ID   BSTA_STRSQ              Reviewed;          46 AA.
AC   K4JY29;
DT   28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Bottromycin D {ECO:0000303|PubMed:22984777};
DE   Flags: Precursor;
GN   Name=bstA {ECO:0000303|PubMed:22984777};
OS   Streptomyces sp.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1931;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DETERMINATION OF BOTTROMYCIN D
RP   STRUCTURE, MASS SPECTROMETRY, AND MUTAGENESIS OF ALA-4.
RC   STRAIN=WMMB 272;
RX   PubMed=22984777; DOI=10.1021/ol3022758;
RA   Hou Y., Tianero M.D., Kwan J.C., Wyche T.P., Michel C.R., Ellis G.A.,
RA   Vazquez-Rivera E., Braun D.R., Rose W.E., Schmidt E.W., Bugni T.S.;
RT   "Structure and biosynthesis of the antibiotic bottromycin D.";
RL   Org. Lett. 14:5050-5053(2012).
CC   -!- FUNCTION: Bottromycin D is a ribosomally synthesized and post-
CC       translationally modified peptide (RiPP) that displays antibiotic
CC       activity against methicillin-resistant S.aureus (MRSA).
CC       {ECO:0000269|PubMed:22984777}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- PTM: The precursor peptide is first ribosomally synthesized and then
CC       highly tailored by specific enzymes to yield the final natural product.
CC       These modifications include several methylations, cyclization and the
CC       formation of t-Leu and Thia-beta-Ala residues.
CC       {ECO:0000269|PubMed:22984777}.
CC   -!- MASS SPECTROMETRY: Mass=795.42; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:22984777};
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DR   EMBL; JX827389; AFU90404.1; -; Genomic_DNA.
DR   AlphaFoldDB; K4JY29; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Secreted.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000305|PubMed:22984777"
FT   CHAIN           2..9
FT                   /note="Bottromycin D"
FT                   /id="PRO_0000443309"
FT   PROPEP          10..46
FT                   /evidence="ECO:0000305|PubMed:22984777"
FT                   /id="PRO_0000443310"
FT   MUTAGEN         4
FT                   /note="A->V: Leads to the production of bottromycin A2
FT                   instead of bottromycin D."
FT                   /evidence="ECO:0000269|PubMed:22984777"
SQ   SEQUENCE   46 AA;  5083 MW;  2ED98BE0D08BD9DF CRC64;
     MGPAVVFDCM TADFLNDDPN NAELSSLEME ELESWGAWSD DTDQSV