TM214_HUMAN
ID TM214_HUMAN Reviewed; 689 AA.
AC Q6NUQ4; A6NNF2; B3KUI9; B5MCD8; D6W547; Q53SW1; Q69YH4; Q8NC45; Q8WZ37;
AC Q9NXH2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Transmembrane protein 214;
GN Name=TMEM214; ORFNames=PP446;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Colon mucosa, and Stomach;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-689 (ISOFORM 1).
RC TISSUE=Amygdala, and Stomach;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 286-689 (ISOFORM 1).
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=23661706; DOI=10.1074/jbc.m113.458836;
RA Li C., Wei J., Li Y., He X., Zhou Q., Yan J., Zhang J., Liu Y., Liu Y.,
RA Shu H.B.;
RT "Transmembrane protein 214 (TMEM214) mediates endoplasmic reticulum stress-
RT induced caspase 4 enzyme activation and apoptosis.";
RL J. Biol. Chem. 288:17908-17917(2013).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Critical mediator, in cooperation with CASP4, of endoplasmic
CC reticulum-stress induced apoptosis. Required or the activation of CASP4
CC following endoplasmic reticulum stress. {ECO:0000269|PubMed:23661706}.
CC -!- SUBUNIT: Constitutively interacts with CASP4; required for the
CC localization of procaspase 4 to the ER.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:23661706}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:23661706}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6NUQ4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6NUQ4-2; Sequence=VSP_041155;
CC -!- SIMILARITY: Belongs to the TMEM214 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL55739.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA91038.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAC11331.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAH10591.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK000261; BAA91038.1; ALT_INIT; mRNA.
DR EMBL; AK074981; BAC11331.1; ALT_FRAME; mRNA.
DR EMBL; AK097308; BAG53451.1; -; mRNA.
DR EMBL; AC013472; AAY14654.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00655.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00657.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00659.1; -; Genomic_DNA.
DR EMBL; AL833725; CAH10591.1; ALT_INIT; mRNA.
DR EMBL; BC068479; AAH68479.1; -; mRNA.
DR EMBL; AF289555; AAL55739.1; ALT_INIT; mRNA.
DR CCDS; CCDS42664.1; -. [Q6NUQ4-1]
DR CCDS; CCDS46242.1; -. [Q6NUQ4-2]
DR RefSeq; NP_001077059.1; NM_001083590.1. [Q6NUQ4-2]
DR RefSeq; NP_060197.4; NM_017727.4. [Q6NUQ4-1]
DR AlphaFoldDB; Q6NUQ4; -.
DR BioGRID; 120216; 163.
DR IntAct; Q6NUQ4; 52.
DR MINT; Q6NUQ4; -.
DR STRING; 9606.ENSP00000238788; -.
DR GlyGen; Q6NUQ4; 3 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q6NUQ4; -.
DR PhosphoSitePlus; Q6NUQ4; -.
DR SwissPalm; Q6NUQ4; -.
DR BioMuta; TMEM214; -.
DR DMDM; 189042272; -.
DR EPD; Q6NUQ4; -.
DR jPOST; Q6NUQ4; -.
DR MassIVE; Q6NUQ4; -.
DR MaxQB; Q6NUQ4; -.
DR PaxDb; Q6NUQ4; -.
DR PeptideAtlas; Q6NUQ4; -.
DR PRIDE; Q6NUQ4; -.
DR ProteomicsDB; 66702; -. [Q6NUQ4-1]
DR ProteomicsDB; 66703; -. [Q6NUQ4-2]
DR Antibodypedia; 28071; 126 antibodies from 26 providers.
DR DNASU; 54867; -.
DR Ensembl; ENST00000238788.14; ENSP00000238788.9; ENSG00000119777.20. [Q6NUQ4-1]
DR Ensembl; ENST00000321326.11; ENSP00000319469.7; ENSG00000119777.20. [Q6NUQ4-1]
DR Ensembl; ENST00000404032.7; ENSP00000384417.3; ENSG00000119777.20. [Q6NUQ4-2]
DR GeneID; 54867; -.
DR KEGG; hsa:54867; -.
DR MANE-Select; ENST00000238788.14; ENSP00000238788.9; NM_017727.5; NP_060197.4.
DR UCSC; uc002ria.5; human. [Q6NUQ4-1]
DR CTD; 54867; -.
DR DisGeNET; 54867; -.
DR GeneCards; TMEM214; -.
DR HGNC; HGNC:25983; TMEM214.
DR HPA; ENSG00000119777; Low tissue specificity.
DR MIM; 615301; gene.
DR neXtProt; NX_Q6NUQ4; -.
DR OpenTargets; ENSG00000119777; -.
DR PharmGKB; PA162406505; -.
DR VEuPathDB; HostDB:ENSG00000119777; -.
DR eggNOG; KOG4467; Eukaryota.
DR GeneTree; ENSGT00390000002693; -.
DR HOGENOM; CLU_025330_1_0_1; -.
DR InParanoid; Q6NUQ4; -.
DR OMA; IGQNWPS; -.
DR OrthoDB; 1409017at2759; -.
DR PhylomeDB; Q6NUQ4; -.
DR TreeFam; TF329489; -.
DR PathwayCommons; Q6NUQ4; -.
DR SignaLink; Q6NUQ4; -.
DR BioGRID-ORCS; 54867; 12 hits in 1084 CRISPR screens.
DR ChiTaRS; TMEM214; human.
DR GenomeRNAi; 54867; -.
DR Pharos; Q6NUQ4; Tdark.
DR PRO; PR:Q6NUQ4; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q6NUQ4; protein.
DR Bgee; ENSG00000119777; Expressed in adenohypophysis and 204 other tissues.
DR ExpressionAtlas; Q6NUQ4; baseline and differential.
DR Genevisible; Q6NUQ4; HS.
DR GO; GO:0005881; C:cytoplasmic microtubule; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:HPA.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IDA:HPA.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR InterPro; IPR019308; TMEM214.
DR PANTHER; PTHR13448; PTHR13448; 1.
DR Pfam; PF10151; TMEM214; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Apoptosis; Endoplasmic reticulum;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..689
FT /note="Transmembrane protein 214"
FT /id="PRO_0000321897"
FT TRANSMEM 480..500
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 616..636
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 70..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CARBOHYD 269
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 307
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 168..212
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_041155"
FT VARIANT 351
FT /note="V -> M (in dbSNP:rs1124649)"
FT /id="VAR_039370"
FT CONFLICT 99
FT /note="P -> H (in Ref. 4; AAH68479)"
FT /evidence="ECO:0000305"
FT CONFLICT 138
FT /note="P -> Q (in Ref. 4; AAH68479)"
FT /evidence="ECO:0000305"
FT CONFLICT 326
FT /note="F -> Y (in Ref. 1; BAA91038)"
FT /evidence="ECO:0000305"
FT CONFLICT 502
FT /note="R -> W (in Ref. 6; AAL55739)"
FT /evidence="ECO:0000305"
FT CONFLICT 651
FT /note="V -> A (in Ref. 1; BAG53451)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 689 AA; 77151 MW; A12259676BA5B8E4 CRC64;
MATKTAGVGR WEVVKKGRRP GVGAGAGGRG GGRNRRALGE ANGVWKYDLT PAIQTTSTLY
ERGFENIMKR QNKEQVPPPA VEPKKPGNKK QPKKVATPPN QNQKQGRFRS LEEALKALDV
ADLQKELDKS QSVFSGNPSI WLKDLASYLN YKLQAPLSEP TLSQHTHDYP YSLVSRELRG
IIRGLLAKAA GSLELFFDHC LFTMLQELDK TPGESLHGYR ICIQAILQDK PKIATANLGK
FLELLRSHQS RPAKCLTIMW ALGQAGFANL TEGLKVWLGI MLPVLGIKSL SPFAITYLDR
LLLMHPNLTK GFGMIGPKDF FPLLDFAYMP NNSLTPSLQE QLCQLYPRLK VLAFGAKPDS
TLHTYFPSFL SRATPSCPPE MKKELLSSLT ECLTVDPLSA SVWRQLYPKH LSQSSLLLEH
LLSSWEQIPK KVQKSLQETI QSLKLTNQEL LRKGSSNNQD VVTCDMACKG LLQQVQGPRL
PWTRLLLLLL VFAVGFLCHD LRSHSSFQAS LTGRLLRSSG FLPASQQACA KLYSYSLQGY
SWLGETLPLW GSHLLTVVRP SLQLAWAHTN ATVSFLSAHC ASHLAWFGDS LTSLSQRLQI
QLPDSVNQLL RYLRELPLLF HQNVLLPLWH LLLEALAWAQ EHCHEACRGE VTWDCMKTQL
SEAVHWTWLC LQDITVAFLD WALALISQQ
