TM203_MOUSE
ID TM203_MOUSE Reviewed; 136 AA.
AC Q8R235; Q8BQ59;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Transmembrane protein 203;
GN Name=Tmem203;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Spinal ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25996873; DOI=10.1371/journal.pone.0127480;
RA Shambharkar P.B., Bittinger M., Latario B., Xiong Z., Bandyopadhyay S.,
RA Davis V., Lin V., Yang Y., Valdez R., Labow M.A.;
RT "TMEM203 is a novel regulator of intracellular calcium homeostasis and is
RT required for spermatogenesis.";
RL PLoS ONE 10:E0127480-E0127480(2015).
CC -!- FUNCTION: Involved in the regulation of cellular calcium homeotasis
CC (PubMed:25996873). Required for spermatogenesis (PubMed:25996873).
CC {ECO:0000269|PubMed:25996873}.
CC -!- SUBUNIT: Interacts with ATP2A2, ITPR3 and STIM1.
CC {ECO:0000250|UniProtKB:Q969S6}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q969S6}; Multi-pass membrane protein
CC {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Male mutants are sterile and exhibit a profound
CC defect in spermatogenesis and spermagiogenesis.
CC {ECO:0000269|PubMed:25996873}.
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DR EMBL; AK051471; BAC34652.1; -; mRNA.
DR EMBL; AL732309; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC022606; AAH22606.1; -; mRNA.
DR CCDS; CCDS38070.1; -.
DR RefSeq; NP_796318.2; NM_177344.3.
DR AlphaFoldDB; Q8R235; -.
DR STRING; 10090.ENSMUSP00000100614; -.
DR PaxDb; Q8R235; -.
DR PRIDE; Q8R235; -.
DR Antibodypedia; 64518; 2 antibodies from 2 providers.
DR DNASU; 227615; -.
DR Ensembl; ENSMUST00000104998; ENSMUSP00000100614; ENSMUSG00000078201.
DR GeneID; 227615; -.
DR KEGG; mmu:227615; -.
DR UCSC; uc008iqz.1; mouse.
DR CTD; 94107; -.
DR MGI; MGI:2443597; Tmem203.
DR VEuPathDB; HostDB:ENSMUSG00000078201; -.
DR eggNOG; KOG3631; Eukaryota.
DR GeneTree; ENSGT00510000049510; -.
DR HOGENOM; CLU_145619_1_0_1; -.
DR InParanoid; Q8R235; -.
DR OMA; FEIWINL; -.
DR OrthoDB; 1407976at2759; -.
DR PhylomeDB; Q8R235; -.
DR TreeFam; TF323514; -.
DR BioGRID-ORCS; 227615; 7 hits in 73 CRISPR screens.
DR PRO; PR:Q8R235; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q8R235; protein.
DR Bgee; ENSMUSG00000078201; Expressed in embryonic brain and 187 other tissues.
DR Genevisible; Q8R235; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR InterPro; IPR019396; TM_Fragile-X-F-assoc.
DR PANTHER; PTHR13568; PTHR13568; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..136
FT /note="Transmembrane protein 203"
FT /id="PRO_0000317204"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 118
FT /note="T -> K (in Ref. 1; BAC34652)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 136 AA; 15776 MW; 76D92BB2935E6093 CRC64;
MLFSLRELVQ WLGFATFEIF VHLLALLVFS VLLALRVDGL TPGLSWWNVF VPFFAADGLS
TYFTTIVSVR LFQDGEKRLA VLRLFWVLTV LSLKFVFEML LCQKLVEQTR ELWFGLITSP
VFILLQLLMI RACRVN
