ID   TM225_HUMAN             Reviewed;         225 AA.
AC   Q6GV28;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Transmembrane protein 225;
GN   Name=TMEM225; Synonyms=PMP22CD;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Huang C.Q., Wu S.L., Zhou J.L.;
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probably inhibits protein phosphatase 1 (PP1) in sperm via
CC       binding to catalytic subunit PPP1CC. {ECO:0000250|UniProtKB:Q9D9S2}.
CC   -!- SUBUNIT: Interacts (via RVxF motif) with PPP1CC.
CC       {ECO:0000250|UniProtKB:Q9D9S2}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC       membrane {ECO:0000250|UniProtKB:Q9D9S2}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- CAUTION: This protein is not related to the claudin family.
CC       {ECO:0000305}.
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DR   EMBL; AY634366; AAT47557.1; -; mRNA.
DR   CCDS; CCDS31697.1; -.
DR   RefSeq; NP_001013765.2; NM_001013743.2.
DR   AlphaFoldDB; Q6GV28; -.
DR   SMR; Q6GV28; -.
DR   BioGRID; 130776; 1.
DR   IntAct; Q6GV28; 1.
DR   STRING; 9606.ENSP00000364166; -.
DR   iPTMnet; Q6GV28; -.
DR   PhosphoSitePlus; Q6GV28; -.
DR   BioMuta; TMEM225; -.
DR   DMDM; 74709356; -.
DR   PaxDb; Q6GV28; -.
DR   PeptideAtlas; Q6GV28; -.
DR   PRIDE; Q6GV28; -.
DR   Antibodypedia; 18955; 2 antibodies from 2 providers.
DR   DNASU; 338661; -.
DR   Ensembl; ENST00000375026.7; ENSP00000364166.2; ENSG00000204300.7.
DR   GeneID; 338661; -.
DR   KEGG; hsa:338661; -.
DR   UCSC; uc001pzi.4; human.
DR   CTD; 338661; -.
DR   DisGeNET; 338661; -.
DR   GeneCards; TMEM225; -.
DR   HGNC; HGNC:32390; TMEM225.
DR   HPA; ENSG00000204300; Tissue enriched (testis).
DR   neXtProt; NX_Q6GV28; -.
DR   PharmGKB; PA164726548; -.
DR   VEuPathDB; HostDB:ENSG00000204300; -.
DR   eggNOG; ENOG502TDTU; Eukaryota.
DR   HOGENOM; CLU_074600_0_0_1; -.
DR   InParanoid; Q6GV28; -.
DR   OrthoDB; 1369226at2759; -.
DR   PhylomeDB; Q6GV28; -.
DR   TreeFam; TF339613; -.
DR   PathwayCommons; Q6GV28; -.
DR   SignaLink; Q6GV28; -.
DR   BioGRID-ORCS; 338661; 11 hits in 1063 CRISPR screens.
DR   GenomeRNAi; 338661; -.
DR   Pharos; Q6GV28; Tdark.
DR   PRO; PR:Q6GV28; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   RNAct; Q6GV28; protein.
DR   Bgee; ENSG00000204300; Expressed in sperm and 24 other tissues.
DR   ExpressionAtlas; Q6GV28; baseline and differential.
DR   Genevisible; Q6GV28; HS.
DR   GO; GO:0002080; C:acrosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0010923; P:negative regulation of phosphatase activity; IEA:InterPro.
DR   InterPro; IPR033542; TMEM225.
DR   PANTHER; PTHR36477; PTHR36477; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..225
FT                   /note="Transmembrane protein 225"
FT                   /id="PRO_0000339350"
FT   TOPO_DOM        1..8
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..72
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        94..99
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        121..136
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        158..225
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   MOTIF           219..223
FT                   /note="RVxF"
FT                   /evidence="ECO:0000250|UniProtKB:Q9D9S2"
FT   VARIANT         134
FT                   /note="N -> S (in dbSNP:rs10893099)"
FT                   /id="VAR_061690"
FT   VARIANT         196
FT                   /note="C -> R (in dbSNP:rs1939927)"
FT                   /id="VAR_051260"
SQ   SEQUENCE   225 AA;  25828 MW;  4A556833D82AC106 CRC64;
     MVHVSNRSIQ GMNILFSSWA VVLMVMGITL DKWVELISED ERAKMNHSPW MMCCPALWPE
     DDLKVVRIMM TSSLGLSFLL NLILGMKFTY LIPQNKYIQL FTTILSFFSG ISLLWALILY
     HNKLKQGQSM HFSNYRITWI MYTAYLNVFF LSVCGVLSLL ECKLSTSSCT CLNIHKSDNE
     CKESENSIED ISLPECTAMP RSIVRAHTVN SLNKKVQTRH VTWAL