TM22_SOLLC
ID TM22_SOLLC Reviewed; 861 AA.
AC Q71BG9; C3UZH7; C3UZI2;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=ToMV resistance protein Tm-2(2) {ECO:0000303|PubMed:14558663, ECO:0000303|Ref.2};
DE AltName: Full=Disease resistance protein Tm-2(2) {ECO:0000303|Ref.3};
DE AltName: Full=ToMV resistance protein Tm-2a {ECO:0000303|PubMed:24173917};
GN Name=Tm-2(2) {ECO:0000303|PubMed:14558663, ECO:0000303|Ref.2};
GN Synonyms=Tm-2a {ECO:0000303|PubMed:24173917};
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=cv. ATV847, cv. Craigella GCR267, and cv. Moneymaker;
RX PubMed=14558663; DOI=10.1023/a:1025434519282;
RA Lanfermeijer F.C., Dijkhuis J., Sturre M.J.G., de Haan P., Hille J.;
RT "Cloning and characterization of the durable tomato mosaic virus resistance
RT gene Tm-2(2) from Lycopersicon esculentum.";
RL Plant Mol. Biol. 52:1037-1049(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. B;
RA Sui X., Shamimuzzaman M., Zheng Y., Simmons A.M., Fei Z., Wu Z.,
RA Ling K.-S.;
RT "Understanding the mechanism of resistance breaking on tomato by Tomato
RT mottle mosaic virus.";
RL Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 228-423 AND 594-827.
RC STRAIN=cv. Mogeor LA3471, cv. Royal Red Cherry LA2088, and
RC cv. VFNT Cherry LA1221;
RA Shi A., Vierling R., Grazzini R., Hogue P., Miller K.;
RT "Development of SNP markers for disease resistance genes in tomato.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION.
RX PubMed=24232612; DOI=10.1007/bf00305828;
RA Young N.D., Tanksley S.D.;
RT "RFLP analysis of the size of chromosomal segments retained around the Tm-2
RT locus of tomato during backcross breeding.";
RL Theor. Appl. Genet. 77:353-359(1989).
RN [5]
RP FUNCTION.
RX PubMed=1730937; DOI=10.1099/0022-1317-73-1-165;
RA Calder V.L., Palukaitis P.;
RT "Nucleotide sequence analysis of the movement genes of resistance breaking
RT strains of tomato mosaic virus.";
RL J. Gen. Virol. 73:165-168(1992).
RN [6]
RP FUNCTION.
RC STRAIN=cv. Craigella GCR267;
RX PubMed=8411345; DOI=10.1128/jvi.67.11.6432-6438.1993;
RA Weber H., Schultze S., Pfitzner A.J.P.;
RT "Two amino acid substitutions in the tomato mosaic virus 30-kilodalton
RT movement protein confer the ability to overcome the Tm-2(2) resistance gene
RT in the tomato.";
RL J. Virol. 67:6432-6438(1993).
RN [7]
RP GENE FAMILY.
RC STRAIN=cv. Craigella GCR267;
RX PubMed=24173917; DOI=10.1007/bf00221969;
RA Ohmori T., Murata M., Motoyoshi F.;
RT "Identification of RAPD markers linked to the Tm-2 locus in tomato.";
RL Theor. Appl. Genet. 90:307-311(1995).
RN [8]
RP FUNCTION.
RC STRAIN=cv. Craigella GCR267;
RX PubMed=9612948; DOI=10.1094/mpmi.1998.11.6.498;
RA Weber H., Pfitzner A.J.P.;
RT "Tm-2(2) resistance in tomato requires recognition of the carboxy terminus
RT of the movement protein of tomato mosaic virus.";
RL Mol. Plant Microbe Interact. 11:498-503(1998).
RN [9]
RP FUNCTION.
RC STRAIN=cv. Craigella GCR267;
RX PubMed=15290376; DOI=10.1007/s00705-004-0312-0;
RA Weber H., Ohnesorge S., Silber M.V., Pfitzner A.J.P.;
RT "The Tomato mosaic virus 30 kDa movement protein interacts differentially
RT with the resistance genes Tm-2 and Tm-2(2).";
RL Arch. Virol. 149:1499-1514(2004).
RN [10]
RP FUNCTION, AND GENE FAMILY.
RC STRAIN=cv. Craigella GCR267;
RX PubMed=16172136; DOI=10.1093/jxb/eri288;
RA Lanfermeijer F.C., Warmink J., Hille J.;
RT "The products of the broken Tm-2 and the durable Tm-2(2) resistance genes
RT from tomato differ in four amino acids.";
RL J. Exp. Bot. 56:2925-2933(2005).
RN [11]
RP FUNCTION.
RC STRAIN=cv. Craigella GCR267;
RX PubMed=17238011; DOI=10.1007/s00705-006-0915-8;
RA Strasser M., Pfitzner A.J.P.;
RT "The double-resistance-breaking Tomato mosaic virus strain ToMV1-2 contains
RT two independent single resistance-breaking domains.";
RL Arch. Virol. 152:903-914(2007).
RN [12]
RP FUNCTION, AND MUTAGENESIS OF ILE-257; MET-286; TYR-767 AND SER-769.
RX PubMed=21310506; DOI=10.1016/j.jplph.2011.01.002;
RA Kobayashi M., Yamamoto-Katou A., Katou S., Hirai K., Meshi T., Ohashi Y.,
RA Mitsuhara I.;
RT "Identification of an amino acid residue required for differential
RT recognition of a viral movement protein by the Tomato mosaic virus
RT resistance gene Tm-2(2).";
RL J. Plant Physiol. 168:1142-1145(2011).
RN [13]
RP FUNCTION.
RX PubMed=23148080; DOI=10.1104/pp.112.209213;
RA Zhao J., Liu Q., Zhang H., Jia Q., Hong Y., Liu Y.;
RT "The rubisco small subunit is involved in tobamovirus movement and Tm-2(2)-
RT mediated extreme resistance.";
RL Plant Physiol. 161:374-383(2013).
RN [14]
RP FUNCTION, INTERACTION WITH TOBACCO MOSAIC VIRUS MOUVEMENT PROTEIN
RP (MICROBIAL INFECTION), SUBCELLULAR LOCATION, AND MUTAGENESIS OF LYS-191 AND
RP ASP-481.
RX PubMed=28258211; DOI=10.1104/pp.16.01512;
RA Chen T., Liu D., Niu X., Wang J., Qian L., Han L., Liu N., Zhao J.,
RA Hong Y., Liu Y.;
RT "Antiviral resistance protein Tm-2(2) functions on the plasma membrane.";
RL Plant Physiol. 173:2399-2410(2017).
RN [15]
RP FUNCTION.
RC STRAIN=cv. T-5, and cv. Vendor;
RX PubMed=28107419; DOI=10.1371/journal.pone.0170429;
RA Luria N., Smith E., Reingold V., Bekelman I., Lapidot M., Levin I.,
RA Elad N., Tam Y., Sela N., Abu-Ras A., Ezra N., Haberman A., Yitzhak L.,
RA Lachman O., Dombrovsky A.;
RT "A new Israeli tobamovirus isolate infects tomato plants harboring Tm-2(2)
RT resistance genes.";
RL PLoS ONE 12:E0170429-E0170429(2017).
RN [16]
RP FUNCTION.
RX PubMed=29582165; DOI=10.1007/s00705-018-3819-5;
RA Maayan Y., Pandaranayaka E.P.J., Srivastava D.A., Lapidot M., Levin I.,
RA Dombrovsky A., Harel A.;
RT "Using genomic analysis to identify tomato Tm-2 resistance-breaking
RT mutations and their underlying evolutionary path in a new and emerging
RT tobamovirus.";
RL Arch. Virol. 163:1863-1875(2018).
RN [17]
RP INTERACTION WITH HSP90-1.
RX PubMed=29692788; DOI=10.3389/fpls.2018.00411;
RA Qian L., Zhao J., Du Y., Zhao X., Han M., Liu Y.;
RT "Hsp90 interacts with Tm-2(2) and is essential for Tm-22-mediated
RT resistance to tobacco mosaic virus.";
RL Front. Plant Sci. 9:411-411(2018).
CC -!- FUNCTION: Inhibitor of viral mouvements which confers resistance to
CC some tobamoviruses including tomato mosaic virus (ToMV) (e.g. strains
CC L, B7 and ToMV1-2) and tobacco mosaic virus (TMV), but not to
CC resistance-breaking isolates (e.g. LIIA and ToMV2(2)) ToMV and tomato
CC brown rugose fruit virus (ToBRFV) (PubMed:17238011, PubMed:14558663,
CC PubMed:1730937, PubMed:8411345, PubMed:9612948, PubMed:15290376,
CC PubMed:16172136, PubMed:21310506, PubMed:29582165, PubMed:28107419).
CC Elicits a hypersensitive reaction in response to avirulent (Avr)
CC movement proteins from resistance inducing tobamoviruses (e.g. ToMV and
CC TMV) strains, thus leading to programmed cell death; this local extreme
CC resistance requires rbcS (PubMed:15290376, PubMed:21310506,
CC PubMed:28258211, PubMed:23148080). {ECO:0000269|PubMed:14558663,
CC ECO:0000269|PubMed:15290376, ECO:0000269|PubMed:16172136,
CC ECO:0000269|PubMed:17238011, ECO:0000269|PubMed:1730937,
CC ECO:0000269|PubMed:21310506, ECO:0000269|PubMed:23148080,
CC ECO:0000269|PubMed:28107419, ECO:0000269|PubMed:28258211,
CC ECO:0000269|PubMed:29582165, ECO:0000269|PubMed:8411345,
CC ECO:0000269|PubMed:9612948}.
CC -!- SUBUNIT: (Microbial infection) Interacts with tobamoviruses mouvement
CC protein (e.g. tobacco mosaic virus (TMV) MP, AC P03583) at the plasma
CC membrane; this interaction triggers defense responses leading to
CC programmed cell death. {ECO:0000269|PubMed:28258211}.
CC -!- SUBUNIT: Binds to HSP90 proteins (e.g. HSP90-1 and Nicotiana
CC benthamiana HSP90-1); this interaction seems required for defense
CC responses toward tobamoviruses. {ECO:0000269|PubMed:29692788}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28258211};
CC Peripheral membrane protein {ECO:0000269|PubMed:28258211}; Cytoplasmic
CC side {ECO:0000305|PubMed:28258211}.
CC -!- MISCELLANEOUS: The Tm-2, Tm-2(2) and Tm-2nv alleles present in the
CC tomato mosaic virus (ToMV/TMV)-resistant tomato cv. Craigella isolates
CC GCR236 (AC Q71BH0), cv. Craigella GCR267 (AC Q71BG9) and cv. Yukang 2
CC (AC Q5MLE9), respectively confers resistance to ToMV but not the tm-2
CC allele present in the ToMV-susceptible tomato cv. Craigella isolate
CC GCR26 (AC Q71BH1). {ECO:0000269|PubMed:14558663}.
CC -!- SIMILARITY: Belongs to the disease resistance NB-LRR family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF536201; AAQ10736.1; -; Genomic_DNA.
DR EMBL; KY910827; AVQ09319.1; -; Genomic_DNA.
DR EMBL; FJ817597; ACO52370.1; -; Genomic_DNA.
DR EMBL; FJ817602; ACO52375.1; -; Genomic_DNA.
DR EMBL; FJ817603; ACO52376.1; -; Genomic_DNA.
DR EMBL; FJ817606; ACO52379.1; -; Genomic_DNA.
DR AlphaFoldDB; Q71BG9; -.
DR SMR; Q71BG9; -.
DR Proteomes; UP000004994; Unplaced.
DR ExpressionAtlas; Q71BG9; baseline and differential.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IDA:UniProtKB.
DR GO; GO:0009626; P:plant-type hypersensitive response; IDA:UniProtKB.
DR CDD; cd14798; RX-CC_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038005; RX-like_CC.
DR InterPro; IPR041118; Rx_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR23155; PTHR23155; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF18052; Rx_N; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Coiled coil; Host-virus interaction;
KW Hypersensitive response; Leucine-rich repeat; Membrane; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..861
FT /note="ToMV resistance protein Tm-2(2)"
FT /id="PRO_0000448716"
FT DOMAIN 162..388
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 225..248
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 305..327
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 388..411
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 449..472
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 510..536
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 585..608
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 609..631
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 652..680
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 689..710
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 712..735
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 736..758
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REPEAT 784..810
FT /note="LRR 12"
FT /evidence="ECO:0000255"
FT REPEAT 811..835
FT /note="LRR 13"
FT /evidence="ECO:0000255"
FT COILED 63..83
FT /evidence="ECO:0000255"
FT BINDING 185..192
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT SITE 767
FT /note="Contributes to the specific recognition of ToMV-B7
FT viral mouvement protein and subsequent resistance to ToMV-
FT B7"
FT /evidence="ECO:0000269|PubMed:21310506"
FT MUTAGEN 191
FT /note="K->R: Partially soluble, disturbed subcellular
FT localization."
FT /evidence="ECO:0000269|PubMed:28258211"
FT MUTAGEN 257
FT /note="I->F: ToMV-B7 viral mouvement protein-dependent
FT induced cell death."
FT /evidence="ECO:0000269|PubMed:21310506"
FT MUTAGEN 286
FT /note="M->I: ToMV-B7 viral mouvement protein-dependent
FT induced cell death."
FT /evidence="ECO:0000269|PubMed:21310506"
FT MUTAGEN 481
FT /note="D->V: Autoactive, constitutive cell death induction
FT activity and normal plasma membrane localization."
FT /evidence="ECO:0000269|PubMed:28258211"
FT MUTAGEN 767
FT /note="Y->N: Impaired ToMV-B7 viral mouvement protein-
FT dependent induced cell death."
FT /evidence="ECO:0000269|PubMed:21310506"
FT MUTAGEN 769
FT /note="S->T: ToMV-B7 viral mouvement protein-dependent
FT induced cell death."
FT /evidence="ECO:0000269|PubMed:21310506"
SQ SEQUENCE 861 AA; 98786 MW; 58CE0E121B17F504 CRC64;
MAEILLTSVI NKSVEIAGNL LIQEGKRLYW LKEDIDWLQR EMRHIRSYVD NAKAKEAGGD
SRVKNLLKDI QELAGDVEDL LDDFLPKIQQ SNKFNYCLKR SSFADEFAME IEKIKRRVVD
IDRIRKTYNI IDTDNNNDDC VLLDRRRLFL HADETEIIGL DDDFNMLQAK LLNQDLHYGV
VSIVGMPGLG KTTLAKKLYR LIRDQFECSG LVYVSQQPRA SEILLDIAKQ IGLTEQKMKE
NLEDNLRSLL KIKRYVILLD DIWDVEIWDD LKLVLPECDS KVGSRMIITS RNSNVGRYIG
GESSLHALQP LESEKSFELF TKKIFNFDDN NSWANASPDL VNIGRNIVGR CGGIPLAIVV
TAGMLRARER TEHAWNRVLE SMGHKVQDGC AKVLALSYND LPIASRPCFL YFGLYPEDHE
IRAFDLINMW IAEKFIVVNS GNRREAEDLA EDVLNDLVSR NLIQLAKRTY NGRISSCRIH
DLLHSLCVDL AKESNFFHTA HDAFGDPGNV ARLRRITFYS DNVMIEFFRS NPKLEKLRVL
FCFAKDPSIF SHMAYFDFKL LHTLVVVMSQ SFQAYVTIPS KFGNMTCLRY LRLEGNICGK
LPNSIVKLTR LETIDIDRRS LIQPPSGVWE SKHLRHLCYR DYGQACNSCF SISSFYPNIY
SLHPNNLQTL MWIPDKFFEP RLLHRLINLR KLGILGVSNS TVKMLSIFSP VLKALEVLKL
SFSSDPSEQI KLSSYPHIAK LHLNVNRTMA LNSQSFPPNL IKLTLAYFSV DRYILAVLKT
FPKLRKLKMF ICKYNEEKMD LSGEANGYSF PQLEVLHIHS PNGLSEVTCT DDVSMPKLKK
LLLTGFHCRI SLSERLKKLS K
