TM216_RAT
ID TM216_RAT Reviewed; 141 AA.
AC B6ID01; D4A451;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=Transmembrane protein 216;
GN Name=Tmem216;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Pituitary, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Part of the tectonic-like complex which is required for
CC tissue-specific ciliogenesis and may regulate ciliary membrane
CC composition. {ECO:0000250}.
CC -!- SUBUNIT: Part of the tectonic-like complex (also named B9 complex).
CC Interacts with TMEM107. {ECO:0000250|UniProtKB:Q9P0N5}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Cytoplasm, cytoskeleton, cilium basal body
CC {ECO:0000250}. Note=Localizes at the transition zone, a region between
CC the basal body and the ciliary axoneme. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=B6ID01-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B6ID01-2; Sequence=VSP_040298;
CC Name=3;
CC IsoId=B6ID01-3; Sequence=VSP_040298, VSP_040299;
CC -!- MISCELLANEOUS: [Isoform 3]: Due to intron retention. {ECO:0000305}.
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DR EMBL; CH473953; EDM12818.1; -; Genomic_DNA.
DR EMBL; CH473953; EDM12819.1; -; Genomic_DNA.
DR EMBL; CH473953; EDM12820.1; -; Genomic_DNA.
DR EMBL; CH473953; EDM12821.1; -; Genomic_DNA.
DR EMBL; BC168207; AAI68207.1; -; mRNA.
DR EMBL; CB568391; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001257968.1; NM_001271039.1. [B6ID01-1]
DR RefSeq; XP_006231107.1; XM_006231045.3. [B6ID01-2]
DR AlphaFoldDB; B6ID01; -.
DR STRING; 10116.ENSRNOP00000028079; -.
DR PaxDb; B6ID01; -.
DR GeneID; 361727; -.
DR KEGG; rno:361727; -.
DR CTD; 51259; -.
DR RGD; 1304607; Tmem216.
DR VEuPathDB; HostDB:ENSRNOG00000020692; -.
DR eggNOG; KOG4502; Eukaryota.
DR HOGENOM; CLU_135948_0_0_1; -.
DR InParanoid; B6ID01; -.
DR OMA; QFPWGLK; -.
DR OrthoDB; 1584392at2759; -.
DR PhylomeDB; B6ID01; -.
DR Reactome; R-RNO-5620912; Anchoring of the basal body to the plasma membrane.
DR PRO; PR:B6ID01; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Proteomes; UP000234681; Chromosome 1.
DR Bgee; ENSRNOG00000020692; Expressed in ovary and 20 other tissues.
DR Genevisible; B6ID01; RN.
DR GO; GO:0035869; C:ciliary transition zone; ISO:RGD.
DR GO; GO:0005929; C:cilium; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0036038; C:MKS complex; ISS:UniProtKB.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:1905515; P:non-motile cilium assembly; IBA:GO_Central.
DR InterPro; IPR019184; Uncharacterised_TM-17.
DR PANTHER; PTHR13531; PTHR13531; 1.
DR Pfam; PF09799; Transmemb_17; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell projection; Cilium biogenesis/degradation;
KW Cytoplasm; Cytoskeleton; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..141
FT /note="Transmembrane protein 216"
FT /id="PRO_0000402544"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..54
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_040298"
FT VAR_SEQ 137..141
FT /note="SMDRI -> RYNC (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_040299"
FT CONFLICT 28..29
FT /note="FL -> YQ (in Ref. 3; CB568391)"
FT /evidence="ECO:0000305"
FT CONFLICT 40
FT /note="L -> H (in Ref. 3; CB568391)"
FT /evidence="ECO:0000305"
FT CONFLICT 51
FT /note="L -> Q (in Ref. 3; CB568391)"
FT /evidence="ECO:0000305"
FT CONFLICT 95
FT /note="M -> R (in Ref. 3; CB568391)"
FT /evidence="ECO:0000305"
FT CONFLICT 116..120
FT /note="ILLFF -> NLLFI (in Ref. 3; CB568391)"
FT /evidence="ECO:0000305"
FT CONFLICT 127
FT /note="L -> H (in Ref. 3; CB568391)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 141 AA; 16174 MW; 96DCFD262A9F0003 CRC64;
MAPRDKRLSS TPLEILFFLN GWYYATYFLL ELLIFLYKGL LLPYPTANLV LDVVMLLLYL
GIEVIRLFFG TKGNLCQRKM PLGISVALTF PSAMMASYYL LLQTYVLRLE AIMNSILLFF
CGSELLLEML TLATFSSMDR I
