TM254_HUMAN
ID TM254_HUMAN Reviewed; 123 AA.
AC Q8TBM7; D3DWC8; Q53HP4; Q5JTC0; Q5JTC1; Q6IA45; Q9H8S6;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Transmembrane protein 254;
GN Name=TMEM254; Synonyms=C10orf57;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Adipose tissue;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- INTERACTION:
CC Q8TBM7; Q13520: AQP6; NbExp=3; IntAct=EBI-11956809, EBI-13059134;
CC Q8TBM7; Q3SXY8: ARL13B; NbExp=3; IntAct=EBI-11956809, EBI-11343438;
CC Q8TBM7; O15342: ATP6V0E1; NbExp=3; IntAct=EBI-11956809, EBI-12935759;
CC Q8TBM7; Q9BXK5: BCL2L13; NbExp=3; IntAct=EBI-11956809, EBI-747430;
CC Q8TBM7; Q13323: BIK; NbExp=3; IntAct=EBI-11956809, EBI-700794;
CC Q8TBM7; P04233-2: CD74; NbExp=3; IntAct=EBI-11956809, EBI-12222807;
CC Q8TBM7; P11912: CD79A; NbExp=3; IntAct=EBI-11956809, EBI-7797864;
CC Q8TBM7; Q9HA82: CERS4; NbExp=3; IntAct=EBI-11956809, EBI-2622997;
CC Q8TBM7; Q7Z7G2: CPLX4; NbExp=3; IntAct=EBI-11956809, EBI-18013275;
CC Q8TBM7; Q15125: EBP; NbExp=3; IntAct=EBI-11956809, EBI-3915253;
CC Q8TBM7; Q9GZR5: ELOVL4; NbExp=3; IntAct=EBI-11956809, EBI-18535450;
CC Q8TBM7; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-11956809, EBI-18304435;
CC Q8TBM7; Q14318: FKBP8; NbExp=3; IntAct=EBI-11956809, EBI-724839;
CC Q8TBM7; Q8TBE3: FNDC9; NbExp=3; IntAct=EBI-11956809, EBI-12142257;
CC Q8TBM7; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-11956809, EBI-13345167;
CC Q8TBM7; Q8TED1: GPX8; NbExp=3; IntAct=EBI-11956809, EBI-11721746;
CC Q8TBM7; P38484: IFNGR2; NbExp=3; IntAct=EBI-11956809, EBI-3905457;
CC Q8TBM7; P26951: IL3RA; NbExp=3; IntAct=EBI-11956809, EBI-1757512;
CC Q8TBM7; A8MZ59: LEUTX; NbExp=3; IntAct=EBI-11956809, EBI-17490413;
CC Q8TBM7; Q8TAF8: LHFPL5; NbExp=3; IntAct=EBI-11956809, EBI-2820517;
CC Q8TBM7; Q9GZY8-5: MFF; NbExp=3; IntAct=EBI-11956809, EBI-11956541;
CC Q8TBM7; Q8N4V1: MMGT1; NbExp=3; IntAct=EBI-11956809, EBI-6163737;
CC Q8TBM7; Q9H2K0: MTIF3; NbExp=3; IntAct=EBI-11956809, EBI-3923617;
CC Q8TBM7; O75459: PAGE1; NbExp=3; IntAct=EBI-11956809, EBI-2559100;
CC Q8TBM7; P60201-2: PLP1; NbExp=3; IntAct=EBI-11956809, EBI-12188331;
CC Q8TBM7; Q9H6H4: REEP4; NbExp=3; IntAct=EBI-11956809, EBI-7545592;
CC Q8TBM7; Q86VR2: RETREG3; NbExp=3; IntAct=EBI-11956809, EBI-10192441;
CC Q8TBM7; Q9NR31: SAR1A; NbExp=3; IntAct=EBI-11956809, EBI-3920694;
CC Q8TBM7; Q9BY50: SEC11C; NbExp=3; IntAct=EBI-11956809, EBI-2855401;
CC Q8TBM7; P27105: STOM; NbExp=3; IntAct=EBI-11956809, EBI-1211440;
CC Q8TBM7; Q16623: STX1A; NbExp=3; IntAct=EBI-11956809, EBI-712466;
CC Q8TBM7; P21579: SYT1; NbExp=3; IntAct=EBI-11956809, EBI-524909;
CC Q8TBM7; Q6PL24: TMED8; NbExp=3; IntAct=EBI-11956809, EBI-11603430;
CC Q8TBM7; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-11956809, EBI-8638294;
CC Q8TBM7; Q96Q45-2: TMEM237; NbExp=3; IntAct=EBI-11956809, EBI-10982110;
CC Q8TBM7; Q96B21: TMEM45B; NbExp=3; IntAct=EBI-11956809, EBI-3923061;
CC Q8TBM7; Q9NW97: TMEM51; NbExp=3; IntAct=EBI-11956809, EBI-726044;
CC Q8TBM7; Q9BSE2: TMEM79; NbExp=3; IntAct=EBI-11956809, EBI-8649725;
CC Q8TBM7; Q9Y320: TMX2; NbExp=3; IntAct=EBI-11956809, EBI-6447886;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8TBM7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TBM7-2; Sequence=VSP_047062;
CC Name=3;
CC IsoId=Q8TBM7-3; Sequence=VSP_047063;
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DR EMBL; AK023325; BAB14528.1; -; mRNA.
DR EMBL; CR457310; CAG33591.1; -; mRNA.
DR EMBL; AK222536; BAD96256.1; -; mRNA.
DR EMBL; AL356095; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471142; EAW80412.1; -; Genomic_DNA.
DR EMBL; CH471142; EAW80413.1; -; Genomic_DNA.
DR EMBL; BC022252; AAH22252.1; -; mRNA.
DR CCDS; CCDS58086.1; -. [Q8TBM7-3]
DR CCDS; CCDS58087.1; -. [Q8TBM7-2]
DR CCDS; CCDS7363.1; -. [Q8TBM7-1]
DR RefSeq; NP_001257296.1; NM_001270367.1.
DR RefSeq; NP_001257297.1; NM_001270368.1.
DR RefSeq; NP_001257298.1; NM_001270369.1.
DR RefSeq; NP_001257299.1; NM_001270370.1. [Q8TBM7-3]
DR RefSeq; NP_001257300.1; NM_001270371.1.
DR RefSeq; NP_001257301.1; NM_001270372.1. [Q8TBM7-2]
DR RefSeq; NP_001257302.1; NM_001270373.1. [Q8TBM7-2]
DR RefSeq; NP_001257303.1; NM_001270374.1.
DR RefSeq; NP_079401.2; NM_025125.3. [Q8TBM7-1]
DR AlphaFoldDB; Q8TBM7; -.
DR BioGRID; 123168; 43.
DR IntAct; Q8TBM7; 40.
DR STRING; 9606.ENSP00000361355; -.
DR iPTMnet; Q8TBM7; -.
DR PhosphoSitePlus; Q8TBM7; -.
DR BioMuta; TMEM254; -.
DR EPD; Q8TBM7; -.
DR jPOST; Q8TBM7; -.
DR MassIVE; Q8TBM7; -.
DR MaxQB; Q8TBM7; -.
DR PaxDb; Q8TBM7; -.
DR PeptideAtlas; Q8TBM7; -.
DR PRIDE; Q8TBM7; -.
DR Antibodypedia; 29924; 80 antibodies from 18 providers.
DR DNASU; 80195; -.
DR Ensembl; ENST00000372274.5; ENSP00000361348.1; ENSG00000133678.14. [Q8TBM7-3]
DR Ensembl; ENST00000372275.5; ENSP00000361349.1; ENSG00000133678.14. [Q8TBM7-2]
DR Ensembl; ENST00000372277.7; ENSP00000361351.3; ENSG00000133678.14. [Q8TBM7-3]
DR Ensembl; ENST00000372281.8; ENSP00000361355.3; ENSG00000133678.14. [Q8TBM7-1]
DR GeneID; 80195; -.
DR KEGG; hsa:80195; -.
DR MANE-Select; ENST00000372281.8; ENSP00000361355.3; NM_025125.4; NP_079401.2.
DR UCSC; uc001kbl.5; human. [Q8TBM7-1]
DR CTD; 80195; -.
DR DisGeNET; 80195; -.
DR GeneCards; TMEM254; -.
DR HGNC; HGNC:25804; TMEM254.
DR HPA; ENSG00000133678; Low tissue specificity.
DR neXtProt; NX_Q8TBM7; -.
DR OpenTargets; ENSG00000133678; -.
DR PharmGKB; PA134875855; -.
DR VEuPathDB; HostDB:ENSG00000133678; -.
DR eggNOG; ENOG502S4F5; Eukaryota.
DR GeneTree; ENSGT00390000016042; -.
DR HOGENOM; CLU_150378_1_0_1; -.
DR InParanoid; Q8TBM7; -.
DR OMA; LLYKGWW; -.
DR OrthoDB; 1399874at2759; -.
DR PhylomeDB; Q8TBM7; -.
DR TreeFam; TF328617; -.
DR PathwayCommons; Q8TBM7; -.
DR SignaLink; Q8TBM7; -.
DR BioGRID-ORCS; 80195; 7 hits in 1070 CRISPR screens.
DR ChiTaRS; TMEM254; human.
DR GenomeRNAi; 80195; -.
DR Pharos; Q8TBM7; Tdark.
DR PRO; PR:Q8TBM7; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q8TBM7; protein.
DR Bgee; ENSG00000133678; Expressed in right uterine tube and 157 other tissues.
DR ExpressionAtlas; Q8TBM7; baseline and differential.
DR Genevisible; Q8TBM7; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR028110; TMEM254.
DR PANTHER; PTHR34104; PTHR34104; 1.
DR Pfam; PF14934; DUF4499; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..123
FT /note="Transmembrane protein 254"
FT /id="PRO_0000089796"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT VAR_SEQ 85..123
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_047062"
FT VAR_SEQ 85..123
FT /note="HKGITSGRAQLLWFLQTFFFGIASLTILIAYKRKRQKQT -> QNELRIRIK
FT MRKLILRDSE (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_047063"
FT CONFLICT 56
FT /note="H -> R (in Ref. 3; BAD96256)"
FT /evidence="ECO:0000305"
FT CONFLICT 76
FT /note="S -> F (in Ref. 2; CAG33591)"
FT /evidence="ECO:0000305"
FT CONFLICT 81
FT /note="V -> A (in Ref. 1; BAB14528)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 123 AA; 14243 MW; 56BE16322D749904 CRC64;
MATAAGATYF QRGSLFWFTV ITLSFGYYTW VVFWPQSIPY QNLGPLGPFT QYLVDHHHTL
LCNGYWLAWL IHVGESLYAI VLCKHKGITS GRAQLLWFLQ TFFFGIASLT ILIAYKRKRQ
KQT
