TM225_MOUSE
ID TM225_MOUSE Reviewed; 230 AA.
AC Q9D9S2;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Transmembrane protein 225;
GN Name=Tmem225; Synonyms=Pmp22cd;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, INTERACTION WITH PPP1CC, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF 224-ARG--TRP-228.
RX PubMed=25605614; DOI=10.1002/mrd.22453;
RA Matsuura M., Yogo K.;
RT "TMEM225: a possible protein phosphatase 1gamma2 (PP1gamma2) regulator
RT localizes to the equatorial segment in mouse spermatozoa.";
RL Mol. Reprod. Dev. 82:139-148(2015).
CC -!- FUNCTION: Probably inhibits protein phosphatase 1 (PP1) in sperm via
CC binding to catalytic subunit PPP1CC. {ECO:0000269|PubMed:25605614}.
CC -!- SUBUNIT: Interacts (via RVxF motif) with PPP1CC.
CC {ECO:0000269|PubMed:25605614}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC membrane {ECO:0000269|PubMed:25605614}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in testis, epididymis and spermatozoa (at
CC protein level). Not expressed in brain, heart, lung, liver, spleen,
CC kidney and skeletal muscle. {ECO:0000269|PubMed:25605614}.
CC -!- DEVELOPMENTAL STAGE: Detected in testis 25 days after birth and
CC thereafter. {ECO:0000269|PubMed:25605614}.
CC -!- CAUTION: This protein is not related to the claudin family.
CC {ECO:0000305}.
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DR EMBL; AK006538; BAB24641.1; -; mRNA.
DR EMBL; BC116869; AAI16870.1; -; mRNA.
DR EMBL; BC116871; AAI16872.1; -; mRNA.
DR CCDS; CCDS52774.1; -.
DR RefSeq; NP_083655.1; NM_029379.1.
DR AlphaFoldDB; Q9D9S2; -.
DR SMR; Q9D9S2; -.
DR STRING; 10090.ENSMUSP00000036816; -.
DR PhosphoSitePlus; Q9D9S2; -.
DR PaxDb; Q9D9S2; -.
DR PRIDE; Q9D9S2; -.
DR ProteomicsDB; 259554; -.
DR Antibodypedia; 18955; 2 antibodies from 2 providers.
DR Ensembl; ENSMUST00000046333; ENSMUSP00000036816; ENSMUSG00000040541.
DR GeneID; 75667; -.
DR KEGG; mmu:75667; -.
DR UCSC; uc009oze.2; mouse.
DR CTD; 338661; -.
DR MGI; MGI:1922917; Tmem225.
DR VEuPathDB; HostDB:ENSMUSG00000040541; -.
DR eggNOG; ENOG502TDTU; Eukaryota.
DR GeneTree; ENSGT00390000011564; -.
DR HOGENOM; CLU_074600_0_0_1; -.
DR InParanoid; Q9D9S2; -.
DR OMA; KMNHSPW; -.
DR OrthoDB; 1369226at2759; -.
DR PhylomeDB; Q9D9S2; -.
DR TreeFam; TF339613; -.
DR BioGRID-ORCS; 75667; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q9D9S2; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q9D9S2; protein.
DR Bgee; ENSMUSG00000040541; Expressed in spermatid and 4 other tissues.
DR ExpressionAtlas; Q9D9S2; baseline and differential.
DR Genevisible; Q9D9S2; MM.
DR GO; GO:0002080; C:acrosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0010923; P:negative regulation of phosphatase activity; IEA:InterPro.
DR InterPro; IPR033542; TMEM225.
DR PANTHER; PTHR36477; PTHR36477; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..230
FT /note="Transmembrane protein 225"
FT /id="PRO_0000339351"
FT TOPO_DOM 1..8
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..71
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..139
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..230
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT MOTIF 224..228
FT /note="RVxF"
FT /evidence="ECO:0000269|PubMed:25605614"
FT MUTAGEN 224..228
FT /note="Missing: Abolishes interaction with PPP1CC."
FT /evidence="ECO:0000269|PubMed:25605614"
SQ SEQUENCE 230 AA; 26483 MW; 3A745D7E7BE1EAE6 CRC64;
MMHIPNRSIQ AANIFFSSGA ILLLIVGLIM EDWVELIPKV RKDKTTHSPW LGCCPPFWPE
ESLEVVRRIM RMTLNISIYL NLIIGLQFSY MISQNKCVHL LVGFLSFFAG CLLFYAIIVY
HHKLNKGQYV YFVNYKTKWI AFTVYLTIAL FLTCGIFCFI QSTNRCECMK FCIPHTESKS
QEMIPSTIEV VSLPPRCAMP RSIVHVHSVT SKDGSLNRPH TQARRVTWAL
