ID   TM225_BOVIN             Reviewed;         231 AA.
AC   Q32KQ5;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 2.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Transmembrane protein 225;
GN   Name=TMEM225; Synonyms=PMP22CD;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probably inhibits protein phosphatase 1 (PP1) in sperm via
CC       binding to catalytic subunit PPP1CC. {ECO:0000250|UniProtKB:Q9D9S2}.
CC   -!- SUBUNIT: Interacts (via RVxF motif) with PPP1CC.
CC       {ECO:0000250|UniProtKB:Q9D9S2}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC       membrane {ECO:0000250|UniProtKB:Q9D9S2}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- CAUTION: This protein is not related to the claudin family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI09976.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; BC109975; AAI09976.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001073753.2; NM_001080284.2.
DR   AlphaFoldDB; Q32KQ5; -.
DR   SMR; Q32KQ5; -.
DR   STRING; 9913.ENSBTAP00000044826; -.
DR   PaxDb; Q32KQ5; -.
DR   GeneID; 519985; -.
DR   KEGG; bta:519985; -.
DR   CTD; 338661; -.
DR   eggNOG; ENOG502TDTU; Eukaryota.
DR   InParanoid; Q32KQ5; -.
DR   OrthoDB; 1369226at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0002080; C:acrosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0010923; P:negative regulation of phosphatase activity; IEA:InterPro.
DR   InterPro; IPR033542; TMEM225.
DR   PANTHER; PTHR36477; PTHR36477; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..231
FT                   /note="Transmembrane protein 225"
FT                   /id="PRO_0000339349"
FT   TOPO_DOM        1..13
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        35..67
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        89..97
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        119..135
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        157..231
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   MOTIF           225..229
FT                   /note="RVxF"
FT                   /evidence="ECO:0000250|UniProtKB:Q9D9S2"
SQ   SEQUENCE   231 AA;  26224 MW;  28FB56CDDCD70AFB CRC64;
     MVHILVRKVE ATNMFFSSWT LVFLAVGIII EEWAELKLGP QKPTITHSPW ICCTPLWPSD
     GLEVIRNILI VVLSLSFMHN LLLGFEFTYM IPQTKYTLIM TACLAFLTGI LLLGALLLYH
     HMLRQGESVY YSSYKISWII FTAYLNVLFL FISGFLSLLQ YKQPIDGSGS LIPRSARKSQ
     VMEQHGVSIK VVSLPAGTAM PRSIVRLHSA HMKEDSPERL NIQARRVTWA L