ID   TM221_MOUSE             Reviewed;         230 AA.
AC   Q8K071; B2RWI9;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 2.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Transmembrane protein 221;
GN   Name=Tmem221;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain, and Thymus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; AC162033; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC033932; AAH33932.1; -; mRNA.
DR   EMBL; BC147816; AAI47817.1; -; mRNA.
DR   EMBL; BC147831; AAI47832.1; -; mRNA.
DR   CCDS; CCDS52586.1; -.
DR   RefSeq; NP_001093932.1; NM_001100462.1.
DR   AlphaFoldDB; Q8K071; -.
DR   STRING; 10090.ENSMUSP00000058317; -.
DR   MaxQB; Q8K071; -.
DR   PaxDb; Q8K071; -.
DR   PRIDE; Q8K071; -.
DR   Ensembl; ENSMUST00000052072; ENSMUSP00000058317; ENSMUSG00000043664.
DR   GeneID; 434325; -.
DR   KEGG; mmu:434325; -.
DR   UCSC; uc012gft.1; mouse.
DR   CTD; 100130519; -.
DR   MGI; MGI:3525074; Tmem221.
DR   VEuPathDB; HostDB:ENSMUSG00000043664; -.
DR   eggNOG; ENOG502RTGK; Eukaryota.
DR   GeneTree; ENSGT00390000001907; -.
DR   HOGENOM; CLU_079663_0_0_1; -.
DR   InParanoid; Q8K071; -.
DR   OMA; MTHTLFR; -.
DR   OrthoDB; 1322590at2759; -.
DR   PhylomeDB; Q8K071; -.
DR   TreeFam; TF336085; -.
DR   BioGRID-ORCS; 434325; 3 hits in 70 CRISPR screens.
DR   PRO; PR:Q8K071; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; Q8K071; protein.
DR   Bgee; ENSMUSG00000043664; Expressed in right kidney and 35 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR029201; Jiraiya.
DR   Pfam; PF15038; Jiraiya; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..230
FT                   /note="Transmembrane protein 221"
FT                   /id="PRO_0000332135"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          184..230
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        186..201
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   230 AA;  23954 MW;  7F4C59C9F6AF0BDA CRC64;
     MGRSYGGRVL AAMTLLGIPA AVLVALAAQL LFQLQAGRAE LRRVRTDGLH PELDPDAGLP
     EAAAGALLPL ATALAALAQV LGLSCLLLAA LCGHLGAELA RGPGPGRSDW FLYDCRLLRH
     SALGLFCCGV SVYLAALAIY ALLLFEIEAG AAAASILGSG ALILVAIMTH TLFRAVQATR
     RGLRELSPPS FEDEPARPSE DSKSGCRAQP PQDEETETPI GAVTHQGSHF