ID   TM225_RAT               Reviewed;         231 AA.
AC   Q6GV27;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Transmembrane protein 225;
GN   Name=Tmem225; Synonyms=Pmp22cd;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   STRAIN=Sprague-Dawley; TISSUE=Testis;
RX   PubMed=20979528; DOI=10.1089/dna.2010.1048;
RA   Yang S., Wang W., Lei C., Liu Q., Xu F., Xing X., Chen H., Liu J., Wu S.,
RA   Wang M.;
RT   "Localization and characterization of rat transmembrane protein 225
RT   specifically expressed in testis.";
RL   DNA Cell Biol. 30:9-16(2011).
CC   -!- FUNCTION: Probably inhibits protein phosphatase 1 (PP1) in sperm via
CC       binding to catalytic subunit PPP1CC. {ECO:0000250|UniProtKB:Q9D9S2}.
CC   -!- SUBUNIT: Interacts (via RVxF motif) with PPP1CC.
CC       {ECO:0000250|UniProtKB:Q9D9S2}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC       membrane {ECO:0000250|UniProtKB:Q9D9S2}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in testis, specifically in spermatocytes
CC       and round spermatids. {ECO:0000269|PubMed:20979528}.
CC   -!- DEVELOPMENTAL STAGE: Detected in adult testis from age 13 months
CC       onwards. {ECO:0000269|PubMed:20979528}.
CC   -!- CAUTION: This protein is not related to the claudin family.
CC       {ECO:0000305}.
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DR   EMBL; AY634367; AAT47558.1; -; mRNA.
DR   RefSeq; NP_001019530.1; NM_001024359.1.
DR   AlphaFoldDB; Q6GV27; -.
DR   STRING; 10116.ENSRNOP00000031308; -.
DR   iPTMnet; Q6GV27; -.
DR   PhosphoSitePlus; Q6GV27; -.
DR   PaxDb; Q6GV27; -.
DR   Ensembl; ENSRNOT00000084164; ENSRNOP00000074826; ENSRNOG00000054439.
DR   GeneID; 500980; -.
DR   KEGG; rno:500980; -.
DR   UCSC; RGD:1562358; rat.
DR   CTD; 338661; -.
DR   RGD; 1562358; Tmem225.
DR   eggNOG; ENOG502TDTU; Eukaryota.
DR   GeneTree; ENSGT00390000011564; -.
DR   HOGENOM; CLU_074600_0_0_1; -.
DR   InParanoid; Q6GV27; -.
DR   OMA; KMNHSPW; -.
DR   OrthoDB; 1369226at2759; -.
DR   PhylomeDB; Q6GV27; -.
DR   TreeFam; TF339613; -.
DR   PRO; PR:Q6GV27; -.
DR   Proteomes; UP000002494; Chromosome 8.
DR   Bgee; ENSRNOG00000054439; Expressed in testis.
DR   GO; GO:0002080; C:acrosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0010923; P:negative regulation of phosphatase activity; IEA:InterPro.
DR   InterPro; IPR033542; TMEM225.
DR   PANTHER; PTHR36477; PTHR36477; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..231
FT                   /note="Transmembrane protein 225"
FT                   /id="PRO_0000339352"
FT   TOPO_DOM        1..13
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        35..71
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        93..99
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        121..139
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        140..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        161..231
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   MOTIF           225..229
FT                   /note="RVxF"
FT                   /evidence="ECO:0000250|UniProtKB:Q9D9S2"
SQ   SEQUENCE   231 AA;  26598 MW;  EB8EA6DBB5F9CD9C CRC64;
     MMRIPNRSIQ AANIFFSSGA ILLLIAGLIM ENWVELIPKV RKDKVTHSPW LGCCPPFWPE
     ESLEAIRRMM MMSLNISIYL NLIIGLQFTY MISQNKCVHL LIGFLSFFTG CLLFYAIIVY
     HHKLNKGQYV YFVNYKTKWI VFTIYLTIAL FLTCGIFSFI QCTNRCACMK FCVPHTESSS
     KAMTQNTIQV ISLPPRSEMP RSIVHMHSDM PGKEGSISKP HLQSRRVTWA L