=10.1073/pnas.0703203104;
RA Ishibashi K., Masuda K., Naito S., Meshi T., Ishikawa M.;
RT "An inhibitor of viral RNA replication is encoded by a plant resistance
RT gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:13833-13838(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Heinz 1706;
RX PubMed=22660326; DOI=10.1038/nature11119;
RG Tomato Genome Consortium;
RT "The tomato genome sequence provides insights into fleshy fruit
RT evolution.";
RL Nature 485:635-641(2012).
RN [3]
RP FUNCTION.
RX PubMed=3686829; DOI=10.1016/0042-6822(87)90147-4;
RA Watanabe Y., Kishibayashi N., Motoyoshi F., Okada Y.;
RT "Characterization of Tm-1 gene action on replication of common isolates and
RT a resistance-breaking isolate of TMV.";
RL Virology 161:527-532(1987).
RN [4]
RP GENE FAMILY.
RX PubMed=24710868; DOI=10.1007/s001220050745;
RA Ohmori T., Murata M., Motoyoshi F.;
RT "Characterization of disease resistance gene-like sequences in near-
RT isogenic lines of tomato.";
RL Theor. Appl. Genet. 96:331-338(1998).
RN [5]
RP FUNCTION.
RC STRAIN=cv. Craigella GCR26;
RX PubMed=17238011; DOI=10.1007/s00705-006-0915-8;
RA Strasser M., Pfitzner A.J.P.;
RT "The double-resistance-breaking Tomato mosaic virus strain ToMV1-2 contains
RT two independent single resistance-breaking domains.";
RL Arch. Virol. 152:903-914(2007).
RN [6]
RP FUNCTION, MISCELLANEOUS, SUBUNIT, AND INTERACTION WITH TOBAMOVIRUSES
RP REPLICATION PROTEINS (MICROBIAL INFECTION).
RX PubMed=19423673; DOI=10.1073/pnas.0809105106;
RA Ishibashi K., Naito S., Meshi T., Ishikawa M.;
RT "An inhibitory interaction between viral and cellular proteins underlies
RT the resistance of tomato to nonadapted tobamoviruses.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:8778-8783(2009).
RN [7]
RP FUNCTION.
RX PubMed=24299004; DOI=10.1111/mpp.12107;
RA Mizumoto H., Morikawa Y., Ishibashi K., Kimura K., Matsumoto K.,
RA Tokunaga M., Kiba A., Ishikawa M., Okuno T., Hikichi Y.;
RT "Functional characterization of the mutations in Pepper mild mottle virus
RT overcoming tomato tm-1-mediated resistance.";
RL Mol. Plant Pathol. 15:479-487(2014).
CC -!- FUNCTION: Inhibitor of viral RNA replication which confers resistance
CC to some tobamoviruses including tobacco mild green mosaic virus (TMGMV)
CC and pepper mild mottle virus (PMMoV), but not to tomato mosaic virus
CC (ToMV strains L, ToMV0 and ToMV1-2) and tobacco mosaic virus (TMV)
CC (PubMed:17699618, PubMed:3686829, PubMed:17238011, PubMed:19423673,
CC PubMed:24299004). Prevents tobamoviruses RNA replication by affecting
CC the association of tobamoviruses replication proteins (large and small
CC subunits) with host membrane-associated proteins (e.g. TOM1, TOM2A and
CC ARL8), thus inhibiting the replication complex formation on the
CC membranes and avoiding viral negative-strand RNA synthesis
CC (PubMed:19423673). {ECO:0000269|PubMed:17238011,
CC ECO:0000269|PubMed:17699618, ECO:0000269|PubMed:19423673,
CC ECO:0000269|PubMed:24299004, ECO:0000269|PubMed:3686829}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:A7M6E7}.
CC -!- SUBUNIT: (Microbial infection) Binds, via an ATP bridge, to the
CC tobamoviruses avirulent (Avr) replication proteins (large and small
CC subunits, e.g. tobacco mild green mosaic virus (TMGMV) AC P18339 and
CC pepper mild mottle virus (PMMoV) AC P89657) to inhibit their function
CC after the translation of tobamoviruses RNA, but before the viral
CC replication complex formation on the membrane surfaces; this
CC interaction is not possible with resistance-breaking strains
CC replication proteins. {ECO:0000269|PubMed:19423673}.
CC -!- MISCELLANEOUS: The Tm-1 allele present in the tomato mosaic virus
CC (ToMV)-resistant tomato cv. Craigella isolate GCR237 (AC A7M6E7)
CC confers resistance to ToMV but not the allele present in the ToMV-
CC susceptible tomato cv. Craigella isolate GCR26 (AC A7M6E8).
CC {ECO:0000269|PubMed:17699618}.
CC -!- MISCELLANEOUS: Transgenic tobacco plants expressing tm-1 exhibit
CC resistance to tobacco mild green mosaic virus (TMGMV) and pepper mild
CC mottle virus (PMMoV), tobamoviruses that cannot multiply in tomato.
CC {ECO:0000269|PubMed:19423673}.
CC -!- SIMILARITY: Belongs to the UPF0261 family. {ECO:0000305}.
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DR EMBL; AB287297; BAF75725.1; -; mRNA.
DR EMBL; CM001065; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001234724.1; NM_001247795.2.
DR RefSeq; XP_010315841.1; XM_010317539.2.
DR RefSeq; XP_019067502.1; XM_019211957.1.
DR AlphaFoldDB; A7M6E8; -.
DR SMR; A7M6E8; -.
DR STRING; 4081.Solyc02g062560.2.1; -.
DR PaxDb; A7M6E8; -.
DR PRIDE; A7M6E8; -.
DR EnsemblPlants; Solyc02g062560.3.1; Solyc02g062560.3.1; Solyc02g062560.3.
DR GeneID; 100134884; -.
DR Gramene; Solyc02g062560.3.1; Solyc02g062560.3.1; Solyc02g062560.3.
DR KEGG; sly:100134884; -.
DR eggNOG; ENOG502QQGA; Eukaryota.
DR HOGENOM; CLU_021337_1_0_1; -.
DR InParanoid; A7M6E8; -.
DR OMA; VDQHELP; -.
DR OrthoDB; 770968at2759; -.
DR PhylomeDB; A7M6E8; -.
DR Proteomes; UP000004994; Chromosome 2.
DR ExpressionAtlas; A7M6E8; baseline and differential.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IDA:UniProtKB.
DR GO; GO:0044830; P:modulation by host of viral RNA genome replication; IDA:UniProtKB.
DR CDD; cd15488; Tm-1-like; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR009215; TIM-br_IGPS-like.
DR InterPro; IPR044122; UPF0261_dom.
DR Pfam; PF09370; PEP_hydrolase; 1.
DR Pfam; PF06792; UPF0261; 1.
DR SUPFAM; SSF51621; SSF51621; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Host-virus interaction; Nucleotide-binding; Plant defense;
KW Reference proteome.
FT CHAIN 1..754
FT /note="ToMV susceptible protein tm-1(GCR26)"
FT /id="PRO_0000448713"
FT REGION 1..201
FT /note="N-terminal inhibitory domain NN"
FT /evidence="ECO:0000250|UniProtKB:A7M6E7"
FT REGION 211..431
FT /note="N-terminal inhibitory domain NC"
FT /evidence="ECO:0000250|UniProtKB:A7M6E7"
FT BINDING 18..20
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:A7M6E7"
FT BINDING 55
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:A7M6E7"
FT BINDING 92
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:A7M6E7"
FT BINDING 124..127
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:A7M6E7"
SQ SEQUENCE 754 AA; 80437 MW; 35410C1744591B02 CRC64;
MATAQSNSPR VFCIGTADTK FDELRFLSEH VRSSLNSFSN KSSFKVGVTV VDVSTSRKET
NSCADFDFVP SKDVLSCYAR GEGTVGRFPD IRGQAIAIMN KALETFLSKA NGEQNLAGVI
GLGGSGGTSL LSSAFRSLPI GIPKVIISTV ASGQTESYIG TSDLVLFPSV VDICGINNVS
KVVLSNAGAA FAGMVIGRLE SSKEHSITNG KFTVGVTMFG VTTPCVNAVK ERLVKEGYET
LVFHATGVGG RAMEDLVRGG FIQGVLDITT TEVADYVVGG VMACDSSRFD AILEKKIPLV
LSVGALDMVN FGPKTTIPPE FQQRKIHQHN EQVSLMHTTV GENKKFAAFI AEKLNKASSS
VCVCLPEKGV SALDAPGKDF YDPEATSCLT HELQMLLENN ERCQVKVYPY HINDVEFANA
LVDSFLEMSP KSGHVECQTA ESKSIQGIQN VNAVLEKYPS CNGKNFSRLN DFPNAKPETL
QKRIVILQKL KDQISKGKPI IGAGAGTGIS AKFEEAGGVD LIVLYNSGRF RMAGRGSLAG
LLPFADANAI VLEMANEVLP VVKEVAVLAG VCATDPFRRM DNFLKQLESV GFCGVQNFPT
VGLFDGNFRQ NLEETGMGYG LEVEMIATAH RMGLLTTPYA FCPDEAVAMA EAGADIIVAH
MGLTTSGSIG AKTAVSLEES VTCVQAIADA THRINPDAIV LCHGGPISSP EEAAYVLKRT
TGVHGFYGAS SMERLPVEQA ITATVQQYKS ISME
